Construct: ORF TRCN0000468673
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012406.1_s317c1
- Derived from:
- ccsbBroadEn_15939
- DNA Barcode:
- ATTCAATCCGCAACACTGGTATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHTF2 (57157)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468673
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366083.1 | 69.8% | 69.8% | 1_411del |
| 2 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366082.2 | 57.9% | 57.6% | (many diffs) |
| 3 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366088.2 | 57.9% | 57.6% | (many diffs) |
| 4 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_011516424.2 | 51.9% | 51.6% | 946_973del;976A>T;980_1830del |
| 5 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127360.1 | 46.3% | 43.3% | (many diffs) |
| 6 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127359.1 | 45.8% | 43% | (many diffs) |
| 7 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366081.1 | 45.5% | 45.3% | (many diffs) |
| 8 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366084.1 | 45% | 44.8% | (many diffs) |
| 9 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366087.1 | 45% | 44.8% | (many diffs) |
| 10 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_011516422.3 | 44.7% | 44.5% | (many diffs) |
| 11 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366085.2 | 44.6% | 44.3% | (many diffs) |
| 12 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127358.2 | 42.3% | 42.1% | (many diffs) |
| 13 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366086.1 | 42.3% | 42.1% | (many diffs) |
| 14 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_020432.5 | 42.3% | 42.1% | (many diffs) |
| 15 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127357.2 | 42.1% | 41.9% | (many diffs) |
| 16 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_005250508.5 | 42.1% | 41.9% | (many diffs) |
| 17 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_005250509.4 | 42.1% | 41.9% | (many diffs) |
| 18 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_006716063.4 | 41.9% | 40% | (many diffs) |
| 19 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366089.1 | 40.3% | 40.1% | (many diffs) |
| 20 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_017012459.2 | 32.6% | 32.3% | (many diffs) |
| 21 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_011516423.3 | 32.4% | 32.2% | (many diffs) |
| 22 | mouse | 68770 | Phtf2 | putative homeodomain transc... | XM_006535804.3 | 38.8% | 37.4% | (many diffs) |
| 23 | mouse | 68770 | Phtf2 | putative homeodomain transc... | NM_172992.3 | 37.8% | 37.6% | (many diffs) |
| 24 | mouse | 68770 | Phtf2 | putative homeodomain transc... | XM_006535801.1 | 28.8% | 28.5% | (many diffs) |
| 25 | mouse | 68770 | Phtf2 | putative homeodomain transc... | XM_006535803.1 | 22.2% | 16.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1017
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gctcctggga actgtgcatt gccagattgt ttccacaaga acacccaaac 121 ctcctctaag tacagggggt aaaagaagaa ggaaattaag aaaagcagcc catttggaag 181 tacataggga aggagatggt tctagtacca cagataacac acaagaggga gcagttcaga 241 accacggtac aagcacctct cacagcgttg gcactgtctt cagagatctc tggcatgctg 301 ctttcttttt atcaggatca aagaaagcaa agaattcaat tgataaatca actgaaactg 361 acaatggcta tgtatccctt gatgggaaga agactgttaa aagcggtgaa gatggaatac 421 aaaaccatga acctcagtgt gaaactattc gaccagaaga gacagcctgg aacacaggaa 481 cactgaggaa tggtcctagc aaagataccc aaaggacaat aacaaatgtc tctgatgaag 541 tctccagtga ggaaggtccT GAAACAGGAT ACTCATTACG TCGTCATGTG GACAGGACTT 601 CTGAAGGTGT TCTTCGGAAT AGAAAGTCAC ACCATTATAA GAAACATTAC CCTAATGAGG 661 ACGCCCCTAA ATCGGGTACT AGTTGCAGCT CTCGCTGTTC AAGTTCCAGA CAGGATTCTG 721 AGAGTGCAAG GCCAGAATCT GAAACAGAAG ATGTGTTATG GGAAGACTTG TTACATTGTG 781 CAGAATGCCA TTCATCTTGT ACCAGTGAGA CAGATGTGGA AAATCATCAG ATTAATCCAT 841 GTGTGAAAAA AGAATATAGA GATGACCCTT TTCATCAGAG TCATTTGCCC TGGCTCCATA 901 GTTCCCACCC AGGATTAGAA AAAATAAGTG CTATAGTATG GGAAGGTAAT GATTGTAAGA 961 AAGCAGACAT GTCTGTACTT GAAATCAGTG GAATGATAAT GAACAGAGTG AGTTTTTACC 1021 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1081 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1141 ATATATCTTG TGGAAAGGAC GAATTCAATC CGCAACACTG GTATCCACGC GTTAAGTCga 1201 caatcaacct ctggattaca aaatttgtga aagatt