Construct: ORF TRCN0000468610
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017423.1_s317c1
- Derived from:
- ccsbBroadEn_11281
- DNA Barcode:
- GGACTAGCCACGGGGCCGGGCTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKR7A2 (8574)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468610
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8574 | AKR7A2 | aldo-keto reductase family ... | NM_003689.3 | 91.9% | 91.9% | 1_87del |
| 2 | human | 246181 | AKR7L | aldo-keto reductase family ... | NM_001348421.1 | 91.7% | 88% | (many diffs) |
| 3 | human | 22977 | AKR7A3 | aldo-keto reductase family ... | NM_012067.3 | 90.5% | 86.5% | (many diffs) |
| 4 | human | 8574 | AKR7A2 | aldo-keto reductase family ... | NM_001320979.1 | 82.1% | 82.1% | 1_87del;484_485ins105 |
| 5 | human | 22977 | AKR7A3 | aldo-keto reductase family ... | XM_011541046.3 | 73.7% | 63.6% | (many diffs) |
| 6 | human | 8574 | AKR7A2 | aldo-keto reductase family ... | XR_946785.2 | 65.7% | 1_102del;605_606ins97;996_1262del | |
| 7 | human | 246182 | AKR7A2P1 | aldo-keto reductase family ... | NR_002796.2 | 59.4% | (many diffs) | |
| 8 | human | 22977 | AKR7A3 | aldo-keto reductase family ... | XM_017000714.2 | 58.1% | 49.8% | (many diffs) |
| 9 | human | 22977 | AKR7A3 | aldo-keto reductase family ... | XR_001737055.1 | 44.5% | (many diffs) | |
| 10 | human | 246181 | AKR7L | aldo-keto reductase family ... | NR_040288.2 | 38.3% | (many diffs) | |
| 11 | human | 246181 | AKR7L | aldo-keto reductase family ... | NR_040289.2 | 30.4% | (many diffs) | |
| 12 | mouse | 110198 | Akr7a5 | aldo-keto reductase family ... | NM_025337.3 | 79.1% | 81.7% | (many diffs) |
| 13 | mouse | 110198 | Akr7a5 | aldo-keto reductase family ... | XR_390721.3 | 51.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1056
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ccggccaccg ccaccgcggg tcgcctcggt gctgggcacc atggagatgg 121 ggcgccgcat ggacgcgccc gccagcgccg cggccgtgcg cgcctttctg gagcgcggcc 181 acaccgaact ggacacggcc ttcatgtaca gcgacggcca gtccgagacc atcctgggcg 241 gcctggggct cgggctgggc ggtggcgact gcagagtgaa aattgccacc aaggccaacc 301 cttgggatgg aaaatcacta aagcctgaca gtgtccggtc ccagctggag acgtcattga 361 agaggctgca gtgtccccaa gtggacctct tctacctaca cgcacctgac cacggcaccc 421 cggtggaaga gacgctgcat gcctgccagc ggctgcacca ggagggcaag ttcgtggagc 481 ttggcctctc caactatgct agctgggaag tggccgagat ctgtaccctc tgcaagagca 541 atggctggat cctgcccact gtgtaccagg gcatgtacaa cgccaccacc cggcaggtgg 601 aaacggagct cttcccctgc ctcaggcact ttggactgag gttctatgcc tacaaccctc 661 tggctggggg ccTGCTGACT GGCAAGTACA AGTATGAGGA CAAGGACGGG AAACAGCCTG 721 TGGGCCGCTT CTTTGGGAAT AGCTGGGCTG AGACCTACAG GAATCGCTTC TGGAAGGAGC 781 ACCACTTCGA GGCCATTGCG TTGGTGGAGA AGGCCCTGCA GGCCGCATAT GGCGCCAGCG 841 CCCCCAGTGT GACCTCGGCT GCCCTCCGGT GGATGTACCA CCACTCACAG CTGCAGGGTG 901 CCCACGGGGA CGCGGTCATC CTGGGCATGT CCAGCCTGGA GCAGCTGGAG CAGAACTTGG 961 CAGCAACAGA GGAAGGGCCC CTGGAGCCGG CTGTCGTGGA TGCCTTTAAT CAAGCCTGGC 1021 ATTTGGTTGC TCACGAATGT CCCAACTACT TCCGCTACCC AACTTTCTTG TACAAAGTGG 1081 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1141 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1201 AGGACTAGCC ACGGGGCCGG GCTGAACGCG TTAAGTCgac aatcaacctc tggattacaa 1261 aatttgtgaa agatt