Construct: ORF TRCN0000468797

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018873.1_s317c1
Derived from:
ccsbBroadEn_14861
DNA Barcode:
GAGGTCCGTGGCCTGTTCCTGGGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
TGFBR1 (7046)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468797
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7046 TGFBR1 transforming growth factor ... NM_004612.4 98.9% 43.4% (many diffs)
2 human 7046 TGFBR1 transforming growth factor ... NM_001306210.2 98.1% 43.1% (many diffs)
3 human 7046 TGFBR1 transforming growth factor ... XM_011518949.2 85.3% 30% (many diffs)
4 human 7046 TGFBR1 transforming growth factor ... XM_024447658.1 85.3% 30% (many diffs)
5 human 7046 TGFBR1 transforming growth factor ... XM_011518948.2 84.6% 29.8% (many diffs)
6 human 7046 TGFBR1 transforming growth factor ... XM_017015063.1 84.6% 29.8% (many diffs)
7 human 7046 TGFBR1 transforming growth factor ... NM_001130916.3 83.6% 28.2% (many diffs)
8 human 7046 TGFBR1 transforming growth factor ... XM_011518950.2 70% 14.8% (many diffs)
9 mouse 21812 Tgfbr1 transforming growth factor,... NM_001312868.1 89.5% 40.1% (many diffs)
10 mouse 21812 Tgfbr1 transforming growth factor,... NM_009370.3 88.8% 39.8% (many diffs)
11 mouse 21812 Tgfbr1 transforming growth factor,... XM_017320103.1 77.5% 28.8% (many diffs)
12 mouse 21812 Tgfbr1 transforming growth factor,... NM_001312869.1 74.3% 19.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
68
ORF end:
800
ORF length:
732
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcccatg gaggcggcgg tcgctgctcc gcgtccccgg ctgctcctcc tcgtgctggc
121ggcggtggcg gcggcggcgg cggcgctgct cccgggggcg acggcgttac agtgtttctg
181ccacctctgt acaaaagaca attttacttg tgtgacagat gggctctgct ttgtctctgt
241cacagagacc acagacaaag ttatacacaa cagcatgtgt atagctgaaa ttgacttaat
301tcctcgagat aggccgtttg tatgtgcacc ctcttcaaaa actgggtctg tgactacaac
361atattgctgc aatcaggacc attgcaataa aatagaactt ccaactactg taaagtcatc
421acctggcctt ggtcctgtgg aactggcagc tgtcattgct ggaccagtgt gcttcgtctg
481catctcactc atgttgatgg tctatatctg ccacaaccgc actgtcattc accatcgagt
541gccaaatgaa gaggaccctt cattagatcg cccttttatt tcagagggta ctacgttgaa
601agacttaatt tatgatatga caacgtcagg ttctggctca ggtttaccat tgcttgttca
661gagaacaatt gcgagaacta ttgtgttaca agaaagcatt ggcaaagtcg atttggagaa
721gtttggagag gaaagtggcg gggagaagaa gttgctgtta agatattctc ctctagagaa
781gnacgttngg ttgntnccgt gaggcagaga ttttatcaaa ntgtaaatgt tacgtcatga
841aaacatcctg gggattnnta gcagcagaca taaaagacaa tggtacttgg actcagctct
901ggttggtgtc agattatcat gagcatggat ccctttttga ttacttaaac agatacacag
961ttactgtgga aggaatgata aaacttgctc tgtccacggc gagcggtctt gcccatcttc
1021acatggagat tgttggtacc caaggaaagc cagccattgc tcatagagat ttgaaatcaa
1081agaatatctt ggtaaagaag aatggaactt gctgtattgc agacttagga ctggcagtaa
1141gacatgattc agccacagat accattgata ttgctccaaa ccacagagtg ggaaCAAAAA
1201GGTACATGGC CCCTGAAGTT CTCGATGATT CCATAAATAT GAAACATTTT GAATCCTTCA
1261AACGTGCTGA CATCTATGCA ATGGGCTTAG TATTCTGGGA AATTGCTCGA CGATGTTCCA
1321TTGGTGGAAT TCATGAAGAT TACCAACTGC CTTATTATGA TCTTGTACCT TCTGACCCAT
1381CAGTTGAAGA AATGAGAAAA GTTGTTTGTG AACAGAAGTT AAGGCCAAAT ATCCCAAACA
1441GATGGCAGAG CTGTGAAGCC TTGAGAGTAA TGGCTAAAAT TATGAGAGAA TGTTGGTATG
1501CCAATGGAGC AGCTAGGCTT ACAGCATTGC GGATTAAGAA AACATTATCG CAACTCAGTC
1561AACAGGAAGG CATCAAAATG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1621CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1681AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGAGG TCCGTGGCCT
1741GTTCCTGGGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)