Construct: ORF TRCN0000468463
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016040.1_s317c1
- Derived from:
- ccsbBroadEn_15538
- DNA Barcode:
- GCACGTAACCATTAAAGTGCCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PKN2 (5586)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468463
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5586 | PKN2 | protein kinase N2 | XM_011541772.2 | 9.1% | 9.1% | 1_1794del |
| 2 | human | 5586 | PKN2 | protein kinase N2 | NM_001320708.2 | 7.2% | 7.2% | 1_2301del |
| 3 | human | 5586 | PKN2 | protein kinase N2 | XM_017001783.2 | 6.5% | 6.5% | 1_2580del |
| 4 | human | 5586 | PKN2 | protein kinase N2 | XM_017001782.2 | 6.5% | 6.5% | 1_2586del |
| 5 | human | 5586 | PKN2 | protein kinase N2 | NM_001320707.1 | 6.4% | 6.4% | 1_2628del |
| 6 | human | 5586 | PKN2 | protein kinase N2 | NM_001320709.2 | 6.1% | 6.1% | 1_2724del |
| 7 | human | 5586 | PKN2 | protein kinase N2 | NM_006256.4 | 6% | 6% | 1_2772del |
| 8 | mouse | 109333 | Pkn2 | protein kinase N2 | NM_178654.4 | 5.7% | 5.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 213
- ORF end:
- 393
- ORF length:
- 180
- Sequence:
-
1 TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA 61 CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA 121 GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GCTAGCATCG 181 ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCATGGACAA AAAAGTAAAG CCACCATTTA 241 TACCTACCAT AAGAGGACGA GAAGATGTTA GTAATTTTGA TGATGAATTT ACCTCAGAAG 301 CACCTATTCT GACTCCACCT CGAGAACCAA GGATACTTTC GGAAGAGGAG CAGGAAATGT 361 TCAGAGATTT TGACTACATT GCTGATTGGT GTTACCCAAC TTTCTTGTAC AAAGTGGTTG 421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC 541 ACGTAACCAT TAAAGTGCCT CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 601 ttgtgaaaga tt