Construct: ORF TRCN0000468260
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010039.1_s317c1
- Derived from:
- ccsbBroadEn_07718
- DNA Barcode:
- TCCGCGATCCCTGGATCACATGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FERMT2 (10979)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468260
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10979 | FERMT2 | fermitin family member 2 | NM_006832.3 | 99.9% | 100% | 885C>T |
| 2 | human | 10979 | FERMT2 | fermitin family member 2 | NM_001134999.1 | 98.9% | 98.9% | 885C>T;1602_1622del |
| 3 | human | 10979 | FERMT2 | fermitin family member 2 | XM_005267285.3 | 98.3% | 98.4% | 525_557del;918C>T |
| 4 | human | 10979 | FERMT2 | fermitin family member 2 | XM_006720010.3 | 97.7% | 97.8% | 885C>T;1602_1646del |
| 5 | human | 10979 | FERMT2 | fermitin family member 2 | XM_006720009.3 | 97.3% | 97.4% | 525_557del;918C>T;1635_1655del |
| 6 | human | 10979 | FERMT2 | fermitin family member 2 | XM_006720008.3 | 96.2% | 96.3% | 525_557del;918C>T;1635_1679del |
| 7 | human | 10979 | FERMT2 | fermitin family member 2 | NM_001135000.2 | 90.8% | 90.8% | (many diffs) |
| 8 | mouse | 218952 | Fermt2 | fermitin family member 2 | NM_146054.2 | 89.4% | 98.2% | (many diffs) |
| 9 | mouse | 218952 | Fermt2 | fermitin family member 2 | XM_006518828.2 | 88.5% | 97.2% | (many diffs) |
| 10 | mouse | 218952 | Fermt2 | fermitin family member 2 | XM_006518827.2 | 88% | 96.6% | (many diffs) |
| 11 | mouse | 218952 | Fermt2 | fermitin family member 2 | XM_006518826.2 | 87.1% | 95.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2106
- ORF length:
- 2040
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctggacggg ataaggatgc cagatggctg ctacgcggac gggacgtggg 121 aactgagtgt ccatgtgacg gacctgaacc gcgatgtcac cctgagagtg accggcgagg 181 tgcacattgg aggcgtgatg cttaagctgg tggagaaact cgatgtaaaa aaagattggt 241 ctgaccatgc tctctggtgg gaaaagaaga gaacttggct tctgaagaca cattggacct 301 tagataagta tggtattcag gcagatgcta agcttcagtt cacccctcag cacaaactgc 361 tccgcctgca gcttcccaac atgaagtatg tgaaggtgaa agtgaatttc tctgatagag 421 tcttcaaagc tgtttctgac atctgtaaga cttttaatat cagacacccc gaagaacttt 481 ctctcttaaa gaaacccaga gatccaacaa agaaaaaaaa gaagaagcta gatgaccagt 541 ctgaagatga ggcacttgaa ttagaggggc ctcttatcac tcctggatca ggaagtatat 601 attcaagccc aggactgtat agtaaaacaa tgacccccac ttatgatgct catgatggaa 661 gccccttgtc accaacttct gcttggtttg gtgacagtgc tttgtcagaa ggcaatcctg 721 gtatacttgc tgtcagtcaa ccaatcacgt caccagaaat cttggcaaaa atgttcaagc 781 ctcaagctct tcttgataaa gcaaaaatca accaaggatg gcttgattcc tcaagatctc 841 tcatggaaca agatgtgaag gaaaatgagg ccttgctgct ccgattcaag tattacagct 901 tttttgattt gaatccaaag tatgatgcaa tcagaatcaa tcagctttat gagcaggcca 961 aatgggccat tctcctggaa gagattgaat gcacagaaga agaaatgatg atgtttgcag 1021 ccctgcagta tcatatcaat aagctgtcaa tcatgacatc agagaatcat ttgaacaaca 1081 gtgacaaaga agttgatgaa gttgatgctg ccctttcaga cctggagatt actctggaag 1141 ggggtaaaac gtcaacaatt ttgggtgaca ttacttccat tcctgaactt gctgactaca 1201 ttaaagtttt caagccaaaa aagctgactc tgaaaggtta caaacaatat tggtgcacct 1261 tcaaagacac atccatttct tgttataaga gcaaagaaga atccagtggc acaccagctc 1321 atcagatgaa cctcagggga tgtgaagtta ccccagatgt aaacatttca ggccaaaaat 1381 ttaacattaa actcctgatt ccagttgcag aaggcatgaa tgaaatctgg cttcgttgtg 1441 acaatgaaaa acagtatgca cactggatgg cagcctgcag attagcctcc aaaggcaaga 1501 ccatggcgga cagttcttac aacttagaag ttcagaatat tctttccttt ctgaagatgc 1561 agcatttaaa cccagatcct cagttaatac cagagcagat cacgactgat ataactcctg 1621 aatgtttggt gtctccccgc tatctaaaaa agtataagaa caagcagata acagcgagaa 1681 tcttggaggc ccatcagaat gtagctcaga tgagtctaat tgaagccaag atgagattta 1741 ttcaagcttg gcagtcacta cctgaatttg gcatcactca cttcattgca aggttccaag 1801 ggggcaaaaa agaagaactt attggaattg catacaacag actgattcGG ATGGATGCCA 1861 GCACTGGAGA TGCAATTAAA ACATGGCGTT TCAGCAACAT GAAACAGTGG AATGTCAACT 1921 GGGAAATCAA AATGGTCACC GTAGAGTTTG CAGATGAAGT ACGATTGTCC TTCATTTGTA 1981 CTGAAGTAGA TTGCAAAGTG GTTCATGAAT TCATTGGTGG CTACATATTT CTCTCAACAC 2041 GTGCAAAAGA CCAAAACGAG AGTTTAGATG AAGAGATGTT CTACAAACTT ACCAGTGGTT 2101 GGGTGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2161 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2221 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCCGCGATC CCTGGATCAC ATGCAACGCG 2281 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt