Construct: ORF TRCN0000469339
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015272.1_s317c1
- Derived from:
- ccsbBroadEn_07822
- DNA Barcode:
- ACCTGAGCTCTACTCTGGTAGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKR7A3 (22977)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469339
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22977 | AKR7A3 | aldo-keto reductase family ... | NM_012067.3 | 99.6% | 99.3% | 501G>C;643A>G;967A>G |
| 2 | human | 246181 | AKR7L | aldo-keto reductase family ... | NM_001348421.1 | 95.8% | 93% | (many diffs) |
| 3 | human | 8574 | AKR7A2 | aldo-keto reductase family ... | NM_003689.3 | 83.4% | 81.3% | (many diffs) |
| 4 | human | 22977 | AKR7A3 | aldo-keto reductase family ... | XM_011541046.3 | 81.4% | 74% | (many diffs) |
| 5 | human | 8574 | AKR7A2 | aldo-keto reductase family ... | NM_001320979.1 | 74.1% | 71.8% | (many diffs) |
| 6 | human | 246182 | AKR7A2P1 | aldo-keto reductase family ... | NR_002796.2 | 62.8% | (many diffs) | |
| 7 | human | 22977 | AKR7A3 | aldo-keto reductase family ... | XM_017000714.2 | 62.8% | 55.2% | (many diffs) |
| 8 | human | 8574 | AKR7A2 | aldo-keto reductase family ... | XR_946785.2 | 59.4% | (many diffs) | |
| 9 | human | 22977 | AKR7A3 | aldo-keto reductase family ... | XR_001737055.1 | 49% | (many diffs) | |
| 10 | human | 246181 | AKR7L | aldo-keto reductase family ... | NR_040288.2 | 39.9% | (many diffs) | |
| 11 | human | 246181 | AKR7L | aldo-keto reductase family ... | NR_040289.2 | 32.1% | (many diffs) | |
| 12 | mouse | 110198 | Akr7a5 | aldo-keto reductase family ... | NM_025337.3 | 77.9% | 77.1% | (many diffs) |
| 13 | mouse | 110198 | Akr7a5 | aldo-keto reductase family ... | XR_390721.3 | 51.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1059
- ORF length:
- 993
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ccggcagctg tcgcgggccc ggccagccac ggtgctgggc gccatggaga 121 tggggcgccg catggacgcg cccaccagcg ccgcagtcac gcgcgccttc ctggagcgcg 181 gccacaccga gatagacacg gccttcgtgt acagcgaggg ccagtccgag accatccttg 241 gcggcctggg gctccggctg ggcggcagcg actgcagagt gaaaattgat accaaggcca 301 ttccactgtt tgggaactcc ctgaagcctg acagtctccg gttccagctg gagacgtcac 361 tgaagcggct gcagtgtccc cgagtggacc tcttctacct gcatatgcca gaccacagca 421 ccccggtgga agagacactg cgtgcctgcc accagctgca ccaggagggc aagttcgtgg 481 agcttggcct ctccaactat gcagcctggg aagtggccga gatctgtacc ctctgcaaga 541 gcaacggctg gatcctgccc actgtctacc agggcatgta caatgccatc acccggcagg 601 tggaaacgga gctcttcccc tgcctcaggc actttggact gaggTTCTAT GCCTTCAACC 661 CTCTGGCTGG GGGCCTGCTG ACCGGCAAGT ACAAGTATGA GGACAAGGAT GGGAAACAGC 721 CCGTGGGCCG CTTCTTTGGG AATACCTGGG CAGAGATGTA CAGGAATCGC TACTGGAAGG 781 AGCACCACTT TGAGGGCATT GCCCTGGTGG AGAAGGCCCT GCAGGCCGCG TATGGCGCCA 841 GCGCCCCCAG CATGACCTCG GCCACCCTCC GGTGGATGTA CCACCACTCA CAGCTGCAGG 901 GTGCCCACGG GGACGCGGTC ATCCTGGGCA TGTCCAGCCT GGAGCAGCTG GAGCAGAACT 961 TGGCAGCGGC AGAGGAAGGG CCCCTGGAGC CGGCTGTCGT GGACGCCTTT AATCAAGCCT 1021 GGCATTTGGT TGCTCACGAA TGTCCCAACT ACTTCCGCTA CCCAACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGAACCTGA GCTCTACTCT GGTAGCAAAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt