Construct: ORF TRCN0000469004
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018931.3_s317c1
- Derived from:
- ccsbBroadEn_14780
- DNA Barcode:
- AGCTCTTGCTTGTTTAGCGGTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKACA (5566)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469004
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5566 | PRKACA | protein kinase cAMP-activat... | NM_002730.4 | 99.7% | 99.4% | 680T>N;682_683delCTinsNN |
| 2 | human | 5566 | PRKACA | protein kinase cAMP-activat... | NM_207518.3 | 96.5% | 95.4% | (many diffs) |
| 3 | human | 5568 | PRKACG | protein kinase cAMP-activat... | NM_002732.3 | 85.1% | 82.3% | (many diffs) |
| 4 | human | 5566 | PRKACA | protein kinase cAMP-activat... | XM_017026948.1 | 84% | 83.7% | 680T>N;682_683delCTinsNN;760_761ins165 |
| 5 | human | 5566 | PRKACA | protein kinase cAMP-activat... | NM_001304349.1 | 81.2% | 78.9% | (many diffs) |
| 6 | human | 5567 | PRKACB | protein kinase cAMP-activat... | XM_006710758.2 | 73.5% | 87.9% | (many diffs) |
| 7 | human | 5567 | PRKACB | protein kinase cAMP-activat... | NM_207578.3 | 55.3% | 67.5% | (many diffs) |
| 8 | human | 5567 | PRKACB | protein kinase cAMP-activat... | XM_017001713.2 | 55.3% | 67.5% | (many diffs) |
| 9 | mouse | 18747 | Prkaca | protein kinase, cAMP depend... | NM_008854.5 | 90.9% | 97.4% | (many diffs) |
| 10 | mouse | 18747 | Prkaca | protein kinase, cAMP depend... | NM_001277898.1 | 88.1% | 93.4% | (many diffs) |
| 11 | mouse | 18749 | Prkacb | protein kinase, cAMP depend... | NM_011100.4 | 78.1% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1122
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcaacgcc gccgccgcca agaagggcag cgagcaggag agcgtgaaag 121 aattcttagc caaagccaaa gaagattttc ttaaaaaatg ggaaagtccc gctcagaaca 181 cagcccactt ggatcagttt gaacgaatca agaccctcgg cacgggctcc ttcgggcggg 241 tgatgctggt gaaacacaag gagaccggga accactatgc catgaagatc ctcgacaaac 301 agaaggtggt gaaactgaaa cagatcgaac acaccctgaa tgaaaagcgc atcctgcaag 361 ctgtcaactt tccgttcctc gtcaaactcg agttctcctt caaggacaac tcaaacttat 421 acatggtcat ggagtacgtg cccggcgggg agatgttctc acacctacgg cggatcggaa 481 ggttcagtga gccccatgcc cgtttctacg cggcccagat cgtcctgacc tttgagtatc 541 tgcactcgct ggatctcatc tacagggacc tgaagccgga gaatctgctc attgaccagc 601 agggctacat tcaggtgaca gacttcggtt tcgccaagcg cgtgaagggc cgcacttgga 661 ccttgtgcgg cacccctgag tacctggccc ctgagattat cctgagcaaa ggctacaaca 721 aggccgtgga ctggtgggcc ctgggggntn ntatctatga aatggccgct ggctacccgc 781 ccTTCTTCGC AGACCAGCCC ATCCAGATCT ATGAGAAGAT CGTCTCTGGG AAGGTGCGCT 841 TCCCTTCCCA CTTCAGCTCT GACTTGAAGG ACCTGCTGCG GAACCTCCTG CAGGTAGATC 901 TCACCAAGCG CTTTGGGAAC CTCAAGAATG GGGTCAACGA TATCAAGAAC CACAAGTGGT 961 TTGCCACAAC TGACTGGATT GCCATCTACC AGAGGAAGGT GGAAGCTCCC TTCATACCAA 1021 AGTTTAAAGG CCCTGGGGAT ACGAGTAACT TTGACGACTA TGAGGAAGAA GAAATCCGGG 1081 TCTCCATCAA TGAGAAGTGT GGCAAGGAGT TTTCTGAGTT TTTGCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGAAGC TCTTGCTTGT TTAGCGGTAC GACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t