Construct: ORF TRCN0000468798
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018872.2_s317c1
- Derived from:
- ccsbBroadEn_15268
- DNA Barcode:
- TCTAGAATGAACCAGTCTGATATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NEK10 (152110)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468798
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152110 | NEK10 | NIMA related kinase 10 | NM_001031741.3 | 89.5% | 36.4% | (many diffs) |
| 2 | human | 152110 | NEK10 | NIMA related kinase 10 | NM_001304384.1 | 87.6% | 35.7% | (many diffs) |
| 3 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_024453374.1 | 58.2% | 23.1% | (many diffs) |
| 4 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005772.2 | 38.2% | 15% | (many diffs) |
| 5 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_006713001.3 | 37.2% | 14.7% | (many diffs) |
| 6 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005771.2 | 37.2% | 14.7% | (many diffs) |
| 7 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005769.1 | 37.2% | 14.6% | (many diffs) |
| 8 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005770.2 | 37.2% | 14.6% | (many diffs) |
| 9 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005768.1 | 37.2% | 14.6% | (many diffs) |
| 10 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005767.2 | 36.6% | 14.4% | (many diffs) |
| 11 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_024453373.1 | 36.6% | 14.4% | (many diffs) |
| 12 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_006712997.3 | 36.1% | 14.1% | (many diffs) |
| 13 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005766.2 | 36.1% | 14.1% | (many diffs) |
| 14 | human | 152110 | NEK10 | NIMA related kinase 10 | NM_152534.4 | 35.7% | 14% | (many diffs) |
| 15 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005761.2 | 35.7% | 14% | (many diffs) |
| 16 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005762.2 | 35.7% | 14% | (many diffs) |
| 17 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005763.1 | 35.7% | 14% | (many diffs) |
| 18 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005764.1 | 35.7% | 14% | (many diffs) |
| 19 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005765.1 | 35.7% | 14% | (many diffs) |
| 20 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_006712998.2 | 34.7% | 14.1% | (many diffs) |
| 21 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_006712999.3 | 34.5% | 14.2% | (many diffs) |
| 22 | human | 152110 | NEK10 | NIMA related kinase 10 | XM_017005773.1 | 22% | 17.4% | (many diffs) |
| 23 | human | 152110 | NEK10 | NIMA related kinase 10 | XR_001740034.1 | 19.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 624
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggttcctgta cagcctgcag aaccccccga ggtactgaag agtgagccgt 121 atggggagaa ggctgatgtc tgggcagtag gctgcatcct ttatcagatg gcgactttga 181 gtcccccctt ctacagcact aacatgctgt ccttggctac aaaaatagtg gaggcggtat 241 atgaaccagt gccagaaggt atctactctg aaaaagtaac agacaccatc agcaggtgcc 301 tcactcctga tgcggaagct cgtccagata ttgtagaagt cagttcgatg atatcagatg 361 tcatgatgaa atatttagac aacttatcta catcccagtt gtccttggaa aagaagctag 421 aacgggaacg aagacgcaca caaaggtatt ttatggaagc caaccggaac accgtcacat 481 gtcaccatga gctggctgtt ctatctcacg agacctttga gaaggcaagt ttgagtagca 541 ncagcnnngg ancagccanc ctgaaaantg aactttcana aagcgcagac ctgccccctg 601 naggctttcc aggcctccta tggtaaagac gaagacaggg cctgtgacga aatcctgtca 661 gatgataact tcaacctgga aaatgctgag aaagatacat attcagaggt agatgatgaa 721 ttggacattt cggataactc cagcagctcc agttcaagcc ctctgaaagn atctacattc 781 aacattttaa agagaagttt tagtgcttca ggaggagaaa gacannccca aannagggac 841 ttcactggag gaacaggatc aagaccaaga ccagacagac agcatcagca ggaattgctg 901 tgtcccagag gaaagtgcgt cagatcagtg atcctattca gcagatatta attcagctgc 961 acaaaataat ctatatcaca cagcttcctc cagctttgca ccacaatttg aaaagaaggg 1021 ttatagagag attcaagaaa TCCCTCTTCA GCCAGCAGAG TAACCCTTGT AATTTGAAAT 1081 CTGAAATTAA AAAGTTATCT CAGGGATCTC CAGAACCGAT TGAGCCCAAC TTTTTCACAG 1141 CAGATTACCA TTTATTACAT CGTTCATCCG GTGGAAACAG CCTGTCCCCA AATGACCCTA 1201 CAGGTTTACC AACCAGCATT GAATTGGAGG AAGGAATAAC ATATGAACAG ATGCAGACTG 1261 TGATTGAAGA AGTCCTTGAG GAAAGTGGCT ATTACAATTT TACATCTAAC AGGTATCATT 1321 CCTATCCATG GGGGACCAAG AATCACCCAA CCAAAAGATT GCCAACTTTC TTGTACAAAG 1381 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1441 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1501 ACGATCTAGA ATGAACCAGT CTGATATCAC GCGTTAAGTC gacaatcaac ctctggatta 1561 caaaatttgt gaaagatt