Transcript: Human XM_005267285.3

PREDICTED: Homo sapiens fermitin family member 2 (FERMT2), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FERMT2 (10979)
Length:
3321
CDS:
140..2215

Additional Resources:

NCBI RefSeq record:
XM_005267285.3
NBCI Gene record:
FERMT2 (10979)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005267285.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000127808 GCCTTACCAGACCAGTTAATA pLKO.1 2337 3UTR 100% 15.000 21.000 N FERMT2 n/a
2 TRCN0000330231 GCCTTACCAGACCAGTTAATA pLKO_005 2337 3UTR 100% 15.000 21.000 N FERMT2 n/a
3 TRCN0000330301 GTAGCTCAGATGAGTCTAATT pLKO_005 1808 CDS 100% 13.200 18.480 N FERMT2 n/a
4 TRCN0000330300 AGCAGATCACGACTGATATAA pLKO_005 1701 CDS 100% 15.000 10.500 N FERMT2 n/a
5 TRCN0000128511 CCGAAGAACTTTCTCTCTTAA pLKO.1 543 CDS 100% 13.200 9.240 N FERMT2 n/a
6 TRCN0000127493 CCAGGGCTTAACCTATATGAA pLKO.1 2544 3UTR 100% 5.625 3.938 N FERMT2 n/a
7 TRCN0000128058 GCGGACAGTTCTTACAACTTA pLKO.1 1613 CDS 100% 5.625 3.938 N FERMT2 n/a
8 TRCN0000330230 GCGGACAGTTCTTACAACTTA pLKO_005 1613 CDS 100% 5.625 3.938 N FERMT2 n/a
9 TRCN0000129672 CGACTGATATAACTCCTGAAT pLKO.1 1710 CDS 100% 4.950 3.465 N FERMT2 n/a
10 TRCN0000330302 CAGCACTGGAGATGCAATTAA pLKO_005 1966 CDS 100% 15.000 9.000 N FERMT2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005267285.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07718 pDONR223 100% 98.3% 98.4% None 525_557del;918C>T n/a
2 ccsbBroad304_07718 pLX_304 0% 98.3% 98.4% V5 525_557del;918C>T n/a
3 TRCN0000468260 TCCGCGATCCCTGGATCACATGCA pLX_317 13% 98.3% 98.4% V5 525_557del;918C>T n/a
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