Construct: ORF TRCN0000468386
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011404.1_s317c1
- Derived from:
- ccsbBroadEn_01398
- DNA Barcode:
- ATTCCCATCTACATCCAGAAAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHEB (6009)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468386
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6009 | RHEB | Ras homolog, mTORC1 binding | NM_005614.4 | 100% | 100% | |
| 2 | human | 6009 | RHEB | Ras homolog, mTORC1 binding | XM_011516457.2 | 91.5% | 91.3% | (many diffs) |
| 3 | human | 6009 | RHEB | Ras homolog, mTORC1 binding | XM_024446854.1 | 91.5% | 91.3% | (many diffs) |
| 4 | human | 439965 | SHLD2P3 | shieldin complex subunit 2 ... | NR_160660.1 | 23.1% | (many diffs) | |
| 5 | human | 439965 | SHLD2P3 | shieldin complex subunit 2 ... | NR_160661.1 | 21.3% | (many diffs) | |
| 6 | mouse | 19744 | Rheb | Ras homolog enriched in brain | NM_053075.3 | 92.5% | 98.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gcagtccaag tcccggaaga tcgcgatcct gggctaccgg tctgtgggga 121 aatcctcatt gacgattcaa tttgttgaag gccaatttgt ggactcctac gatccaacca 181 tagaaaacac ttttacaaag ttgatcacag taaatggaca agaatatcat cttcaacttg 241 tagacacagc cgggcaagat gaatattcta tctttccTCA GACATACTCC ATAGATATTA 301 ATGGCTATAT TCTTGTGTAT TCTGTTACAT CAATCAAAAG TTTTGAAGTG ATTAAAGTTA 361 TCCATGGCAA ATTGTTGGAT ATGGTGGGGA AAGTACAAAT ACCTATTATG TTGGTTGGGA 421 ATAAGAAAGA CCTGCATATG GAAAGGGTGA TCAGTTATGA AGAAGGGAAA GCTTTGGCAG 481 AATCTTGGAA TGCAGCTTTT TTGGAATCTT CTGCTAAAGA AAATCAGACT GCTGTGGATG 541 TTTTTCGAAG GATAATTTTG GAGGCAGAAA AAATGGACGG GGCAGCTTCA CAAGGCAAGT 601 CTTCATGCTC GGTGATGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 661 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 721 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATTCCCA TCTACATCCA 781 GAAAAACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt