Construct: ORF TRCN0000469041
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010881.1_s317c1
- Derived from:
- ccsbBroadEn_14486
- DNA Barcode:
- ATCACAATTGGACGAATGGAGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERCC6L2 (375748)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469041
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375748 | ERCC6L2 | ERCC excision repair 6 like 2 | XM_011518650.3 | 54.6% | 54.5% | 1_1143del;1150T>C;1425G>T |
| 2 | human | 375748 | ERCC6L2 | ERCC excision repair 6 like 2 | XM_017014707.2 | 50.4% | 50.4% | 1_1350del;1357T>C;1632G>T |
| 3 | human | 375748 | ERCC6L2 | ERCC excision repair 6 like 2 | XM_011518648.3 | 45.6% | 45.6% | 1_1638del;1645T>C;1920G>T |
| 4 | human | 375748 | ERCC6L2 | ERCC excision repair 6 like 2 | XM_011518644.3 | 36.3% | 36.3% | 1_2409del;2416T>C;2691G>T |
| 5 | human | 375748 | ERCC6L2 | ERCC excision repair 6 like 2 | XM_011518641.3 | 30.4% | 30.3% | 1_3150del;3157T>C;3432G>T |
| 6 | human | 375748 | ERCC6L2 | ERCC excision repair 6 like 2 | NM_020207.5 | 29.4% | 29.4% | 1_3303del;3310T>C;3585G>T |
| 7 | human | 375748 | ERCC6L2 | ERCC excision repair 6 like 2 | XR_001746290.2 | 27.3% | (many diffs) | |
| 8 | human | 375748 | ERCC6L2 | ERCC excision repair 6 like 2 | XR_001746291.2 | 20.9% | (many diffs) | |
| 9 | human | 375748 | ERCC6L2 | ERCC excision repair 6 like 2 | XR_929789.2 | 17.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1446
- ORF length:
- 1380
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa acgttcaaat gagaaagttg ttaatcaaga gcagtcgtat gaatcaatgg 121 ataaattttt agatggcgtt caggaagtgg cttatattca ctcaaaccag aatgtaattg 181 gatcgagcaa agctgaaaat cacatgagcc gatgggcagc acatgacgta tttgagttga 241 agcagttttc tcagctgcct gctaacatag ctgtttgcag ttctaagaca tataaagaaa 301 aagtggatgc agatacattg ccacacacaa agaaaggcca gcaacctagt gaaggcagca 361 tttcacttcc tctttacatt tcaaatcctg taaaccagaa gaagaaaaaa gtctaccata 421 caaaccagac caccttcata attggagaaa caccaaaagg aatccgcaga aaacaatttg 481 aagaaatggc ctcttatttt aactcgtctt ctgtaaacga atttgctaaa catataacca 541 atgccacatc agaagaacga cagaaaatgc taagagactt ttatgcttct caatatccag 601 aggtaaaaga attttttgtg gattctgtgt cacaattcaa caattcttcc tttgagaaag 661 gagagcagcg cacccggaag aaatctgata aaagagaatc tcttataaaa ccaaggctgt 721 cagattctga aaccttgtca tttaaagatt ctaccaacaa aatttctcaa gtttgcagcc 781 taaaaacata taaaagaaaa tcagttaagt ttcagaatca tatttcctat agagaagagg 841 tgttttttaa tgatgcagaa actaagaaat cacctgttag ttctactcaa gagattgaca 901 gtgggaaaaa cagccaggca tccgaagata ctgtgacatc ccgttctctg aacagtgagt 961 ctgaaacacg tgagagaagg ttagaaaata ccatgaaaga CCAACAGGAC CTCACAAGAA 1021 CGGGCATTTC AAGAAAAGAA CCCCTTCTCA AATTGGAAAA CAAAAAGATA GAAAATCCAG 1081 TGCTGGAAAA TACTTCTGTG ATAAGCTTAC TTGGTGATAC CTCTATTCTT GATGACCTTT 1141 TTAAAAGTCA TGGGAACAGT CCCACACAAC TGCCAAAGAA AGTTCTTTCA GGGCCCATGG 1201 AAAAAGCAAA ACAGAGACCA AAAGATTTCT GGGACATCTT GAATGAGCAG AATGATGAGA 1261 GTCTTAGTAA ACTCACAGAC TTGGCAGTAA TAGAGACTCT GTGTGAAAAA GCACCTCTAG 1321 CAGCACCCTT TAAAAGGAGA GAAGAGCCAG CAACTTCTCT TTGGAAATCA AATGAGAAAT 1381 TTTTATGGAA GAAATTTAGC CCAAGTGATA CAGATGAAAA CGCAACCAAT ACACAGAGTA 1441 CCACATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1501 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1561 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCACAATT GGACGAATGG AGACGACGCG 1621 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt