Construct: ORF TRCN0000469084

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015546.1_s317c1
Derived from:
ccsbBroadEn_06378
DNA Barcode:
CCGCATGATACGGACCGACTTCAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HLA-F (3134)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469084
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3134 HLA-F major histocompatibility co... NM_018950.2 99.8% 99.7% 63G>A;814C>T
2 human 3134 HLA-F major histocompatibility co... XM_011514564.1 97.4% 96.5% (many diffs)
3 human 3134 HLA-F major histocompatibility co... XM_017010813.1 89% 88.6% (many diffs)
4 human 3135 HLA-G major histocompatibility co... NM_002127.5 83.5% 76.2% (many diffs)
5 human 3135 HLA-G major histocompatibility co... XM_024446420.1 83.5% 76.2% (many diffs)
6 human 3105 HLA-A major histocompatibility co... NM_002116.8 82.7% 76.1% (many diffs)
7 human 3135 HLA-G major histocompatibility co... NM_001363567.1 82.3% 75.1% (many diffs)
8 human 3134 HLA-F major histocompatibility co... XM_017010812.1 82.2% 81.9% (many diffs)
9 human 3106 HLA-B major histocompatibility co... NM_005514.8 81.8% 76.2% (many diffs)
10 human 3107 HLA-C major histocompatibility co... NM_001243042.1 81.8% 74% (many diffs)
11 human 3107 HLA-C major histocompatibility co... NM_002117.6 81.7% 74% (many diffs)
12 human 3105 HLA-A major histocompatibility co... NM_001242758.1 81.3% 75.6% (many diffs)
13 human 3134 HLA-F major histocompatibility co... XM_017010811.1 79.8% 76.8% (many diffs)
14 human 3134 HLA-F major histocompatibility co... XM_017010810.1 79.3% 79% (many diffs)
15 human 3134 HLA-F major histocompatibility co... NM_001098479.2 78.1% 77.8% (many diffs)
16 human 3134 HLA-F major histocompatibility co... NM_001098478.2 73.3% 73.4% 63G>A;609_610ins276
17 human 3133 HLA-E major histocompatibility co... XM_017010807.1 71.6% 58.4% (many diffs)
18 human 3133 HLA-E major histocompatibility co... XM_017010808.1 71.6% 58.4% (many diffs)
19 human 3134 HLA-F major histocompatibility co... XM_017010815.1 65.7% 65.6% 0_1ins354;460C>T
20 human 3135 HLA-G major histocompatibility co... XM_017010817.1 62.1% 56.7% (many diffs)
21 human 3135 HLA-G major histocompatibility co... XM_017010818.1 62.1% 56.7% (many diffs)
22 human 3136 HLA-H major histocompatibility co... NR_001434.4 53.1% (many diffs)
23 human 3134 HLA-F major histocompatibility co... XM_017010814.1 51.5% 51.1% (many diffs)
24 human 3134 HLA-F major histocompatibility co... XR_001743373.1 32% (many diffs)
25 human 3134 HLA-F major histocompatibility co... XR_001743374.1 30.7% (many diffs)
26 human 3134 HLA-F major histocompatibility co... XR_001743376.1 30.5% (many diffs)
27 human 3139 HLA-L major histocompatibility co... NR_027822.1 15.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1104
ORF length:
1038
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gccccgaagc ctcctcctgc tgctctcagg ggccctggcc ctgaccgata
121cttgggcagg ctcccactcc ttgaggtatt tcagcaccgc tgtgtcgcgg cccggccgcg
181gggagccccg ctacatcgcc gtggagtacg tagacgacac gcaattcctg cggttcgaca
241gcgacgccgc gattccgagg atggagccgc gggagccgtg ggtggagcaa gaggggccgc
301agtattggga gtggaccaca gggtacgcca aggccaacgc acagactgac cgagtggccc
361tgaggaacct gctccgccgc tacaaccaga gcgaggctgg gtctcacacc ctccagggaa
421tgaatggctg cgacatgggg cccgacggac gcctcctccg cgggtatcac cagcacgcgt
481acgacggcaa ggattacatc tccctgaacg aggacctgcg ctcctggacc gcggcggaca
541ccgtggctca gatcacccag cgcttctatg aggcagagga atatgcagag gagttcagga
601cctacctgga gggcgagtgc ctggagttgc tccgcagata cttggagaat gggaaggaga
661cgctacagcg cgcagatcct ccaaaggcac acgttgccca ccaccccatc tctgaccatg
721aggCCACCCT GAGGTGCTGG GCCCTGGGCT TCTACCCTGC GGAGATCACG CTGACCTGGC
781AGCGGGATGG GGAGGAACAG ACCCAGGACA CAGAGCTTGT GGAGACCAGG CCTGCAGGGG
841ATGGAACCTT CCAGAAGTGG GCCGCTGTGG TGGTGCCTTC TGGAGAGGAA CAGAGATACA
901CATGCCATGT GCAGCACGAG GGGCTGCCCC AGCCCCTCAT CCTGAGATGG GAGCAGTCTC
961CCCAGCCCAC CATCCCCATC GTGGGCATCG TTGCTGGCCT TGTTGTCCTT GGAGCTGTGG
1021TCACTGGAGC TGTGGTCGCT GCTGTGATGT GGAGGAAGAA GAGCTCAGAT AGAAACAGAG
1081GGAGCTACTC TCAGGCTGCA GTGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1141AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1201TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CGCATGATAC
1261GGACCGACTT CAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1321att

Download FASTA (ORF) (Full)