Construct: ORF TRCN0000469368
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008942.1_s317c1
- Derived from:
- ccsbBroadEn_00361
- DNA Barcode:
- TCGCTTTTGTCGAGAGAGTTTCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATF2 (1386)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469368
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1386 | ATF2 | activating transcription fa... | NM_001256090.2 | 100% | 100% | |
| 2 | human | 1386 | ATF2 | activating transcription fa... | NM_001880.4 | 100% | 100% | |
| 3 | human | 1386 | ATF2 | activating transcription fa... | NM_001256091.1 | 96.4% | 96.4% | 0_1ins54 |
| 4 | human | 1386 | ATF2 | activating transcription fa... | NM_001256092.2 | 88.5% | 86.4% | 0_1ins77;24_25ins97 |
| 5 | human | 1386 | ATF2 | activating transcription fa... | NM_001256093.2 | 73.9% | 71.7% | (many diffs) |
| 6 | human | 1386 | ATF2 | activating transcription fa... | NM_001256094.1 | 41.3% | 41.3% | 627_627delGins889 |
| 7 | human | 1386 | ATF2 | activating transcription fa... | NR_045771.2 | 35.7% | 1_296del;1479_1552del;1886_4238del | |
| 8 | human | 1386 | ATF2 | activating transcription fa... | NR_045768.2 | 34.7% | (many diffs) | |
| 9 | human | 1386 | ATF2 | activating transcription fa... | NR_045769.2 | 34.4% | (many diffs) | |
| 10 | human | 1386 | ATF2 | activating transcription fa... | NR_045774.2 | 33.6% | 1_296del;922_923ins115;1697_4049del | |
| 11 | human | 1386 | ATF2 | activating transcription fa... | NR_045773.2 | 33.4% | (many diffs) | |
| 12 | human | 1386 | ATF2 | activating transcription fa... | NR_045772.2 | 33.1% | (many diffs) | |
| 13 | human | 1386 | ATF2 | activating transcription fa... | NR_045770.2 | 32.1% | (many diffs) | |
| 14 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001025093.2 | 91% | 95.6% | (many diffs) |
| 15 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284369.1 | 83.1% | 86.1% | (many diffs) |
| 16 | mouse | 11909 | Atf2 | activating transcription fa... | NM_009715.3 | 83.1% | 86.1% | (many diffs) |
| 17 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284376.1 | 81.7% | 84.3% | (many diffs) |
| 18 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284370.1 | 73% | 73.2% | (many diffs) |
| 19 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284371.1 | 73% | 73.2% | (many diffs) |
| 20 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284372.1 | 72% | 76.2% | (many diffs) |
| 21 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284373.1 | 72% | 76.2% | (many diffs) |
| 22 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284374.1 | 46.3% | 49.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1584
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaattcaag ttacatgtga attctgccag gcaatacaag gacctgtgga 121 atatgagtga tgacaaaccc tttctatgta ctgcgcctgg atgtggccag cgttttacca 181 acgaggatca tttggctgtc cataaacata aacatgagat gacactgaaa tttggtccag 241 cacgtaatga cagtgtcatt gtggctgatc agaccccaac accaacaaga ttcttgaaaa 301 actgtgaaga agtgggtttg tttaatgagt tggcgagtcc atttgagaat gaattcaaga 361 aagcttcaga agatgacatt aaaaaaatgc ctctagattt atcccctctt gcaacaccta 421 tcataagaag caaaattgag gagccttctg ttgtagaaac aactcaccag gatagtcctt 481 tacctcaccc agagtctact accagtgatg agaaggaagt accattggca caaactgcac 541 agcccacatc agctattgtt cgtccagcat cattacaggt tcccaatgtg ctgcttacaa 601 gttctgactc aagtgtaatt attcagcagg cagtaccttc accaacctca agtactgtaa 661 tcacccaggc accatcctct aacaggccaa ttgtccctgt accaggccca tttcctcttc 721 tgttacatct tcctaatgga caaaccatgc ctgttgctat tcctgcatca attacaagtt 781 ctaatgtgca tgttccagct gcagtcccac tcgttcgacc agtcaccatg gtgcctagtg 841 ttccaggaat cccaggtcct tcctctcccc aaccagtaca gtcagaagca aaaatgagat 901 taaaagctgc tttgacccag caacatcctc cagttaccaa tggtgatact gtcaaaggtc 961 atggtagcgg attggttagg actcagtcag aggaatctcg accgcagtca ttacaacagc 1021 cagccacatc cactacagaa actccggctt ctccagctca cacaactcca cagacccaaa 1081 gtacaagtgg tcgtcggaga agagcagcta acgaagatcc TGATGAAAAA AGGAGAAAGT 1141 TTTTAGAGCG AAATAGAGCA GCAGCTTCAA GATGCCGACA AAAAAGGAAA GTCTGGGTTC 1201 AGTCTTTAGA GAAGAAAGCT GAAGACTTGA GTTCATTAAA TGGTCAGCTG CAGAGTGAAG 1261 TCACCCTGCT GAGAAATGAA GTGGCACAGC TGAAACAGCT TCTTCTGGCT CATAAAGATT 1321 GCCCTGTAAC CGCCATGCAG AAGAAATCTG GCTATCATAC TGCTGATAAA GATGATAGTT 1381 CAGAAGACAT TTCAGTGCCG AGTAGTCCAC ATACAGAAGC TATACAGCAT AGTTCGGTCA 1441 GCACATCCAA TGGAGTCAGT TCAACCTCCA AGGCAGAAGC TGTAGCCACT TCAGTCCTCA 1501 CCCAGATGGC GGACCAGAGT ACAGAGCCTG CTCTTTCACA GATCGTTATG GCTCCTTCCT 1561 CCCAGTCACA GCCCTCAGGA AGTTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1621 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1681 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CGCTTTTGTC 1741 GAGAGAGTTT CCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1801 att