Transcript: Human NM_001256092.2

Homo sapiens activating transcription factor 2 (ATF2), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
ATF2 (1386)
Length:
4067
CDS:
374..1717

Additional Resources:

NCBI RefSeq record:
NM_001256092.2
NBCI Gene record:
ATF2 (1386)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001256092.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000013713 GCGAAATCTGTGGTTGTAAAT pLKO.1 1799 3UTR 100% 13.200 18.480 N ATF2 n/a
2 TRCN0000219047 TGCGAAATCTGTGGTTGTAAA pLKO_005 1798 3UTR 100% 13.200 18.480 N ATF2 n/a
3 TRCN0000374121 TGCGAAATCTGTGGTTGTAAA pLKO_005 1798 3UTR 100% 13.200 18.480 N Atf2 n/a
4 TRCN0000229648 ATGAGTTGGCGAGTCCATTTG pLKO_005 456 CDS 100% 10.800 15.120 N ATF2 n/a
5 TRCN0000013716 GCAGCTAACGAAGATCCTGAT pLKO.1 1235 CDS 100% 4.050 3.240 N ATF2 n/a
6 TRCN0000229649 CTCTTGCAACACCTATCATAA pLKO_005 537 CDS 100% 13.200 9.240 N ATF2 n/a
7 TRCN0000013715 CCATCCTCTAACAGGCCAATT pLKO.1 803 CDS 100% 10.800 7.560 N ATF2 n/a
8 TRCN0000218254 TGCTCTTTCACAGATCGTTAT pLKO_005 1660 CDS 100% 10.800 7.560 N ATF2 n/a
9 TRCN0000082080 CCTCCAGTTACCAATGGTGAT pLKO.1 1058 CDS 100% 4.050 2.835 N Atf2 n/a
10 TRCN0000013714 GCATCATTACAGGTTCCCAAT pLKO.1 698 CDS 100% 4.050 2.835 N ATF2 n/a
11 TRCN0000013717 GCTCATAAAGATTGCCCTGTA pLKO.1 1439 CDS 100% 4.050 2.835 N ATF2 n/a
12 TRCN0000218996 GCTATCATACTGCTGATAAAG pLKO_005 1482 CDS 100% 1.320 0.924 N ATF2 n/a
13 TRCN0000082082 CTGGCTATCATACTGCTGATA pLKO.1 1479 CDS 100% 0.495 0.347 N Atf2 n/a
14 TRCN0000365934 TTGCTATTCCTGCATCAATTA pLKO_005 885 CDS 100% 13.200 7.920 N Atf2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001256092.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00361 pDONR223 100% 88.5% 86.4% None 0_1ins77;24_25ins97 n/a
2 ccsbBroad304_00361 pLX_304 44.6% 88.5% 86.4% V5 0_1ins77;24_25ins97 n/a
3 TRCN0000469368 TCGCTTTTGTCGAGAGAGTTTCCG pLX_317 30.8% 88.5% 86.4% V5 0_1ins77;24_25ins97 n/a
4 ccsbBroadEn_10749 pDONR223 100% 29.8% 28.2% None 0_1ins77;24_25ins97;453_1341delinsG n/a
5 ccsbBroad304_10749 pLX_304 0% 29.8% 28.2% V5 0_1ins77;24_25ins97;453_1341delinsG n/a
6 TRCN0000468300 CCCGGTGGTGACCGCTCCGGTCGA pLX_317 57.9% 29.8% 28.2% V5 0_1ins77;24_25ins97;453_1341delinsG n/a
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