Transcript: Human NR_073398.2

Homo sapiens ssemaphorin 4F (SEMA4F), transcript variant 4, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
SEMA4F (10505)
Length:
5829
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_073398.2
NBCI Gene record:
SEMA4F (10505)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_073398.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000112273 CAGACAGAACTGTAGGAAGAA pLKO.1 454 3UTR 100% 4.950 3.465 N Sema4f n/a
2 TRCN0000112272 CTGTAGGAAGAAAGGCAAGAA pLKO.1 463 3UTR 100% 4.950 3.465 N Sema4f n/a
3 TRCN0000061128 CAAGACATAGAGTCAGCAGAT pLKO.1 1535 3UTR 100% 4.050 2.835 N SEMA4F n/a
4 TRCN0000291256 CAAGACATAGAGTCAGCAGAT pLKO_005 1535 3UTR 100% 4.050 2.835 N SEMA4F n/a
5 TRCN0000061132 GCCCAGAACCTCGCTTCCAAT pLKO.1 250 3UTR 100% 1.650 1.155 N SEMA4F n/a
6 TRCN0000291258 GCCCAGAACCTCGCTTCCAAT pLKO_005 250 3UTR 100% 1.650 1.155 N SEMA4F n/a
7 TRCN0000061131 CTGAGGTGACACAAGTGAATA pLKO.1 1392 3UTR 100% 13.200 7.920 N SEMA4F n/a
8 TRCN0000291257 CTGAGGTGACACAAGTGAATA pLKO_005 1392 3UTR 100% 13.200 7.920 N SEMA4F n/a
9 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 4142 3UTR 100% 4.950 2.475 Y ERAP2 n/a
10 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 4143 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_073398.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11510 pDONR223 100% 31.6% None 1_130del;487_705del;2195_5829del n/a
2 ccsbBroad304_11510 pLX_304 0% 31.6% V5 1_130del;487_705del;2195_5829del n/a
3 TRCN0000469322 TGGATATCTCGTAGCTAACCACTC pLX_317 25.7% 31.6% V5 1_130del;487_705del;2195_5829del n/a
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