Construct: ORF TRCN0000468797
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018873.1_s317c1
- Derived from:
- ccsbBroadEn_14861
- DNA Barcode:
- GAGGTCCGTGGCCTGTTCCTGGGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TGFBR1 (7046)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468797
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7046 | TGFBR1 | transforming growth factor ... | NM_004612.4 | 98.9% | 43.4% | (many diffs) |
| 2 | human | 7046 | TGFBR1 | transforming growth factor ... | NM_001306210.2 | 98.1% | 43.1% | (many diffs) |
| 3 | human | 7046 | TGFBR1 | transforming growth factor ... | XM_011518949.2 | 85.3% | 30% | (many diffs) |
| 4 | human | 7046 | TGFBR1 | transforming growth factor ... | XM_024447658.1 | 85.3% | 30% | (many diffs) |
| 5 | human | 7046 | TGFBR1 | transforming growth factor ... | XM_011518948.2 | 84.6% | 29.8% | (many diffs) |
| 6 | human | 7046 | TGFBR1 | transforming growth factor ... | XM_017015063.1 | 84.6% | 29.8% | (many diffs) |
| 7 | human | 7046 | TGFBR1 | transforming growth factor ... | NM_001130916.3 | 83.6% | 28.2% | (many diffs) |
| 8 | human | 7046 | TGFBR1 | transforming growth factor ... | XM_011518950.2 | 70% | 14.8% | (many diffs) |
| 9 | mouse | 21812 | Tgfbr1 | transforming growth factor,... | NM_001312868.1 | 89.5% | 40.1% | (many diffs) |
| 10 | mouse | 21812 | Tgfbr1 | transforming growth factor,... | NM_009370.3 | 88.8% | 39.8% | (many diffs) |
| 11 | mouse | 21812 | Tgfbr1 | transforming growth factor,... | XM_017320103.1 | 77.5% | 28.8% | (many diffs) |
| 12 | mouse | 21812 | Tgfbr1 | transforming growth factor,... | NM_001312869.1 | 74.3% | 19.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 68
- ORF end:
- 800
- ORF length:
- 732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcccatg gaggcggcgg tcgctgctcc gcgtccccgg ctgctcctcc tcgtgctggc 121 ggcggtggcg gcggcggcgg cggcgctgct cccgggggcg acggcgttac agtgtttctg 181 ccacctctgt acaaaagaca attttacttg tgtgacagat gggctctgct ttgtctctgt 241 cacagagacc acagacaaag ttatacacaa cagcatgtgt atagctgaaa ttgacttaat 301 tcctcgagat aggccgtttg tatgtgcacc ctcttcaaaa actgggtctg tgactacaac 361 atattgctgc aatcaggacc attgcaataa aatagaactt ccaactactg taaagtcatc 421 acctggcctt ggtcctgtgg aactggcagc tgtcattgct ggaccagtgt gcttcgtctg 481 catctcactc atgttgatgg tctatatctg ccacaaccgc actgtcattc accatcgagt 541 gccaaatgaa gaggaccctt cattagatcg cccttttatt tcagagggta ctacgttgaa 601 agacttaatt tatgatatga caacgtcagg ttctggctca ggtttaccat tgcttgttca 661 gagaacaatt gcgagaacta ttgtgttaca agaaagcatt ggcaaagtcg atttggagaa 721 gtttggagag gaaagtggcg gggagaagaa gttgctgtta agatattctc ctctagagaa 781 gnacgttngg ttgntnccgt gaggcagaga ttttatcaaa ntgtaaatgt tacgtcatga 841 aaacatcctg gggattnnta gcagcagaca taaaagacaa tggtacttgg actcagctct 901 ggttggtgtc agattatcat gagcatggat ccctttttga ttacttaaac agatacacag 961 ttactgtgga aggaatgata aaacttgctc tgtccacggc gagcggtctt gcccatcttc 1021 acatggagat tgttggtacc caaggaaagc cagccattgc tcatagagat ttgaaatcaa 1081 agaatatctt ggtaaagaag aatggaactt gctgtattgc agacttagga ctggcagtaa 1141 gacatgattc agccacagat accattgata ttgctccaaa ccacagagtg ggaaCAAAAA 1201 GGTACATGGC CCCTGAAGTT CTCGATGATT CCATAAATAT GAAACATTTT GAATCCTTCA 1261 AACGTGCTGA CATCTATGCA ATGGGCTTAG TATTCTGGGA AATTGCTCGA CGATGTTCCA 1321 TTGGTGGAAT TCATGAAGAT TACCAACTGC CTTATTATGA TCTTGTACCT TCTGACCCAT 1381 CAGTTGAAGA AATGAGAAAA GTTGTTTGTG AACAGAAGTT AAGGCCAAAT ATCCCAAACA 1441 GATGGCAGAG CTGTGAAGCC TTGAGAGTAA TGGCTAAAAT TATGAGAGAA TGTTGGTATG 1501 CCAATGGAGC AGCTAGGCTT ACAGCATTGC GGATTAAGAA AACATTATCG CAACTCAGTC 1561 AACAGGAAGG CATCAAAATG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1621 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1681 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGAGG TCCGTGGCCT 1741 GTTCCTGGGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt