Construct: ORF TRCN0000469134
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012009.1_s317c1
- Derived from:
- ccsbBroadEn_05732
- DNA Barcode:
- GATCCATCCTAGTGGCTCACGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSAG2 (728461)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469134
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389903 | CSAG3 | CSAG family member 3 | NM_001129828.3 | 100% | 100% | |
| 2 | human | 102723547 | CSAG2 | CSAG family member 2 | XM_017030023.1 | 99.6% | 99% | 187A>G |
| 3 | human | 389903 | CSAG3 | CSAG family member 3 | NM_001129826.3 | 86.6% | 86.6% | 264_314del |
| 4 | human | 389903 | CSAG3 | CSAG family member 3 | XM_024452379.1 | 86.6% | 86.6% | 264_314del |
| 5 | human | 102723547 | CSAG2 | CSAG family member 2 | XM_006724857.3 | 86.3% | 85.8% | 187A>G;264_314del |
| 6 | human | 158511 | CSAG1 | chondrosarcoma associated g... | NM_001102576.3 | 66% | 59% | (many diffs) |
| 7 | human | 158511 | CSAG1 | chondrosarcoma associated g... | NM_153478.3 | 66% | 59% | (many diffs) |
| 8 | human | 102723547 | CSAG2 | CSAG family member 2 | XR_938529.2 | 38% | (many diffs) | |
| 9 | human | 100130935 | CSAG4 | CSAG family member 4 (pseud... | NR_073432.1 | 36.8% | (many diffs) | |
| 10 | human | 389903 | CSAG3 | CSAG family member 3 | NR_130638.1 | 36.5% | (many diffs) | |
| 11 | human | 102723547 | CSAG2 | CSAG family member 2 | XR_938528.2 | 24% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 399
- ORF length:
- 330
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GTGGATGGGC CTCATCCAAT TAGTTGAAGG TGTTAAGAGA AAAGACCAAG 121 GTTTCCTGGA AAAGGAATTC TACCACAAGA CTAACATAAA AATGCGCTGT GAGTTTCTAG 181 CCTGCTGGCC TGCCTTCACT GTCCTGGGGG AGGCTTGGAG AGACCAGGTG GACTGGAGTA 241 GACTGTTGAG AGACGCTGGT CTGGTGAAGA TGTCCAGGAA ACCACGAGCC TCCAGCCCAT 301 TGTCCAACAA CCACCCACCA ACACCAAAGA GGTTCCCAAG ACAACCCGGA AGGGAAAAGG 361 GACCCATCAA GGAAGTTCCA GGAACAAAAG GCTCTCCCTT GCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGAGATCCA TCCTAGTGGC TCACGGACAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt