Construct: ORF TRCN0000469111
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019005.3_s317c1
- Derived from:
- ccsbBroadEn_14720
- DNA Barcode:
- CGAATCGAACTGAGCTTCCAGCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NTRK1 (4914)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469111
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4914 | NTRK1 | neurotrophic receptor tyros... | NM_001012331.1 | 100% | 100% | |
| 2 | human | 4914 | NTRK1 | neurotrophic receptor tyros... | NM_002529.3 | 99.2% | 99.2% | 1177_1194del |
| 3 | human | 4914 | NTRK1 | neurotrophic receptor tyros... | NM_001007792.1 | 94% | 89.3% | (many diffs) |
| 4 | mouse | 18211 | Ntrk1 | neurotrophic tyrosine kinas... | XM_006501124.2 | 84.7% | 86.4% | (many diffs) |
| 5 | mouse | 18211 | Ntrk1 | neurotrophic tyrosine kinas... | NM_001033124.1 | 84.1% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2439
- ORF length:
- 2370
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgcgaggc ggacggcgcg ggcagcttgg ctggcacagc tgggctgcgg 121 ggccgggcag cctgctggct tggctgatac tggcatctgc gggcgccgca ccctgccccg 181 atgcctgctg cccccacggc tcctcgggac tgcgatgcac ccgggatggg gccctggata 241 gcctccacca cctgcccggc gcagagaacc tgactgagct ctacatcgag aaccagcagc 301 atctgcagca tctggagctc cgtgatctga ggggcctggg ggagctgaga aacctcacca 361 tcgtgaagag tggtctccgt ttcgtggcgc cagatgcctt ccatttcact cctcggctca 421 gtcgcctgaa tctctccttc aacgctctgg agtctctctc ctggaaaact gtgcagggcc 481 tctccttaca ggaactggtc ctgtcgggga accctctgca ctgttcttgt gccctgcgct 541 ggctacagcg ctgggaggag gagggactgg gcggagtgcc tgaacagaag ctgcagtgtc 601 atgggcaagg gcccctggcc cacatgccca atgccagctg tggtgtgccc acgctgaagg 661 tccaggtgcc caatgcctcg gtggatgtgg gggacgacgt gctgctgcgg tgccaggtgg 721 aggggcgggg cctggagcag gccggctgga tcctcacaga gctggagcag tcagccacgg 781 tgatgaaatc tgggggtctg ccatccctgg ggctgaccct ggccaatgtc accagtgacc 841 tcaacaggaa gaacgtgacg tgctgggcag agaacgatgt gggccgggca gaggtctctg 901 ttcaggtcaa cgtctccttc ccggccagtg tgcagctgca cacggcggtg gagatgcacc 961 actggtgcat ccccttctct gtggatgggc agccggcacc gtctctgcgc tggctcttca 1021 atggctccgt gctcaatgag accagcttca tcttcactga gttcctggag ccggcagcca 1081 atgagaccgt gcggcacggg tgtctgcgcc tcaaccagcc cacccacgtc aacaacggca 1141 actacacgct gctggctgcc aaccccttcg gccaggcctc cgcctccatc atggctgcct 1201 tcatggacaa ccctttcgag ttcaaccccg aggaccccat ccctgacact aacagcacat 1261 ctggagaccc ggtggagaag aaggacgaaa caccttttgg ggtctcggtg gctgtgggcc 1321 tggccgtctt tgcctgcctc ttcctttcta cgctgctcct tgtgctcaac aaatgtggac 1381 ggagaaacaa gtttgggatc aaccgcccgg ctgtgctggc tccagaggat gggctggcca 1441 tgtccctgca tttcatgaca ttgggtggca gctccctgtc ccccaccgag ggcaaaggct 1501 ctgggctcca aggccacatc atcgagaacc cacaatactt cagtgatgcc tgtgttcacc 1561 acatcaagcg ccgggacatc gtgctcaagt gggagctggg ggagggcgcc tttgggaagg 1621 tcttccttgc tgagtgccac aacctcctgc ctgagcagga caagatgctg gtggctgtca 1681 aggcactgaa ggaggcgtcc gagagtgctc ggcaggactt ccagcgtgag gctgagctgc 1741 tcaccatgct gcagcaccag cacatcgtgc gcttcttcgg cgtctgcacc gagggccgcc 1801 ccctgctcat ggtctttgag tatatgcggc acggggacct caaccgcttc ctccgatccc 1861 atggacctga tgccaagctg ctggctggtg gggaggatgt ggctccaggc cccctgggtc 1921 tggggcagct gctggccgtg gctagccagg tcgctgcggg gatggtgtac ctggcgggtc 1981 tgcattttgt gcaccgggac ctggccacac gcaactgtct agtgggCCAG GGACTGGTGG 2041 TCAAGATTGG TGATTTTGGC ATGAGCAGGG ATATCTACAG CACCGACTAT TACCGTGTGG 2101 GAGGCCGCAC CATGCTGCCC ATTCGCTGGA TGCCGCCCGA GAGCATCCTG TACCGTAAGT 2161 TCACCACCGA GAGCGACGTG TGGAGCTTCG GCGTGGTGCT CTGGGAGATC TTCACCTACG 2221 GCAAGCAGCC CTGGTACCAG CTCTCCAACA CGGAGGCAAT CGACTGCATC ACGCAGGGAC 2281 GTGAGTTGGA GCGGCCACGT GCCTGCCCAC CAGAGGTCTA CGCCATCATG CGGGGCTGCT 2341 GGCAGCGGGA GCCCCAGCAA CGCCACAGCA TCAAGGATGT GCACGCCCGG CTGCAAGCCC 2401 TGGCCCAGGC ACCTCCTGTC TACCTGGATG TCCTGGGCCC AACTTTCTTG TACAAAGTGG 2461 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2521 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2581 ACGAATCGAA CTGAGCTTCC AGCTAACGCG TTAAGTCgac aatcaacctc tggattacaa 2641 aatttgtgaa agatt