Construct: ORF TRCN0000469220
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016532.1_s317c1
- Derived from:
- ccsbBroadEn_06764
- DNA Barcode:
- CAAGGACAGTGCATGGTCCCCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRB3 (5544)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469220
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5544 | PRB3 | proline rich protein BstNI ... | NM_006249.5 | 99.4% | 99% | (many diffs) |
| 2 | human | 5542 | PRB1 | proline rich protein BstNI ... | NM_005039.3 | 77.9% | 68.6% | (many diffs) |
| 3 | human | 5545 | PRB4 | proline rich protein BstNI ... | NM_002723.6 | 73.3% | 68% | (many diffs) |
| 4 | human | 5542 | PRB1 | proline rich protein BstNI ... | NR_160307.1 | 68% | (many diffs) | |
| 5 | human | 653247 | PRB2 | proline rich protein BstNI ... | NM_006248.4 | 64.4% | 59.1% | (many diffs) |
| 6 | human | 5545 | PRB4 | proline rich protein BstNI ... | NM_001261399.2 | 53.4% | 50.6% | (many diffs) |
| 7 | human | 5542 | PRB1 | proline rich protein BstNI ... | NM_001367912.1 | 44.5% | 41.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 996
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctactgatt ctgctgtcgg tggccctgct ggccctgagc tcagctcaga 121 gcttaaatga agatgtcagc caggaagaat ctccctccgt aatatcagga aagccagaag 181 gacgacgccc acaaggagga aaccagcccc aacgtacccc acctcctcca ggaaagccag 241 aaggacgacc cccacaagga ggcaaccagt cccaaggtcc cccacctcgt ccaggaaagc 301 cagaaggacc acccccacaa ggaggaaacc agtcccaagg tcccccacct cgtccgggaa 361 agccagaagg acnaccccca caaggaggaa accagtccca aggtccccca cctcgtccgg 421 gaaagccaga aggaccaccc ccacaaggag gaaaccagtc ccaaggtccc ccgcctcgtc 481 cgggaaagcc agaaggacca cccccacaag gaggaaacca gtcccaaggt cccccgcctc 541 gtccgggaaa gccagaagga ccacccccac aaggaggaaa ccagtcccaa ggtcccccac 601 ctcgtccggg aaagccagaa ggaccacccc cacaaggagg aaaccagtcc caaggtcccc 661 cgcctcgtcc gggaaagcca gaaggaccac ccccacaagg aggaaaccag tcccaaggtc 721 ccccacctcg tccaggaaag ccagaaggat caccttcaca aggaggcaac aaacctcaag 781 gtcccccacc tcatccagga aagccacaag gaccaccccc acaagaaggt aacaaacctc 841 aacgtccccc tccTCCAGGA AGGCCACAAG GACCACCCCC ACCAGGAGGC AATCCCCAGC 901 AGCCTCTGCC ACCTCCCGCT GGAAAGCCCC AGGGACCACC TCCACCTCCT CAAGGGGGCA 961 GACCACACAG ACCTCCCCAG GGACAGCCTC CCCAGTTGCC AACTTTCTTG TACAAAGTGG 1021 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1081 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1141 ACAAGGACAG TGCATGGTCC CCGACACGCG TTAAGTCgac aatcaacctc tggattacaa 1201 aatttgtgaa agatt