Construct: ORF TRCN0000468296
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005537.1_s317c1
- Derived from:
- ccsbBroadEn_14174
- DNA Barcode:
- GAAAAGTAAGGACTTAGACTTGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CDKAL1 (54901)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468296
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_017010986.1 | 21.7% | 2% | 14_17delCCTG;285_286insGGT;289_1305del |
| 2 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_011514719.2 | 21.3% | 2% | 14_17delCCTG;285_286insGGT;289_1329del |
| 3 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_011514718.1 | 16.9% | 1.6% | 14_17delCCTG;285_286insGGT;289_1674del |
| 4 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | NM_017774.3 | 16.3% | 1.5% | 14_17delCCTG;285_286insGGT;289_1737del |
| 5 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_024446481.1 | 15.9% | .8% | 14_17delCCTG;285_286insGGT;289_1779del |
| 6 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743501.1 | 9.3% | (many diffs) | |
| 7 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743500.1 | 9.1% | (many diffs) | |
| 8 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_926267.2 | 7.6% | (many diffs) | |
| 9 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_926265.2 | 7.4% | (many diffs) | |
| 10 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743495.2 | 7.4% | (many diffs) | |
| 11 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_926266.2 | 7.2% | (many diffs) | |
| 12 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743496.2 | 6.7% | (many diffs) | |
| 13 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | NM_001308486.1 | 60.3% | 1.9% | (many diffs) |
| 14 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | XM_011244342.2 | 19% | 2% | (many diffs) |
| 15 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | NM_144536.3 | 14.2% | .6% | (many diffs) |
| 16 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | NR_131896.1 | 7.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 174
- ORF length:
- 84
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCTTCTGC ATTGATACAC TACTGGATGA 121 CATCGAAGAT ATCGTGTCTC AGGAAGATTC AAAACCACAA GATAGGCATT TTGTAAGAAA 181 GGATGTTGTC CCGAAGGTAC GAAGGCGAAA TACCCAAAAA TATTTGCAAG AGGAAGAAAA 241 CAGTCCACCA AGTGACAGCA CTATTCCAGG CATACAGAAA ATTTGGATAC GAACATGGGG 301 TTGTTCTCAT AATAATTCAG ATGGAGAATA TATGGCTGGA CAGCTAGCTG CTTATGGCTA 361 TAAAATTACA GGTGAATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 421 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 481 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGAAAAGTA AGGACTTAGA 541 CTTGGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt