Construct: ORF TRCN0000468452
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005861.1_s317c1
- Derived from:
- ccsbBroadEn_02425
- DNA Barcode:
- TTGCTATTACCCGTTAAAAGCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDIPT (10423)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468452
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10423 | CDIPT | CDP-diacylglycerol--inosito... | NM_006319.5 | 100% | 100% | |
| 2 | human | 10423 | CDIPT | CDP-diacylglycerol--inosito... | NM_001286585.2 | 78.8% | 78.8% | 42_43ins135 |
| 3 | human | 10423 | CDIPT | CDP-diacylglycerol--inosito... | XM_005255038.4 | 78.8% | 70.8% | (many diffs) |
| 4 | human | 10423 | CDIPT | CDP-diacylglycerol--inosito... | NM_001286586.2 | 69.4% | 69.4% | 0_1ins195 |
| 5 | mouse | 52858 | Cdipt | CDP-diacylglycerol--inosito... | NM_026638.3 | 89.5% | 96.2% | (many diffs) |
| 6 | mouse | 52858 | Cdipt | CDP-diacylglycerol--inosito... | XM_006508022.2 | 86.6% | 93.1% | (many diffs) |
| 7 | mouse | 52858 | Cdipt | CDP-diacylglycerol--inosito... | NM_001347656.1 | 67.8% | 64.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc agacgaaaat atcttcctgt tcgtgcccaa cctcatcggt tatgcccgga 121 ttgtcttcgc catcatttct ttctacttca tgccctgctg ccccctcacg gcctcctcct 181 tctacctgct cagcggcctg ctggacgctt tcgatggaca cgctgctcgc gctcttaatc 241 aaggaacccg gtttggggcc atgctggaca tgctgacgga ccgctgctcc accatgtgcc 301 tgttggtcaa cctggccctg ctgtaccctG GAGCCACGCT GTTCTTCCAA ATCAGCATGA 361 GTTTGGATGT GGCCAGTCAC TGGCTGCACC TCCACAGTTC TGTGGTCCGA GGCAGTGAGA 421 GTCACAAGAT GATCGACTTG TCCGGGAATC CGGTGCTTCG GATCTACTAC ACCTCGAGGC 481 CTGCTCTGTT CACCTTGTGT GCTGGGAATG AGCTCTTCTA CTGCCTCCTC TACCTGTTCC 541 ATTTCTCTGA GGGACCTTTA GTTGGCTCTG TGGGACTGTT CCGGATGGGC CTCTGGGTCA 601 CTGCCCCCAT CGCCTTGCTG AAGTCGCTCA TCAGCGTCAT CCACCTGATC ACGGCCGCCC 661 GCAACATGGC TGCCCTGGAC GCAGCAGACC GCGCCAAGAA GAAGTGCCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA TTGCTATTAC CCGTTAAAAG CTTCACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt