Construct: ORF TRCN0000468730

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002095.1_s317c1
Derived from:
ccsbBroadEn_11804
DNA Barcode:
GATCTCCAGAATTACCCCTGCCCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DNM3 (26052)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468730
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26052 DNM3 dynamin 3 NM_001278252.2 99.9% 100% 243C>T
2 human 26052 DNM3 dynamin 3 NM_001350205.2 98.1% 98.2% 243C>T;1546_1575del
3 human 26052 DNM3 dynamin 3 XM_017000989.1 97.5% 97.3% (many diffs)
4 human 26052 DNM3 dynamin 3 XM_017000990.2 96.1% 90.1% 243C>T;1546_1591del;1692_1693insTAAAGATGATGAGAACATT
5 human 26052 DNM3 dynamin 3 XM_017000991.1 96.1% 90.1% 243C>T;1546_1591del;1692_1693insTAAAGATGATGAGAACATT
6 human 26052 DNM3 dynamin 3 XM_017000988.1 93.4% 93.2% (many diffs)
7 human 26052 DNM3 dynamin 3 XM_017000992.1 83.1% 77.7% (many diffs)
8 human 26052 DNM3 dynamin 3 XR_001737107.1 81.1% (many diffs)
9 human 26052 DNM3 dynamin 3 XM_017000984.1 65.3% 65.2% (many diffs)
10 human 26052 DNM3 dynamin 3 XM_017000985.1 65.3% 65.2% (many diffs)
11 human 26052 DNM3 dynamin 3 XM_017000983.1 65% 64.9% (many diffs)
12 human 26052 DNM3 dynamin 3 NM_001350206.2 64.5% 64.4% (many diffs)
13 human 26052 DNM3 dynamin 3 XM_017000982.2 64.5% 64.4% (many diffs)
14 human 26052 DNM3 dynamin 3 NM_001136127.3 64.4% 64.3% (many diffs)
15 human 26052 DNM3 dynamin 3 NM_015569.5 64.1% 64% (many diffs)
16 human 26052 DNM3 dynamin 3 NM_001350204.2 63.7% 63.6% (many diffs)
17 human 26052 DNM3 dynamin 3 XM_005245079.1 63.4% 63.3% (many diffs)
18 human 26052 DNM3 dynamin 3 XM_017000979.1 60.8% 60.8% (many diffs)
19 human 26052 DNM3 dynamin 3 XM_017000978.1 60.7% 60.7% (many diffs)
20 human 26052 DNM3 dynamin 3 XM_017000977.1 60.3% 60.3% (many diffs)
21 human 26052 DNM3 dynamin 3 XM_017000976.1 60.1% 60% (many diffs)
22 human 26052 DNM3 dynamin 3 XM_017000986.1 59.2% 58.5% (many diffs)
23 human 26052 DNM3 dynamin 3 XM_017000980.1 55.1% 54.5% (many diffs)
24 human 26052 DNM3 dynamin 3 XM_017000987.1 50.8% 50.7% (many diffs)
25 human 26052 DNM3 dynamin 3 XR_001737110.1 22% (many diffs)
26 human 26052 DNM3 dynamin 3 NR_146559.2 21.6% (many diffs)
27 human 26052 DNM3 dynamin 3 XR_001737108.1 21.6% (many diffs)
28 human 26052 DNM3 dynamin 3 XR_001737111.1 20.3% (many diffs)
29 mouse 103967 Dnm3 dynamin 3 XM_006496605.3 85.3% 90.2% (many diffs)
30 mouse 103967 Dnm3 dynamin 3 NM_172646.2 58.2% 63.4% (many diffs)
31 mouse 103967 Dnm3 dynamin 3 XM_006496603.3 58% 63.2% (many diffs)
32 mouse 103967 Dnm3 dynamin 3 XM_006496604.3 58% 63.2% (many diffs)
33 mouse 103967 Dnm3 dynamin 3 NM_001038619.1 57.9% 63.1% (many diffs)
34 mouse 103967 Dnm3 dynamin 3 XM_006496602.3 57.5% 62.7% (many diffs)
35 mouse 103967 Dnm3 dynamin 3 XM_006496601.3 57.3% 62.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1731
ORF length:
1665
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg gaaccgggag atggaggagc tgatcccgct ggtgaaccgt ctgcaggacg
121cgttttcggc gctgggacag agctgcctgc tggagctgcc gcagatcgcc gtggtgggcg
181gccagagcgc cggcaagagc tcggtgctcg agaacttcgt gggcagggac tttctccctc
241gagggtcggg cattgtaaca agacgacctc ttgtgctgca gcttgttact tctaaagcag
301aatatgctga gtttctacat tgcaaaggaa agaaatttac agattttgat gaagttcgcc
361ttgagattga agcagaaaca gatcgcgtga ctggaatgaa taaaggcatt tcctccatac
421ccattaattt acgagtctat tccccacacg tgttaaatct aacccttatt gatctacctg
481gaataactaa agtgcctgtg ggagatcagc caccagatat cgagtatcag atcagagaaa
541tgattatgca gttcatcacg agggagaact gtctgatttt agctgttact ccagccaaca
601ctgatcttgc aaactcagat gcgctgaagc tagctaaaga agttgatcct caaggtctga
661gaaccattgg agttatcacc aaactggacc ttatggatga aggaacggat gccagggatg
721ttctagagaa caaactgttg cctcttcgca ggggttacgt gggggtggta aacagaagcc
781agaaggacat agatgggaag aaggacataa aggcagccat gctggcagag aggaagtttt
841tcctttccca cccggcttac agacatatcg ctgaccgaat gggaacccca cacctgcaga
901aggtccttaa tcagcaactt accaaccaca ttcgggatac cctaccaaac ttcaggaaca
961aactacaggg acagttgctc tccatagaac atgaagtaga agcctacaaa aatttcaaac
1021cagaagaccc aacaaggaag accaaagcat tgctgcagat ggttcagcaa tttgctgtgg
1081actttgagaa gagaattgaa gggtcagggg atcaagtaga taccctggaa ctctcaggtg
1141gtgctaaaat caatcgtatt tttcatgaac gctttccttt tgagatagta aagatggagt
1201tcaatgagaa agaattgcga agagaaataa gctatgcaat caaaaacata catggtatca
1261ggacagggtt gtttactcca gacatggcat ttgaagcgat agtcaagaaa cagattgtaa
1321agttgaaagg gccttccttG AAGAGTGTGG ATCTGGTAAT ACAAGAATTA ATCAACACTG
1381TGAAGAAGTG TACCAAAAAA CTGGCAAACT TCCCCAGACT CTGCGAGGAA ACGGAAAGGA
1441TTGTTGCTAA CCACATTCGT GAGCGAGAAG GGAAGACAAA GGACCAGGTA TTGCTATTGA
1501TTGACATTCA AGTCTCTTAC ATCAACACCA ACCATGAAGA CTTCATTGGC TTCGCAAATG
1561CTCAGCAGAG GAGCAGTCAG GTTCACAAGA AAACCACAGT TGGAAATCAG GTGATTCGCA
1621AGGGGTGGCT CACCATCAGC AACATTGGCA TCATGAAAGG CGGCTCGAAG GGATACTGGT
1681TCGTCCTTAC TGCGGAAAGC TTGTCCTGGT ATAAAGATGA TGAGAACATT TACCCAACTT
1741TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1801CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1861CTTGTGGAAA GGACGAGATC TCCAGAATTA CCCCTGCCCC ACGCGTTAAG TCgacaatca
1921acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)