Construct: ORF TRCN0000468178
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001419.1_s317c1
- Derived from:
- ccsbBroadEn_08009
- DNA Barcode:
- GGCGTTCTCATGGAACCTGAAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXO22 (26263)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468178
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26263 | FBXO22 | F-box protein 22 | NM_147188.3 | 99.9% | 99.7% | 1205C>T |
| 2 | human | 26263 | FBXO22 | F-box protein 22 | XM_011521447.3 | 74.1% | 73.9% | 0_1ins312;893C>T |
| 3 | human | 26263 | FBXO22 | F-box protein 22 | NM_012170.3 | 68% | 65.7% | (many diffs) |
| 4 | human | 26263 | FBXO22 | F-box protein 22 | NR_037623.2 | 9.9% | (many diffs) | |
| 5 | mouse | 71999 | Fbxo22 | F-box protein 22 | NM_028049.2 | 88.1% | 92% | (many diffs) |
| 6 | mouse | 71999 | Fbxo22 | F-box protein 22 | XM_006511459.2 | 66.5% | 69.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1275
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gccggtaggc tgctgcggcg agtgccgcgg ctcctccgta gacccgcgga 121 gcaccttcgt gttgagtaac ctggcggagg tggtggagcg tgtgctcacc ttcctgcccg 181 ccaaggcgtt gctgcgggtg gcctgcgtgt gccgcttatg gagggagtgt gtgcgcagag 241 tattgcggac ccatcggagc gtaacctgga tctccgcagg cctggcggag gccggccacc 301 tggaggggca ttgcttggtt cgcgtggtag cagaggagct tgagaatgtt cgcatcttac 361 cacatacagt tctttacatg gctgattcag aaactttcat tagtctggaa gagtgtcgtg 421 gccataagag agcaaggaaa agaactagta tggaaacagc acttgccctt gagaagctat 481 tccccaaaca atgccaagtc cttgggattg tgaccccagg aattgtagtg actccaatgg 541 gatcaggtag caatcgacct caggaaatag aaattggaga atctggtttt gctttattat 601 tccctcaaat tgaaggaata aaaatacaac cctttcattt tattaaggat ccaaagaatt 661 taacattaga aagacatcaa ctcactgaag taggtctttt agataaccct gaacttcgtg 721 tggtccttgt ctttggttat aattgctgta aggtgggagc cagtaattat ctgcagcaag 781 tagtcagcac tttcagtgat atgaatatca tcttggctgg aggccaggtg gacaacctgt 841 catcactgac ttctgaaaag aaccctctgg atattgatgc cTCGGGTGTG GTTGGACTGT 901 CATTTAGTGG ACACCGAATC CAGAGTGCCA CTGTGCTCCT CAACGAGGAC GTCAGTGATG 961 AGAAGACTGC TGAGGCTGCG ATGCAGCGCC TCAAAGCGGC CAACATTCCA GAGCATAACA 1021 CCATTGGCTT CATGTTTGCA TGCGTTGGCA GGGGCTTTCA GTATTACAGA GCCAAGGGGA 1081 ATGTTGAGGC TGATGCATTT AGAAAGTTTT TTCCTAGTGT TCCCTTATTC GGCTTCTTTG 1141 GAAATGGAGA AATTGGATGT GATCGGATAG TCACTGGGAA CTTTATATTG AGGAAATGTA 1201 ATGAGGTAAA AGATGATGAT CTGTTTCATA GCTATACAAC AATAATGGCA CTCATACATC 1261 TGGGGTCATT TAAATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGCGTTCTCA TGGAACCTGA 1441 AACCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt