Construct: ORF TRCN0000468373
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000076.1_s317c1
- Derived from:
- ccsbBroadEn_07035
- DNA Barcode:
- TTTGGCTTTTTCACCGCCTACTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBX15 (6913)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468373
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6913 | TBX15 | T-box transcription factor 15 | NM_152380.3 | 99.9% | 100% | 1392C>T |
| 2 | human | 6913 | TBX15 | T-box transcription factor 15 | NM_001330677.2 | 82.3% | 82.3% | 1_318del;1710C>T |
| 3 | human | 6913 | TBX15 | T-box transcription factor 15 | XM_005271161.4 | 78% | 77.9% | 1_318del;1025_1123del;1809C>T |
| 4 | human | 6913 | TBX15 | T-box transcription factor 15 | XM_011542052.2 | 54.1% | 54% | 0_1ins627;80_178del;864C>T |
| 5 | mouse | 21384 | Tbx15 | T-box 15 | XM_006501279.3 | 92.9% | 98.9% | (many diffs) |
| 6 | mouse | 21384 | Tbx15 | T-box 15 | XM_006501280.3 | 92.9% | 98.9% | (many diffs) |
| 7 | mouse | 21384 | Tbx15 | T-box 15 | NM_009323.2 | 76.5% | 81.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1557
- ORF length:
- 1488
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcttccatg gaggagattc aggtggagct gcaatgtgct gacctctgga 121 agcggttcca tgatattgga actgaaatga tcatcaccaa agcaggcagg aggatgtttc 181 ctgccatgag agtgaaaatc actggcctag atccacatca gcagtactac atagcaatgg 241 acattgtgcc tgtggacaat aaaagataca gatatgtgta tcatagctcc aagtggatgg 301 tggctggcaa tgctgattcc cctgtgcccc caagagttta tatacaccct gattctctag 361 cttctggaga cacctggatg agacaggtgg tcagttttga caaactcaag cttaccaaca 421 atgagttgga tgatcaagga catatcattc tgcactctat gcacaaatac cagcctcgag 481 ttcatgtgat tcgcaaagac ttcagcagtg acctttcacc cactaagcct gttcctgttg 541 gggatggggt gaaaacgttc aactttcctg agactgtgtt caccacagtt acggcctatc 601 agaatcagca gattaccaga ttaaaaattg accgaaaccc ttttgctaaa ggattcagag 661 attctgggag aaacagaact ggacttgaag ccatcatgga gacatatgca ttctggagac 721 ctcctgtgcg cacactcacc ttcgaagact tcaccaccat gcagaagcag caaggaggca 781 gcacaggcac ttccccaacc acctccagca ctgggacacc atccccttcg gcttcttctc 841 atcttttatc tccatcctgt tctcctccaa cttttcatct ggcccccaac actttcaatg 901 tgggctgccg agaaagccag ctgtgtaatc taaacctctc tgattatcca ccatgtgccc 961 gaagcaacat ggctgccttg cagagctacc cagggctgag tgacagtggc tacaacaggc 1021 ttcagagtgg caccacttca gccactcagc cctctgaaac cttcatgcct cagaggactc 1081 catccctgat ctcaggaata ccaactcctc cctcgttgcc tggcaacagc aagatggaag 1141 cctacggtgg ccagctgggg TCCTTTCCCA CTTCCCAGTT TCAGTATGTC ATGCAGGCAG 1201 GCAATGCTGC CTCCAGCTCC TCATCACCAC ACATGTTCGG GGGCAGCCAC ATGCAGCAGA 1261 GCTCCTACAA TGCCTTCTCC CTTCACAACC CTTACAACCT GTATGGATAC AATTTCCCCA 1321 CTTCCCCTAG GCTAGCTGCA AGCCCGGAAA AACTGAGCGC CTCTCAAAGC ACTTTACTCT 1381 GTTCTTCTCC TTCCAACGGG GCCTTTGGAG AGAGGCAGTA CCTGCCGTCA GGGATGGAGC 1441 ACAGCATGCA CATGATTAGT CCTTCACCCA ATAACCAACA GGCAACCAAC ACTTGTGATG 1501 GCCGGCAGTA TGGGGCAGTT CCAGGCTCCT CCTCCCAGAT GTCCGTGCAC ATGGTTTTGC 1561 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1621 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1681 ATATATCTTG TGGAAAGGAC GATTTGGCTT TTTCACCGCC TACTGAACGC GTTAAGTCga 1741 caatcaacct ctggattaca aaatttgtga aagatt