Construct: ORF TRCN0000469328
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014278.1_s317c1
- Derived from:
- ccsbBroadEn_01264
- DNA Barcode:
- ACGCTCCCCGTTCAGGGCGGTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP2R2B (5521)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469328
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5521 | PPP2R2B | protein phosphatase 2 regul... | NM_001271948.1 | 96.7% | 95% | (many diffs) |
| 2 | human | 5521 | PPP2R2B | protein phosphatase 2 regul... | NM_181678.2 | 96.7% | 95% | (many diffs) |
| 3 | human | 5521 | PPP2R2B | protein phosphatase 2 regul... | NM_181676.2 | 95.9% | 93.8% | (many diffs) |
| 4 | human | 5521 | PPP2R2B | protein phosphatase 2 regul... | NM_181677.2 | 95.1% | 94.5% | (many diffs) |
| 5 | human | 5521 | PPP2R2B | protein phosphatase 2 regul... | NM_001271899.1 | 95.1% | 92.5% | (many diffs) |
| 6 | human | 5521 | PPP2R2B | protein phosphatase 2 regul... | NM_001271900.2 | 88.4% | 88.4% | 1_174del |
| 7 | human | 5521 | PPP2R2B | protein phosphatase 2 regul... | NM_181674.2 | 87% | 87% | 1_198del |
| 8 | human | 5521 | PPP2R2B | protein phosphatase 2 regul... | NM_181675.3 | 80.6% | 80.6% | 1_318del |
| 9 | human | 5521 | PPP2R2B | protein phosphatase 2 regul... | NR_073527.1 | 62.3% | 1_351del;1681_2130del | |
| 10 | human | 5521 | PPP2R2B | protein phosphatase 2 regul... | NR_073526.2 | 11.8% | 1_686del;2016_11223del | |
| 11 | mouse | 72930 | Ppp2r2b | protein phosphatase 2, regu... | NM_028392.3 | 90.8% | 93.8% | (many diffs) |
| 12 | mouse | 72930 | Ppp2r2b | protein phosphatase 2, regu... | NR_073583.1 | 49.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1395
- ORF length:
- 1329
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaggacatt gatacccgca aaatcaacaa cagtttcctg cgcgaccaca 121 gctatgcgac cgaagctgac attatctcta cggtagaatt caaccacacg ggagaattac 181 tagcgacagg ggacaagggg ggtcgggttg taatatttca acgagagcag gagagtaaaa 241 atcaggttca tcgtaggggt gaatacaatg tttacagcac attccagagc catgaacccg 301 agttcgatta cctgaagagt ttagaaatag aagaaaaaat caataaaata agatggctcc 361 cccagcagaa tgcagcttac tttcttctgt ctactaatga taaaactgtg aagctgtgga 421 aagtcagcga gcgtgataag aggccagaag gctacaatct gaaagatgag gagggccggc 481 tccgggatcc tgccaccatc acaaccctgc gggtgcctgt cctgagaccc atggacctga 541 tggtggaggc caccccacga agagtatttg ccaacgcaca cacatatcac atcaactcca 601 tatctgtcaa cagcgactat gaaacctaca tgtccgctga tgacctgagg attaacctat 661 ggaactttga aataaccaat caaagtttta atattgtgga cattaagcca gccaacatgg 721 aggagctcac ggaggtgatc acagcagccg agttccaccc ccatcattgc aacaccttcg 781 tgtacagcag cagcaaaggg acaatccggc tgtgtgacat gcgggcatct gccctgtgtg 841 acaggcacac caaatttttt gaagagccgg aagatccaag caacagatca tttttctctg 901 aaattatctc ttcgatttcg gatgtgaagt tCAGCCACAG TGGGAGGTAT ATCATGACCA 961 GGGACTACTT GACCGTCAAA GTCTGGGATC TCAACATGGA AAACCGCCCC ATCGAGACTT 1021 ACCAGGTTCA TGACTACCTC CGCAGCAAGC TGTGTTCCCT CTATGAAAAT GACTGCATTT 1081 TTGATAAATT TGAGTGTGTG TGGAATGGGT CAGACAGTGT CATCATGACA GGCTCCTACA 1141 ACAACTTCTT CAGGATGTTC GACAGAAACA CCAAGCGTGA TGTGACCCTT GAGGCTTCGA 1201 GGGAAAACAG CAAGCCCCGG GCTATCCTCA AACCCCGAAA AGTGTGTGTG GGGGGCAAGC 1261 GGAGAAAAGA CGAGATCAGT GTCGACAGTC TGGACTTTAG CAAAAAGATC TTGCATACAG 1321 CTTGGCATCC TTCAGAAAAT ATTATAGCAG TGGCGGCTAC AAATAACCTA TATATATTCC 1381 AGGACAAGGT TAACTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1441 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1501 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACGCTCCCCG TTCAGGGCGG 1561 TTCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt