Construct: ORF TRCN0000468192
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003300.1_s317c1
- Derived from:
- ccsbBroadEn_15788
- DNA Barcode:
- CACGTACTCACAGTCCTTGGCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH3YL1 (26751)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468192
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26751 | SH3YL1 | SH3 and SYLF domain contain... | NM_001159597.2 | 34.1% | 30% | (many diffs) |
| 2 | human | 26751 | SH3YL1 | SH3 and SYLF domain contain... | NM_015677.4 | 32.2% | 28.3% | (many diffs) |
| 3 | human | 26751 | SH3YL1 | SH3 and SYLF domain contain... | NR_104225.1 | 16.2% | (many diffs) | |
| 4 | human | 26751 | SH3YL1 | SH3 and SYLF domain contain... | NR_104226.1 | 13.6% | 1_774delinsA;784C>A;1116_2497del | |
| 5 | human | 26751 | SH3YL1 | SH3 and SYLF domain contain... | NR_104224.2 | 10.7% | 1_198del;209C>A;541_3165del | |
| 6 | human | 26751 | SH3YL1 | SH3 and SYLF domain contain... | NR_104227.1 | 10.2% | 1_1046delinsA;1056C>A;1388_3319del | |
| 7 | human | 26751 | SH3YL1 | SH3 and SYLF domain contain... | NR_104223.1 | 9.7% | (many diffs) | |
| 8 | mouse | 24057 | Sh3yl1 | Sh3 domain YSC-like 1 | NM_013709.5 | 29% | 27.9% | (many diffs) |
| 9 | mouse | 24057 | Sh3yl1 | Sh3 domain YSC-like 1 | XR_872408.1 | 20% | (many diffs) | |
| 10 | mouse | 24057 | Sh3yl1 | Sh3 domain YSC-like 1 | XR_872407.1 | 19.3% | (many diffs) | |
| 11 | mouse | 24057 | Sh3yl1 | Sh3 domain YSC-like 1 | NR_110343.1 | 15.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 408
- ORF length:
- 342
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa taaccatata ccttccaatt tGAAATCAGA AGCAAAAAAG GCTGCCAAAA 121 TATTAAGAGA ATTCACAGAA ATAACTTCCA GAAATGGACC TGATAAGATC ATTCCTGCTC 181 ACGTAATTGC GAAGGCTAAA GGCCTTGCAA TTCTGTCTGT GATCAAAGCC GGGTTCCTGG 241 TGACTGCCAG AGGAGGCAGC GGGATTGTAG TGGCGCGCCT TCCAGATGGA AAATGGTCTG 301 CACCCTCAGC CATTGGGATA GCTGGCCTTG GTGGAGGATT TGAAATAGGA ATTGAGACAC 361 ATTTACAGCT ACATATTCCC TCTGAGCATT GCCCTGGCTG CCTTCCATAC CCAACTTTCT 421 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 481 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 541 GTGGAAAGGA CGACACGTAC TCACAGTCCT TGGCTACACG CGTTAAGTCg acaatcaacc 601 tctggattac aaaatttgtg aaagatt