Construct: ORF TRCN0000468617
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009752.1_s317c1
- Derived from:
- ccsbBroadEn_01091
- DNA Barcode:
- CCCGTGCAGTTGAGCGCTAGTGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFIB (4781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468617
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4781 | NFIB | nuclear factor I B | NM_005596.3 | 100% | 100% | |
| 2 | human | 4781 | NFIB | nuclear factor I B | NM_001369471.1 | 99.7% | 99.5% | 1057_1058insGTG |
| 3 | human | 4781 | NFIB | nuclear factor I B | NM_001369474.1 | 98.2% | 97.3% | (many diffs) |
| 4 | human | 4781 | NFIB | nuclear factor I B | NM_001369472.1 | 98.1% | 98.3% | (many diffs) |
| 5 | human | 4781 | NFIB | nuclear factor I B | NM_001369473.1 | 97.9% | 97.8% | (many diffs) |
| 6 | human | 4781 | NFIB | nuclear factor I B | NM_001369476.1 | 97.7% | 97.6% | 0_1ins28;2delT |
| 7 | human | 4781 | NFIB | nuclear factor I B | NM_001369477.1 | 95.4% | 95.2% | 560_561ins54;1003_1004insGTG |
| 8 | human | 4781 | NFIB | nuclear factor I B | NM_001190738.1 | 93.5% | 92.3% | (many diffs) |
| 9 | human | 4781 | NFIB | nuclear factor I B | NM_001369468.1 | 93.4% | 93.2% | (many diffs) |
| 10 | human | 4781 | NFIB | nuclear factor I B | NM_001369464.1 | 86.2% | 85.4% | 1244_1332del;1350_1461del |
| 11 | human | 4781 | NFIB | nuclear factor I B | NM_001369466.1 | 84.6% | 83.9% | (many diffs) |
| 12 | human | 4781 | NFIB | nuclear factor I B | NM_001190737.2 | 84.6% | 84.2% | (many diffs) |
| 13 | human | 4781 | NFIB | nuclear factor I B | NM_001369467.1 | 84.2% | 83.3% | (many diffs) |
| 14 | human | 4781 | NFIB | nuclear factor I B | XM_006716775.3 | 84% | 83.5% | (many diffs) |
| 15 | human | 4781 | NFIB | nuclear factor I B | XM_006716774.3 | 83.1% | 82.7% | (many diffs) |
| 16 | human | 4781 | NFIB | nuclear factor I B | NM_001369465.1 | 82.7% | 82.1% | (many diffs) |
| 17 | human | 4781 | NFIB | nuclear factor I B | NM_001369463.1 | 82.5% | 82.1% | (many diffs) |
| 18 | human | 4781 | NFIB | nuclear factor I B | NM_001369462.1 | 81.1% | 80.1% | (many diffs) |
| 19 | human | 4781 | NFIB | nuclear factor I B | NM_001369478.1 | 80.3% | 80.4% | (many diffs) |
| 20 | human | 4781 | NFIB | nuclear factor I B | NM_001369469.1 | 76.3% | 75.5% | 0_1ins144;1100_1188del;1206_1317del |
| 21 | human | 4781 | NFIB | nuclear factor I B | NM_001369461.1 | 74.5% | 74.1% | (many diffs) |
| 22 | human | 4781 | NFIB | nuclear factor I B | XM_005251467.3 | 73.4% | 72.9% | (many diffs) |
| 23 | human | 4781 | NFIB | nuclear factor I B | XM_006716773.3 | 73.2% | 72.6% | (many diffs) |
| 24 | human | 4781 | NFIB | nuclear factor I B | NM_001369460.1 | 72.1% | 71.7% | (many diffs) |
| 25 | human | 4781 | NFIB | nuclear factor I B | NM_001369475.1 | 70.8% | 70% | 336_337ins225;1019_1107del;1125_1236del |
| 26 | human | 4781 | NFIB | nuclear factor I B | NM_001369459.1 | 70.6% | 69.9% | (many diffs) |
| 27 | human | 4781 | NFIB | nuclear factor I B | NM_001369458.1 | 69.5% | 68.9% | (many diffs) |
| 28 | human | 4781 | NFIB | nuclear factor I B | NM_001369470.1 | 66.8% | 66.4% | (many diffs) |
| 29 | human | 4781 | NFIB | nuclear factor I B | NM_001369480.1 | 56.9% | 55.4% | (many diffs) |
| 30 | human | 4781 | NFIB | nuclear factor I B | NM_001369479.1 | 49.1% | 47% | (many diffs) |
| 31 | human | 4781 | NFIB | nuclear factor I B | NM_001369481.1 | 47.1% | 45.2% | (many diffs) |
| 32 | human | 4781 | NFIB | nuclear factor I B | NM_001282787.1 | 29.6% | 29.2% | (many diffs) |
| 33 | human | 4781 | NFIB | nuclear factor I B | XM_005251471.3 | 29.2% | 28.7% | (many diffs) |
| 34 | human | 4781 | NFIB | nuclear factor I B | XR_001746309.2 | 12.5% | (many diffs) | |
| 35 | human | 4781 | NFIB | nuclear factor I B | XR_001746308.2 | 12.5% | (many diffs) | |
| 36 | human | 4781 | NFIB | nuclear factor I B | NR_161383.1 | 12.2% | (many diffs) | |
| 37 | human | 4781 | NFIB | nuclear factor I B | NR_161385.1 | 9.9% | (many diffs) | |
| 38 | human | 4781 | NFIB | nuclear factor I B | NR_161382.1 | 9.9% | (many diffs) | |
| 39 | human | 4781 | NFIB | nuclear factor I B | NR_161384.1 | 9.7% | (many diffs) | |
| 40 | mouse | 18028 | Nfib | nuclear factor I/B | NM_008687.6 | 93.8% | 99.2% | (many diffs) |
| 41 | mouse | 18028 | Nfib | nuclear factor I/B | XM_017320021.1 | 90.8% | 95.6% | (many diffs) |
| 42 | mouse | 18028 | Nfib | nuclear factor I/B | XM_017320022.1 | 90.8% | 95.6% | (many diffs) |
| 43 | mouse | 18028 | Nfib | nuclear factor I/B | NM_001286131.1 | 80.9% | 84.8% | (many diffs) |
| 44 | mouse | 18028 | Nfib | nuclear factor I/B | NM_001286127.1 | 79.4% | 83.6% | (many diffs) |
| 45 | mouse | 18028 | Nfib | nuclear factor I/B | XM_006537654.2 | 78% | 82.1% | (many diffs) |
| 46 | mouse | 18028 | Nfib | nuclear factor I/B | NM_001113210.2 | 69.9% | 73.6% | (many diffs) |
| 47 | mouse | 18028 | Nfib | nuclear factor I/B | NM_001113209.2 | 68.8% | 72.4% | (many diffs) |
| 48 | mouse | 18028 | Nfib | nuclear factor I/B | XM_006537653.3 | 67.1% | 70.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1326
- ORF length:
- 1260
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gtattctccc atctgtctca ctcaggatga atttcaccca ttcatcgagg 121 cacttcttcc acatgtccgt gcaattgcct atacttggtt caacctgcag gctcgaaaac 181 gcaagtactt taaaaagcat gagaagcgaa tgtcaaagga tgaagaaaga gcagtcaaag 241 atgagcttct cagtgaaaag cctgaaatca aacagaagtg ggcatccagg ctccttgcca 301 aactgcgcaa agatattcgc caggagtatc gagaggactt tgtgctcacc gtgactggca 361 agaagcaccc gtgctgtgtc ttatccaatc ccgaccagaa gggtaagatt aggagaatcg 421 actgcctgcg acaggcagac aaagtctggc gtctggatct agtcatggtg atcctgttca 481 aaggcatccc cttggaaagt accgatggag agcggctcat gaaatcccca cattgcacaa 541 acccagcact ttgtgtccag ccacatcata tcacagtatc agttaaggag cttgatttgt 601 ttttggcata ctacgtgcag gagcaagatt ctggacaatc aggaagtcca agccacaatg 661 atcctgccaa gaatcctcca ggttaccttg aggatagttt tgtaaaatct ggagtcttca 721 atgtatcaga acttgtaaga gtatccagaa cgcccataac ccagggaact ggagtcaact 781 tcccaattgg agaaatccca agccaaccat actatcatga catgaactcg ggggtcaatc 841 ttcagaggtc tctgtcttct ccaccaagca gcaaaagacc caaaactata tccatagatg 901 aaaatatgga accaagtcct acaggagact tttaCCCCTC TCCAAGTTCA CCAGCTGCTG 961 GAAGTCGAAC ATGGCACGAA AGAGATCAAG ATATGTCTTC TCCGACTACT ATGAAGAAGC 1021 CTGAAAAGCC ATTGTTCAGC TCTGCATCTC CACAGGATTC TTCCCCAAGA CTGAGCACTT 1081 TCCCCCAGCA CCACCATCCC GGAATACCTG GAGTTGCACA CAGTGTCATC TCAACTCGAA 1141 CTCCACCTCC ACCTTCACCG TTGCCATTTC CAACACAAGC TATCCTTCCT CCAGCCCCAT 1201 CGAGCTACTT TTCTCATCCA ACAATCAGAT ATCCTCCCCA CCTGAATCCT CAGGATACTC 1261 TGAAGAACTA TGTACCTTCT TATGACCCAT CCAGTCCACA AACCAGCCAG TCCTGGTACC 1321 TGGGCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1381 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1441 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCGTGCAG TTGAGCGCTA GTGATACGCG 1501 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt