Construct: ORF TRCN0000469322
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007657.1_s317c1
- Derived from:
- ccsbBroadEn_11510
- DNA Barcode:
- TGGATATCTCGTAGCTAACCACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA4F (10505)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469322
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10505 | SEMA4F | ssemaphorin 4F | NM_001271661.2 | 100% | 100% | |
| 2 | human | 10505 | SEMA4F | ssemaphorin 4F | XM_017003173.1 | 84.1% | 69.6% | (many diffs) |
| 3 | human | 10505 | SEMA4F | ssemaphorin 4F | NM_001271662.1 | 83.4% | 83.4% | 357_722del |
| 4 | human | 10505 | SEMA4F | ssemaphorin 4F | XM_017003174.1 | 83.4% | 83.4% | 0_1ins306 |
| 5 | human | 10505 | SEMA4F | ssemaphorin 4F | XM_017003172.1 | 81.1% | 66.6% | (many diffs) |
| 6 | human | 10505 | SEMA4F | ssemaphorin 4F | NM_004263.5 | 79.8% | 79.8% | 357_821del |
| 7 | human | 10505 | SEMA4F | ssemaphorin 4F | XM_011532475.3 | 60.1% | 60.2% | (many diffs) |
| 8 | human | 10505 | SEMA4F | ssemaphorin 4F | XR_001738588.2 | 58.5% | (many diffs) | |
| 9 | human | 10505 | SEMA4F | ssemaphorin 4F | XM_011532476.3 | 55.2% | 54% | (many diffs) |
| 10 | human | 10505 | SEMA4F | ssemaphorin 4F | XM_011532477.3 | 55% | 54% | (many diffs) |
| 11 | human | 10505 | SEMA4F | ssemaphorin 4F | XR_001738590.1 | 40.9% | (many diffs) | |
| 12 | human | 10505 | SEMA4F | ssemaphorin 4F | XR_939650.3 | 40% | (many diffs) | |
| 13 | human | 10505 | SEMA4F | ssemaphorin 4F | NR_073398.2 | 31.6% | 1_130del;487_705del;2195_5829del | |
| 14 | human | 10505 | SEMA4F | ssemaphorin 4F | NR_073399.2 | 31.3% | 1_130del;487_758del;2248_5882del | |
| 15 | mouse | 20355 | Sema4f | sema domain, immunoglobulin... | NM_001308374.1 | 71.7% | 75.9% | (many diffs) |
| 16 | mouse | 20355 | Sema4f | sema domain, immunoglobulin... | XM_011241264.1 | 71.7% | 75.9% | (many diffs) |
| 17 | mouse | 20355 | Sema4f | sema domain, immunoglobulin... | XM_011241265.2 | 71.7% | 75.9% | (many diffs) |
| 18 | mouse | 20355 | Sema4f | sema domain, immunoglobulin... | NM_011350.4 | 68.8% | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1911
- ORF length:
- 1845
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggcctctgct gcgcggcccc gcccgggtcc cgggcagcct acagcctcgc 121 ccttcccgct actgctgctg gcggtgctga gcggcccggt atccggccgc gtcccccgct 181 cggtgcccag aacctcgctt ccaatctctg aggctgactc ctgtctcacc cggttcgcag 241 tccctcacac atacaattac tctgttctcc ttgtggatcc tgcctcccac acactttatg 301 ttggcgcccg ggacaccatc ttcgctttat ccctgccctt ctcaggggag agaccccgca 361 ggattgactg gatggttcct gaggctcaca gacagaactg taggaagaaa ggcaagaaag 421 agggggacct cgggggccgg aagaccctcc agcagagatg gacgacgttt ttgaaagctg 481 acctgctctg tccagggcct gagcatggcc gggcctccag tgtcctgcag gatgttgctg 541 tgcttcgacc tgagcttggg gcagggactc ccatctttta tggcatcttt tcttcccagt 601 gggagggggc tactatctct gctgtctgtg ccttccgacc acaagacatt cggacagtgc 661 tgaatggtcc cttcagagaa ctaaaacatg actgcaacag aggactgcct gtcgtggaca 721 atgatgtgcc ccagcccaga cctggagagt gcatcaccaa caacatgaag ctccggcact 781 ttggctcatc tctctccctg cctgaccgcg tactcacctt catccgggac cacccactca 841 tggacaggcc agtgtttcca gctgatggcc accccctgct ggtcactaca gatacagcct 901 atctcagagt cgtggcccac agggtgacca gcctctcagg gaaagagtat gatgtgctct 961 acctggggac agaggatgga cacctccacc gagcagtgcg gatcggagct cagctcagcg 1021 ttcttgaaga tctggcctta ttcccagagc cacagccagt tgagaacatg aaattgtacc 1081 acagctggct cctggttggc tcccgtactg aggtgacaca agtgaataca accaactgtg 1141 gccgtctcca gagctgctca gagtgcatcc tggcccagga cccagtctgt gcctggagct 1201 tccggctgga tgagtgtgtg gcccatgccg gggagcaccg agggttggtc caagacatag 1261 agtcagcaga tgtctcctct ttgtgtccta aagagcctgg agaacgtcca gtagtgtttg 1321 aagttcccgt ggctacagct gcgcatgtgg tcttgccatg ttctccaagc tcagcatggg 1381 catcctgtgt gtggcaccag cccagtggag tgactgcact caccccccgg cgggatggac 1441 tGGAGGTGGT GGTGACCCCA GGGGCCATGG GCGCTTATGC CTGTGAATGT CAGGAGGGTG 1501 GGGCAGCCCA TGTGGTAGCA GCTTACAGCT TGGTATGGGG CAGCCAGCGA GATGCTCCGA 1561 GCCGGGCCCA CACAGTGGGG GCGGGACTGG CTGGCTTCTT CTTGGGGATT CTCGCAGCAT 1621 CCCTGACTCT CATTCTGATT GGTCGGCGTC AGCAGCGACG GCGACAGAGG GAACTTCTGG 1681 CTAGAGACAA GGTGGGCCTG GACCTGGGGG CTCCACCTTC TGGGACCACA AGCTACAGCC 1741 AAGACCCTCC CTCCCCCTCT CCTGAAGATG AGCGGTTGCC GCTGGCCCTG GCCAAGAGGG 1801 GCAGTGGCTT TGGTGGATTC TCACCACCCT TCCTGCTTGA TCCTTGCCCA AGCCCAGCCC 1861 ACATTCGGCT AACTGGGGCT CCTCTAGCCA CATGTGATGA AACATCCATC TACCCAACTT 1921 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1981 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2041 CTTGTGGAAA GGACGATGGA TATCTCGTAG CTAACCACTC ACGCGTTAAG TCgacaatca 2101 acctctggat tacaaaattt gtgaaagatt