Construct: ORF TRCN0000468569
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002514.1_s317c1
- Derived from:
- ccsbBroadEn_10803
- DNA Barcode:
- AAAGGCACATGGAGATCTATATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHA7 (2045)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468569
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2045 | EPHA7 | EPH receptor A7 | NM_001288630.1 | 99.7% | 99.2% | 412A>G;832C>T |
| 2 | human | 2045 | EPHA7 | EPH receptor A7 | XM_017010366.2 | 63% | 62.6% | (many diffs) |
| 3 | human | 2045 | EPHA7 | EPH receptor A7 | XM_005248671.3 | 45.6% | 45.4% | (many diffs) |
| 4 | human | 2045 | EPHA7 | EPH receptor A7 | XM_017010365.2 | 28.1% | 28% | (many diffs) |
| 5 | human | 2045 | EPHA7 | EPH receptor A7 | NM_001288629.2 | 28% | 27.8% | (many diffs) |
| 6 | human | 2045 | EPHA7 | EPH receptor A7 | XM_005248669.3 | 28% | 27.8% | (many diffs) |
| 7 | human | 2045 | EPHA7 | EPH receptor A7 | NM_004440.4 | 27.9% | 27.7% | (many diffs) |
| 8 | human | 2045 | EPHA7 | EPH receptor A7 | XR_001743218.2 | 10.5% | (many diffs) | |
| 9 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_017319954.1 | 42.8% | 44.6% | (many diffs) |
| 10 | mouse | 13841 | Epha7 | Eph receptor A7 | NM_001122889.1 | 42.4% | 44.2% | (many diffs) |
| 11 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_006537607.3 | 41.3% | 43.1% | (many diffs) |
| 12 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_017319955.1 | 31% | 32.4% | (many diffs) |
| 13 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_006537606.3 | 26% | 27.1% | (many diffs) |
| 14 | mouse | 13841 | Epha7 | Eph receptor A7 | NM_001290434.1 | 26% | 27.1% | (many diffs) |
| 15 | mouse | 13841 | Epha7 | Eph receptor A7 | NM_010141.4 | 25.9% | 27% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 903
- ORF length:
- 837
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ttttcaaact cggtaccctt catggattat tttatgctac atctggctgc 121 tccgctttgc acacacaggg gaggcgcagg ctgcgaagga agtactactg ctggattcta 181 aagcacaaca aacagagttg gagtggattt cctctccacc caatgggtgg gaagaaatta 241 gtggtttgga tgagaactat accccgatac gaacatacca ggtgtgccaa gtcatggagc 301 ccaaccaaaa caactggctg cggactaact ggatttccaa aggcaatgca caaaggattt 361 ttgtagaatt gaaattcacc ctgagggatt gtaacagtct tcctggagta ctgggaactt 421 gcaaggaaac atttaatttg tactattatg aaacagacta tgacactggC AGGAATGTAA 481 GAGAAAACCT CTATGTAAAA ATAGACACCA TTGCTGCAGA TGAAAGTTTT ACCCAAGGTG 541 ACCTTGGTGA AAGAAAGATG AAGCTTAACA CTGAGGTGAG AGAGATTGGA CCTTTGTCCA 601 AAAAGGGATT CTATCTTGCC TTTCAGGATG TAGGGGCTTG CATAGCTTTG GTTTCTGTCA 661 AAGTGTACTA CAAGAAGTGC TGGTCCATTA TTGAGAACTT AGCTATCTTT CCAGATACAG 721 TGACTGGTTC AGAATTTTCC TCTTTAGTCG AGGTTCGAGG GACATGTGTC AGCAGTGCAG 781 AGGAAGAAGC GGAAAACGCC CCCAGGATGC ACTGCAGTGC AGAAGGAGAA TGGTTAGTGC 841 CCATTGGAAA ATGTATCTGC AAAGCAGGCT ACCAGCAAAA AGGAGACACT TGTGAATGTA 901 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 961 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1021 GGCTTTATAT ATCTTGTGGA AAGGACGAAA AGGCACATGG AGATCTATAT TTACGCGTTA 1081 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt