Transcript: Mouse XM_017319955.1

PREDICTED: Mus musculus Eph receptor A7 (Epha7), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Epha7 (13841)
Length:
2671
CDS:
271..1875

Additional Resources:

NCBI RefSeq record:
XM_017319955.1
NBCI Gene record:
Epha7 (13841)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017319955.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000023680 CGGAAGTAACATTGGATACAT pLKO.1 1188 CDS 100% 5.625 7.875 N Epha7 n/a
2 TRCN0000023683 CCGGCAGGAATATACGAGAAA pLKO.1 443 CDS 100% 4.950 6.930 N Epha7 n/a
3 TRCN0000321890 CTTGCAAGGAAACGTTTAATT pLKO_005 395 CDS 100% 15.000 9.000 N Epha7 n/a
4 TRCN0000006420 CCAGTGAACAGAATCCTGTTA pLKO.1 1688 CDS 100% 0.495 0.347 N EPHA7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017319955.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488943 CTGGTATCGTCGGTGTGCGGGGAA pLX_317 11.9% 48.8% 56.3% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000489864 TTAGACTCGCTATCAGCCAGGGCC pLX_317 13.7% 48.8% 56.3% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_06169 pDONR223 99.8% 48.6% 51.5% None (many diffs) n/a
4 ccsbBroad304_06169 pLX_304 0% 48.6% 51.5% V5 (many diffs) n/a
5 ccsbBroadEn_10803 pDONR223 100% 31% 32.4% None (many diffs) n/a
6 ccsbBroad304_10803 pLX_304 0% 31% 32.4% V5 (many diffs) n/a
7 TRCN0000468569 AAAGGCACATGGAGATCTATATTT pLX_317 51.8% 31% 32.4% V5 (many diffs) n/a
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