Construct: ORF TRCN0000468356
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013108.1_s317c1
- Derived from:
- ccsbBroadEn_13957
- DNA Barcode:
- ACACGCCCTTTGTTTTCATCCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC11A1 (6556)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468356
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6556 | SLC11A1 | solute carrier family 11 me... | XM_005246794.4 | 99.8% | 99.7% | 471A>G;1169T>C |
| 2 | human | 6556 | SLC11A1 | solute carrier family 11 me... | XM_006712709.4 | 99.8% | 99.7% | 471A>G;1169T>C |
| 3 | human | 6556 | SLC11A1 | solute carrier family 11 me... | XM_006712710.4 | 99.8% | 99.7% | 471A>G;1169T>C |
| 4 | human | 6556 | SLC11A1 | solute carrier family 11 me... | XM_011511684.3 | 96.6% | 82.5% | (many diffs) |
| 5 | human | 6556 | SLC11A1 | solute carrier family 11 me... | XM_011511685.3 | 96.6% | 82.5% | (many diffs) |
| 6 | human | 6556 | SLC11A1 | solute carrier family 11 me... | XM_006712711.4 | 92.6% | 92.5% | 0_1ins93;378A>G;1076T>C |
| 7 | human | 6556 | SLC11A1 | solute carrier family 11 me... | XM_005246793.4 | 87.8% | 87.1% | (many diffs) |
| 8 | human | 6556 | SLC11A1 | solute carrier family 11 me... | XM_017004766.2 | 87.8% | 87.1% | (many diffs) |
| 9 | human | 6556 | SLC11A1 | solute carrier family 11 me... | XM_017004765.2 | 83.3% | 82.7% | (many diffs) |
| 10 | human | 6556 | SLC11A1 | solute carrier family 11 me... | NM_000578.4 | 77.2% | 76.5% | (many diffs) |
| 11 | human | 6556 | SLC11A1 | solute carrier family 11 me... | XM_017004767.2 | 55% | 54.1% | (many diffs) |
| 12 | human | 6556 | SLC11A1 | solute carrier family 11 me... | XR_427107.3 | 28.2% | (many diffs) | |
| 13 | human | 6556 | SLC11A1 | solute carrier family 11 me... | XR_427108.4 | 26.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1362
- ORF length:
- 1296
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcgatgtcag ctcactgcaa cctctacctc ccaggtgccc cgcaccgtcc 121 tctggctgac catcgagcta gccattgtgg gctccgacat gcaggaagtc atcggcacgg 181 ccattgcatt caatctgctc tcagctggac gaatcccact ctggggtggc gtcctcatca 241 ccatcgtgga caccttcttc ttcctcttcc tcgataacta cgggctgcgg aagctggaag 301 ctttttttgg actccttata accattatgg ccttgacctt tggctatgag tatgtggtgg 361 cgcgtcctga gcagggagcg cttcttcggg gcctgttcct gccctcgtgc ccgggctgcg 421 gccaccccga gctgctgcag gcggtgggca ttgttggcgc catcatcatg ccccacaaca 481 tctacctgca ctcggccctg gtcaagtctc gagagataga ccgggcccgc cgagcggaca 541 tcagagaagc caacatgtac ttcctgattg aggccaccat cgccctgtcc gtctccttta 601 tcatcaacct ctttgtcatg gctgtctttg ggcaggcctt ctaccagaaa accaaccagg 661 ctgcgttcaa catctgtgcc aacagcagcc tccacgacta cgccaagatc ttccccatga 721 acaacgccac cgtggccgtg gacatttacc aggggggcgt gatcctgggc tgcctgttcg 781 gccccgcggc cctctacatc tgggccatag gtctcctggc ggctgggcag agctccacca 841 tgacgggcac ctacgcggga cagttcgtga tggagggctt cctgaggctg cggtggtcac 901 gcttcgcccg tgtcctcctc acccgctcct gcgccatcct gcccaccgtg ctcgtggctg 961 tcttccggga cctgagggac ttgtcgggcc tcaatgatct gctcaacgtg ctgcagagcc 1021 tgctgctccc gttcgccgtg ctgcccatcc tcacgttcac cagcatgccc acccTCATGC 1081 AGGAGTTTGC CAATGGCCTG CTGAACAAGG TCGTCACCTC TTCCATCATG GTGCTAGTCT 1141 GCGCCATCAA CCTCTACTTC GTGGTCAGCT ATCTGCCCAG CCTGCCCCAC CCTGCCTACT 1201 TCGGCCTTGC AGCCTTGCTG GCCGCAGCCT ACCCGGGCCT CAGCACCTAC CTGGTCTGGA 1261 CCTGTTGCCT TGCCCACGGA GCCACCTTTC TGGCCCACAG CTCCCACCAC CACTTCCTGT 1321 ATGGGCTCCT TGAAGAGGAC CAGAAAGGGG AGACCTCTGG CTACCCAACT TTCTTGTACA 1381 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1441 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1501 AGGACGAACA CGCCCTTTGT TTTCATCCGA CACGCGTTAA GTCgacaatc aacctctgga 1561 ttacaaaatt tgtgaaagat t