Construct: ORF TRCN0000468477
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012106.1_s317c1
- Derived from:
- ccsbBroadEn_11851
- DNA Barcode:
- CTGAAGCTCCACCCAAAAACGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGO2 (27161)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468477
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27161 | AGO2 | argonaute RISC catalytic co... | XM_017013317.2 | 74.6% | 74.8% | (many diffs) |
| 2 | human | 27161 | AGO2 | argonaute RISC catalytic co... | XM_011516966.3 | 71.8% | 71.9% | (many diffs) |
| 3 | human | 27161 | AGO2 | argonaute RISC catalytic co... | NM_012154.5 | 67.9% | 68.1% | (many diffs) |
| 4 | human | 27161 | AGO2 | argonaute RISC catalytic co... | XM_011516965.2 | 66.8% | 67% | (many diffs) |
| 5 | human | 27161 | AGO2 | argonaute RISC catalytic co... | NM_001164623.3 | 64% | 64.1% | (many diffs) |
| 6 | human | 27161 | AGO2 | argonaute RISC catalytic co... | XM_011516968.2 | 63.6% | 63.7% | (many diffs) |
| 7 | mouse | 239528 | Ago2 | argonaute RISC catalytic su... | NM_153178.4 | 61.4% | 68% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1821
- ORF length:
- 1755
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaggaagtac cgtgtctgca atgtgacccg gcggcccgcc agtcaccaaa 121 cattcccgct gcagcaggag agcgggcaga cggtggagtg cacggtggcc cagtatttca 181 aggacaggca caagttggtt ctgcgctacc cccacctccc atgtttacaa gtcggacagg 241 agcagaaaca cacctacctt cccctggagg tctgtaacat tgtggcagga caaagatgta 301 ttaaaaaatt aacggacaat cagacctcaa ccatgatcag agcaactgct aggtcggcgc 361 ccgatcggca agaagagatt agcaaattga tgcgaagtgc aagtttcaac acagatccat 421 acgtccgtga atttggaatc atggtcaaag atgagatgac agacgtgact gggcgggtgc 481 tgcagccgcc ctccatcctc tacgggggca ggaataaagc tattgcgacc cctgtccagg 541 gcgtctggga catgcggaac aagcagttcc acacgggcat cgagatcaag gtgtgggcca 601 ttgcgtgctt cgccccccag cgccagtgca cggaagtcca tctgaagtcc ttcacagagc 661 agctcagaaa gatctcgaga gacgctggca tgcccatcca gggccagccg tgcttctgca 721 aatacgcgca gggggcggac agcgtggagc ccatgttccg gcacctgaag aacacgtatg 781 cgggcctgca gctggtggtg gtcatcctgc ccggcaagac gcccgtgtac gccgaggtca 841 agcgcgtggg agacacggtg ctggggatgg ccacgcagtg cgtgcagatg aagaacgtgc 901 agaggaccac gccacagacc ctgtccaacc tctgcctgaa gatcaacgtc aagctgggag 961 gcgtgaacaa catcctgctg ccccagggca ggccgccggt gttccagcag cccgtcatct 1021 ttctgggagc agacgtcact cacccccccg ccggggatgg gaagaagccc tccattgccg 1081 ccgtggtggg cagcatggac gcccacccca atcgctactg cgccaccgtg cgcgtgcagc 1141 agcaccggca ggagatcata caagacctgg ccgccatggt ccgcgagctc ctcatccagt 1201 tctacaagtc cacgcgcttc aagcccaccc gcatcatctt ctaccgcgac ggtgtctctg 1261 aaggccagtt ccagcaggtt ctccaccacg agttgctggc catccgtgag gcctgtatca 1321 agctagaaaa agactaccag cccgggatca ccttcatcgt ggtgcagaag aggcaccaca 1381 cccggctctt ctgcactgac aagaacgagc gggttgggaa aagtggaaac attccagcag 1441 gcacgactgt ggacacgaaa atcacccacc ccaccgagtt cgacttctac ctgtgtagtc 1501 acgctggcat ccaggggaca agcaggcctt cgcactatca cgtcctctgg gacgacaatc 1561 gtttctcctc TGATGAGCTG CAGATCCTAA CCTACCAGCT GTGTCACACC TACGTGCGCT 1621 GCACACGCTC CGTGTCCATC CCAGCGCCAG CATACTACGC TCACCTGGTG GCCTTCCGGG 1681 CCAGGTACCA CCTGGTGGAT AAGGAACATG ACAGTGCTGA AGGAAGCCAT ACCTCTGGGC 1741 AGAGTAACGG GCGAGACCAC CAAGCACTGG CCAAGGCGGT CCAGGTTCAC CAAGACACTC 1801 TGCGCACCAT GTACTTTGCT TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1861 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1921 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTGA AGCTCCACCC 1981 AAAAACGCGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt