Transcript: Human NM_001288630.1

Homo sapiens EPH receptor A7 (EPHA7), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-21
Taxon:
Homo sapiens (human)
Gene:
EPHA7 (2045)
Length:
1935
CDS:
242..1081

Additional Resources:

NCBI RefSeq record:
NM_001288630.1
NBCI Gene record:
EPHA7 (2045)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146678 CACCTGGTATGTTCGTATCG pXPR_003 GGG 202 24% 3 0.0356 EPHA7 EPHA7 77353
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001288630.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196517 GTACTACTGCTGGATTCTAAA pLKO.1 338 CDS 100% 13.200 10.560 N EPHA7 n/a
2 TRCN0000006421 TGGTCCATTATTGAGAACTTA pLKO.1 857 CDS 100% 5.625 3.938 N EPHA7 n/a
3 TRCN0000349550 TGGTCCATTATTGAGAACTTA pLKO_005 857 CDS 100% 5.625 3.938 N EPHA7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001288630.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10803 pDONR223 100% 99.7% 99.2% None 412A>G;832C>T n/a
2 ccsbBroad304_10803 pLX_304 0% 99.7% 99.2% V5 412A>G;832C>T n/a
3 TRCN0000468569 AAAGGCACATGGAGATCTATATTT pLX_317 51.8% 99.7% 99.2% V5 412A>G;832C>T n/a
4 TRCN0000488943 CTGGTATCGTCGGTGTGCGGGGAA pLX_317 11.9% 28% 30.3% V5 (not translated due to prior stop codon) 832_833insCCT;834_835insGGCCGTGGGTTCTAC;837_838ins2127 n/a
5 TRCN0000489864 TTAGACTCGCTATCAGCCAGGGCC pLX_317 13.7% 28% 30.3% V5 (not translated due to prior stop codon) 832_833insCCT;834_835insGGCCGTGGGTTCTAC;837_838ins2128 n/a
6 ccsbBroadEn_06169 pDONR223 99.8% 27.9% 27.7% None 832_833insCCT;834_835insGGCCGTGGGTTCTAC;837_838ins2139 n/a
7 ccsbBroad304_06169 pLX_304 0% 27.9% 27.7% V5 832_833insCCT;834_835insGGCCGTGGGTTCTAC;837_838ins2139 n/a
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