Construct: ORF TRCN0000468569

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002514.1_s317c1
Derived from:
ccsbBroadEn_10803
DNA Barcode:
AAAGGCACATGGAGATCTATATTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EPHA7 (2045)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468569
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2045 EPHA7 EPH receptor A7 NM_001288630.1 99.7% 99.2% 412A>G;832C>T
2 human 2045 EPHA7 EPH receptor A7 XM_017010366.2 63% 62.6% (many diffs)
3 human 2045 EPHA7 EPH receptor A7 XM_005248671.3 45.6% 45.4% (many diffs)
4 human 2045 EPHA7 EPH receptor A7 XM_017010365.2 28.1% 28% (many diffs)
5 human 2045 EPHA7 EPH receptor A7 NM_001288629.2 28% 27.8% (many diffs)
6 human 2045 EPHA7 EPH receptor A7 XM_005248669.3 28% 27.8% (many diffs)
7 human 2045 EPHA7 EPH receptor A7 NM_004440.4 27.9% 27.7% (many diffs)
8 human 2045 EPHA7 EPH receptor A7 XR_001743218.2 10.5% (many diffs)
9 mouse 13841 Epha7 Eph receptor A7 XM_017319954.1 42.8% 44.6% (many diffs)
10 mouse 13841 Epha7 Eph receptor A7 NM_001122889.1 42.4% 44.2% (many diffs)
11 mouse 13841 Epha7 Eph receptor A7 XM_006537607.3 41.3% 43.1% (many diffs)
12 mouse 13841 Epha7 Eph receptor A7 XM_017319955.1 31% 32.4% (many diffs)
13 mouse 13841 Epha7 Eph receptor A7 XM_006537606.3 26% 27.1% (many diffs)
14 mouse 13841 Epha7 Eph receptor A7 NM_001290434.1 26% 27.1% (many diffs)
15 mouse 13841 Epha7 Eph receptor A7 NM_010141.4 25.9% 27% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
903
ORF length:
837
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt ttttcaaact cggtaccctt catggattat tttatgctac atctggctgc
121tccgctttgc acacacaggg gaggcgcagg ctgcgaagga agtactactg ctggattcta
181aagcacaaca aacagagttg gagtggattt cctctccacc caatgggtgg gaagaaatta
241gtggtttgga tgagaactat accccgatac gaacatacca ggtgtgccaa gtcatggagc
301ccaaccaaaa caactggctg cggactaact ggatttccaa aggcaatgca caaaggattt
361ttgtagaatt gaaattcacc ctgagggatt gtaacagtct tcctggagta ctgggaactt
421gcaaggaaac atttaatttg tactattatg aaacagacta tgacactggC AGGAATGTAA
481GAGAAAACCT CTATGTAAAA ATAGACACCA TTGCTGCAGA TGAAAGTTTT ACCCAAGGTG
541ACCTTGGTGA AAGAAAGATG AAGCTTAACA CTGAGGTGAG AGAGATTGGA CCTTTGTCCA
601AAAAGGGATT CTATCTTGCC TTTCAGGATG TAGGGGCTTG CATAGCTTTG GTTTCTGTCA
661AAGTGTACTA CAAGAAGTGC TGGTCCATTA TTGAGAACTT AGCTATCTTT CCAGATACAG
721TGACTGGTTC AGAATTTTCC TCTTTAGTCG AGGTTCGAGG GACATGTGTC AGCAGTGCAG
781AGGAAGAAGC GGAAAACGCC CCCAGGATGC ACTGCAGTGC AGAAGGAGAA TGGTTAGTGC
841CCATTGGAAA ATGTATCTGC AAAGCAGGCT ACCAGCAAAA AGGAGACACT TGTGAATGTA
901AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
961TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1021GGCTTTATAT ATCTTGTGGA AAGGACGAAA AGGCACATGG AGATCTATAT TTACGCGTTA
1081AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)