Construct: ORF TRCN0000468670

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010339.1_s317c1
Derived from:
ccsbBroadEn_11448
DNA Barcode:
GCAATACCGTGGGACACTGAGCCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PIGK (10026)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468670
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10026 PIGK phosphatidylinositol glycan... NM_005482.3 83.5% 83% (many diffs)
2 mouse 329777 Pigk phosphatidylinositol glycan... NM_025662.5 70% 76.5% (many diffs)
3 mouse 329777 Pigk phosphatidylinositol glycan... NM_178016.3 62.7% 68.2% (many diffs)
4 mouse 329777 Pigk phosphatidylinositol glycan... XM_006501612.3 53% 59.4% (many diffs)
5 mouse 329777 Pigk phosphatidylinositol glycan... XR_001783702.1 32.8% (many diffs)
6 mouse 329777 Pigk phosphatidylinositol glycan... XR_001783700.1 31% (many diffs)
7 mouse 329777 Pigk phosphatidylinositol glycan... XR_001783701.1 30.2% (many diffs)
8 mouse 329777 Pigk phosphatidylinositol glycan... XR_001783699.1 26.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1062
ORF length:
996
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cgtcaccgac agcctcagcc gggctgcgac tgtcttggca actgtgttgc
121tcttgtcctt cggcagcgtg gccgctagtc atatcgagga tcaagcagaa caattcttta
181gaagtggcca tacaaacaac tgggctgttc tggtgtgtac atcccgattc tggtttaatt
241atcgacatgt tgcaaatacc ctttctgttt atagaagtgt caagaggcta ggtattcctg
301acagtcacat tgtcctaatg cttgcagatg atatggcctg taatcctaga aatcccaaac
361cagctacagt gtttagtcac aagaatatgg aactaaatgt gtatggagat gatgtggaag
421tggattatag aagttatgag gtaactgtgg agaatttttt acgggtatta actgggggga
481tcccacctag tactcctcgg tcaaaacgtc ttctttctga tgacagaagc aatattctaa
541tttatatgac agggcatggt ggaaatggtt tcttaaaatt tcaagattct gaagaaatta
601ccaacataga actcgcggat gcttttgaac aaatgtggca gaaaagacgc tacaatgagc
661tactgtttat tattgatact tgccaaggag catccatgta tgaacgattt tattctccta
721acataatggc tctagctagt agtcaagtgG GAGAAGATTC ACTCTCGCAT CAACCTGATC
781CTGCAATTGG AGTCCATCTT ATGGATAGAT ACACATTTTA TGTCTTGGAA TTTTTGGAAG
841AAATTAACCC AGCTAGCCAA ACTAATATGA ATGACCTTTT TCAGGTATGT CCCAAAAGTC
901TGTGTGTGTC TACTCCTGGA CATCGCACTG ATCTTTTTCA GAGGGATCCT AAAAATGTAC
961TGATAACTGA TTTCTTTGGA AGTGTACGGA AAGTGGAAAT TACAACAGAG ACTATTAAAT
1021TGCAACAGGA TTCAGAAATC ATGGAAAGCA GGTATTCATC TTACCCAACT TTCTTGTACA
1081AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1141AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1201AGGACGAGCA ATACCGTGGG ACACTGAGCC TACGCGTTAA GTCgacaatc aacctctgga
1261ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)