Construct: ORF TRCN0000468931
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012667.1_s317c1
- Derived from:
- ccsbBroadEn_10410
- DNA Barcode:
- TTGATACAAGTTGGTTCCTGGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GUSBP9 (100049076)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468931
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375513 | GUSBP4 | GUSB pseudogene 4 | NR_133000.1 | 48.6% | (many diffs) | |
| 2 | human | 100049076 | GUSBP9 | GUSB pseudogene 9 | NR_033968.1 | 44.2% | 1_295del;566_610del | |
| 3 | human | 106660613 | LINC00680-GUSBP4 | LINC00680-GUSBP4 readthrough | NR_132998.1 | 37% | (many diffs) | |
| 4 | human | 728411 | GUSBP1 | GUSB pseudogene 1 | NR_027028.2 | 35.5% | (many diffs) | |
| 5 | human | 106660613 | LINC00680-GUSBP4 | LINC00680-GUSBP4 readthrough | NR_003660.2 | 33.5% | (many diffs) | |
| 6 | human | 102725009 | LOC102725009 | putative inactive beta-gluc... | XM_017030249.2 | 33.4% | 13.8% | (many diffs) |
| 7 | human | 11039 | GUSBP14 | GUSB pseudogene 14 | NR_024054.2 | 25.8% | (many diffs) | |
| 8 | human | 106660613 | LINC00680-GUSBP4 | LINC00680-GUSBP4 readthrough | NR_132997.1 | 25.1% | (many diffs) | |
| 9 | human | 387036 | GUSBP2 | GUSB pseudogene 2 | NR_003504.3 | 21.7% | (many diffs) | |
| 10 | human | 2990 | GUSB | glucuronidase beta | XM_017012093.2 | 21.2% | 15.3% | (many diffs) |
| 11 | human | 2990 | GUSB | glucuronidase beta | XM_017012092.1 | 19.4% | 14% | (many diffs) |
| 12 | human | 2990 | GUSB | glucuronidase beta | XM_011516114.2 | 18.7% | 13.5% | (many diffs) |
| 13 | human | 2990 | GUSB | glucuronidase beta | NM_001293105.2 | 18.4% | 13.3% | (many diffs) |
| 14 | human | 2990 | GUSB | glucuronidase beta | XM_017012091.1 | 18.4% | 13.3% | (many diffs) |
| 15 | human | 2990 | GUSB | glucuronidase beta | NM_001293104.2 | 17.3% | 12.5% | (many diffs) |
| 16 | human | 653188 | GUSBP3 | GUSB pseudogene 3 | NR_027386.2 | 17.2% | (many diffs) | |
| 17 | human | 2990 | GUSB | glucuronidase beta | XM_011516113.1 | 16.5% | 11.9% | (many diffs) |
| 18 | human | 2990 | GUSB | glucuronidase beta | NM_001284290.2 | 15.8% | 11.4% | (many diffs) |
| 19 | human | 11042 | GUSBP15 | GUSB pseudogene 15 | NR_034021.1 | 15% | (many diffs) | |
| 20 | human | 11039 | GUSBP14 | GUSB pseudogene 14 | NR_029426.1 | 13.5% | (many diffs) | |
| 21 | human | 2990 | GUSB | glucuronidase beta | XM_005250297.4 | 13.3% | 9.6% | (many diffs) |
| 22 | human | 2990 | GUSB | glucuronidase beta | NM_000181.4 | 12.2% | 8.8% | (many diffs) |
| 23 | human | 2990 | GUSB | glucuronidase beta | XR_001744660.2 | 11.9% | (many diffs) | |
| 24 | human | 2990 | GUSB | glucuronidase beta | XR_927461.3 | 11% | (many diffs) | |
| 25 | human | 653080 | GUSBP16 | GUSB pseudogene 16 | NR_146391.1 | 11% | (many diffs) | |
| 26 | human | 11039 | GUSBP14 | GUSB pseudogene 14 | XR_002958912.1 | 5.2% | (many diffs) | |
| 27 | human | 375513 | GUSBP4 | GUSB pseudogene 4 | NR_132999.1 | 5.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 123
- ORF end:
- 393
- ORF length:
- 270
- Sequence:
-
1 TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC 61 ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG TTTGTACAAA AAAGTTGGCA 121 CCATGGTGAT TGCTCACACC AAAGCCTTGG ACCCCTCCCA GCCTGTGACC TTTGGGACCA 181 ACTCCACCTA CGCAGCAGAC AAGGGGGCTC TGTATGTGGA TGTGATCCGT GTGAACAGCT 241 ACTACTCTTG GTATCGCAAC TACGGGCACC TGGAGTTGAT TCGGCTGCAG CTGGCCGCCC 301 AGTTTGAGAA TTGGTGTAAG ACATCACAAT CCCATTATTC AGAGCGCGTA TGGAGTGGAA 361 ACGCTTGTAG GGCTTCACCA GGTAAGCGGT GTTTGCCAAC TTTCTTGTAC AAAGTGGTTG 421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 541 GATACAAGTT GGTTCCTGGC AGACGCGTTA AGTCgacaat caacctctgg attacaaaat 601 ttgtgaaaga tt