Construct: ORF TRCN0000469460
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016601.2_s317c1
- Derived from:
- ccsbBroadEn_14893
- DNA Barcode:
- CTTGGGCACCAGGCGCTTACTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK24 (8428)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469460
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8428 | STK24 | serine/threonine kinase 24 | NM_001032296.3 | 100% | 100% | |
| 2 | human | 8428 | STK24 | serine/threonine kinase 24 | NM_003576.4 | 96% | 95% | (many diffs) |
| 3 | human | 8428 | STK24 | serine/threonine kinase 24 | NM_001286649.1 | 95.5% | 95.5% | 273_274ins57 |
| 4 | human | 8428 | STK24 | serine/threonine kinase 24 | XM_017020794.2 | 82.6% | 81.6% | (many diffs) |
| 5 | human | 8428 | STK24 | serine/threonine kinase 24 | XM_005254079.5 | 77.1% | 77.2% | 0_1ins294;774G>A |
| 6 | human | 8428 | STK24 | serine/threonine kinase 24 | XM_024449426.1 | 77.1% | 77.2% | 0_1ins294;774G>A |
| 7 | mouse | 223255 | Stk24 | serine/threonine kinase 24 | NM_145465.2 | 88.1% | 97.6% | (many diffs) |
| 8 | mouse | 223255 | Stk24 | serine/threonine kinase 24 | XM_011245043.2 | 86.1% | 94.9% | (many diffs) |
| 9 | mouse | 223255 | Stk24 | serine/threonine kinase 24 | XM_017315988.1 | 85% | 92.1% | (many diffs) |
| 10 | mouse | 223255 | Stk24 | serine/threonine kinase 24 | XM_006518918.1 | 67.6% | 74.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1359
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcactccccg gtgcagtcgg gcctgcccgg catgcagaac ctaaaggcag 121 acccagaaga gctttttaca aaactagaga aaattgggaa gggctccttt ggagaggtgt 181 tcaaaggcat tgacaatcgg actcagaaag tggttgccat aaagatcatt gatctggaag 241 aagctgaaga tgagatagag gacattcaac aagaaatcac agtgctgagt cagtgtgaca 301 gtccatatgt aaccaaatat tatggatcct atctgaagga tacaaaatta tggataataa 361 tggaatatct tggtggaggc tccgcactag atctattaga acctggccca ttagatgaaa 421 cccagatcgc tactatatta agagaaatac tgaaaggact cgattatctc cattcggaga 481 agaaaatcca cagagacatt aaagcggcca acgtcctgct gtctgagcat ggcgaggtga 541 agctggcgga ctttggcgtg gctggccagc tgacagacac ccagatcaaa aggaacacct 601 tcgtgggcac cccattctgg atggcacccg aggtcatcaa acagtcggcc tatgactcga 661 aggcagacat ctggtccctg ggcataacag ctattgaact tgcaagaggg gaaccacctc 721 attccgagct gcaccccatg aaagttttat tcctcattcc aaagaacaac ccaccgacgt 781 tggaaggaaa ctacagtaaa cccctcaagg agtttgtgga ggcctgtttg aataaggagc 841 cgagctttag acccactgct aaggagttat tgaagcacaa gtttatacta cgcaatgcaa 901 agaaaacttc ctacttgacc gagctcatcg acaggtacaa gagatggaag gccgagcaga 961 gccatgacga ctcgagctcc gaggattccg acgcggaaac agatggccaa gcctcggggg 1021 gcagtgattc tgggGACTGG ATCTTCACAA TCCGAGAAAA AGATCCCAAG AATCTCGAGA 1081 ATGGAGCTCT TCAGCCATCG GACTTGGACA GAAATAAGAT GAAAGACATC CCAAAGAGGC 1141 CTTTCTCTCA GTGTTTATCT ACAATTATTT CTCCTCTGTT TGCAGAGTTG AAGGAGAAGA 1201 GCCAGGCGTG CGGAGGGAAC TTGGGGTCCA TTGAAGAGCT GCGAGGGGCC ATCTACCTAG 1261 CGGAGGAGGC GTGCCCTGGC ATCTCCGACA CCATGGTGGC CCAGCTCGTG CAGCGGCTCC 1321 AGAGATACTC TCTAAGTGGT GGAGGAACTT CATCCCACTG CCCAACTTTC TTGTACAAAG 1381 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1441 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1501 ACGACTTGGG CACCAGGCGC TTACTTTCAC GCGTTAAGTC gacaatcaac ctctggatta 1561 caaaatttgt gaaagatt