Construct: ORF TRCN0000468390
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010313.1_s317c1
- Derived from:
- ccsbBroadEn_08685
- DNA Barcode:
- ACCATGACGAGTCAAGCCACGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKR1B10 (57016)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468390
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57016 | AKR1B10 | aldo-keto reductase family ... | NM_020299.5 | 99.8% | 99.6% | 937A>G |
| 2 | human | 441282 | AKR1B15 | aldo-keto reductase family ... | NM_001367821.1 | 95.2% | 91.7% | (many diffs) |
| 3 | human | 57016 | AKR1B10 | aldo-keto reductase family ... | XM_011516416.1 | 87.5% | 87.3% | 230_231ins117;820A>G |
| 4 | human | 441282 | AKR1B15 | aldo-keto reductase family ... | NM_001080538.3 | 85.2% | 78.7% | (many diffs) |
| 5 | human | 441282 | AKR1B15 | aldo-keto reductase family ... | NM_001367820.1 | 85.2% | 78.7% | (many diffs) |
| 6 | human | 441282 | AKR1B15 | aldo-keto reductase family ... | XM_017012224.2 | 85.2% | 78.7% | (many diffs) |
| 7 | human | 57016 | AKR1B10 | aldo-keto reductase family ... | XR_927491.1 | 57.8% | (many diffs) | |
| 8 | human | 57016 | AKR1B10 | aldo-keto reductase family ... | XM_011516417.2 | 49% | 47.9% | (many diffs) |
| 9 | human | 441282 | AKR1B15 | aldo-keto reductase family ... | NM_001367822.1 | 30.9% | 26.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1014
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cacgtttgtg gagctcagta ccaaagccaa gatgcccatt gtgggcctgg 121 gcacttggaa gtctcctctt ggcaaagtga aagaagcagt gaaggtggcc attgatgcag 181 gatatcggca cattgactgt gcctatgtct atcagaatga acatgaagtg ggggaagcca 241 tccaagagaa gatccaagag aaggctgtga agcgggagga cctgttcatc gtcagcaagt 301 tgtggcccac tttctttgag agaccccttg tgaggaaagc ctttgagaag accctcaagg 361 acctgaagct gagctatctg gacgtctatc ttattcactg gccacaggga ttcaagtctg 421 gggatgacct tttccccaaa gatgataaag gtaatgccat cggtggaaaa gcaacgttct 481 tggatgcctg ggaggccatg gaggagctgg tggatgaggg gctggtgaaa gcccttgggg 541 tctccaattt cagccacttc cagatcgaga agctcttgaa caaacctgga ctgaaatata 601 aaccagtgac taaccaggtt gagtgtcacc catacctcac acaggagaaa ctgatccagt 661 actgcCACTC CAAGGGCATC ACCGTTACGG CCTACAGCCC CCTGGGCTCT CCGGATAGAC 721 CTTGGGCCAA GCCAGAAGAC CCTTCCCTGC TGGAGGATCC CAAGATTAAG GAGATTGCTG 781 CAAAGCACAA AAAAACCGCA GCCCAGGTTC TGATCCGTTT CCATATCCAG AGGAATGTGA 841 TTGTCATCCC CAAGTCTGTG ACACCAGCAC GCATTGTTGA GAACATTCAG GTCTTTGACT 901 TTAAATTGAG TGATGAGGAG ATGGCAACCA TACTCAGCTT CAACAGAAAC TGGAGGGCCT 961 GTAACGTGTT GCAATCCTCT CATTTGGAAG ACTATCCCTT CGATGCAGAA TATTGCCCAA 1021 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1081 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1141 TATCTTGTGG AAAGGACGAA CCATGACGAG TCAAGCCACG CCCACGCGTT AAGTCgacaa 1201 tcaacctctg gattacaaaa tttgtgaaag att