Construct: ORF TRCN0000469466
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004722.1_s317c1
- Derived from:
- ccsbBroadEn_13576
- DNA Barcode:
- GACCATCATCATCTAGAATTTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBAC2 (337867)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469466
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 337867 | UBAC2 | UBA domain containing 2 | XM_017020553.1 | 90.2% | 90.2% | 1_51del |
| 2 | human | 337867 | UBAC2 | UBA domain containing 2 | XM_006719948.3 | 67.9% | 67.9% | 1_222del |
| 3 | human | 337867 | UBAC2 | UBA domain containing 2 | XM_011521083.2 | 53.7% | 53.7% | 1_405del |
| 4 | human | 337867 | UBAC2 | UBA domain containing 2 | NM_177967.4 | 50.8% | 50.8% | 1_456del |
| 5 | human | 337867 | UBAC2 | UBA domain containing 2 | XM_011521082.2 | 49.5% | 49.5% | 1_480del |
| 6 | human | 337867 | UBAC2 | UBA domain containing 2 | NM_001144072.2 | 45.6% | 45.6% | 1_561del |
| 7 | human | 337867 | UBAC2 | UBA domain containing 2 | NR_026644.2 | 16.3% | 1_1244del;1716_2887del | |
| 8 | mouse | 68889 | Ubac2 | ubiquitin associated domain... | XM_006519493.3 | 82.9% | 83.3% | (many diffs) |
| 9 | mouse | 68889 | Ubac2 | ubiquitin associated domain... | XM_006519492.2 | 40.8% | 41% | (many diffs) |
| 10 | mouse | 68889 | Ubac2 | ubiquitin associated domain... | NM_026861.2 | 38.9% | 39.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cggtctgtgc tacgacagca aaatgttcca ggtgcatcag gtgctctgca 121 tccccagctg gatggcaaaa ttcttttctt ggacacttga acccaTCTTC TCTTCTTCAG 181 AACCCACCAG CGAAGCCAGA ATTGGGATGG GAGCCACGCT GGACATCCAG AGACAGCAGA 241 GAATGGAGCT GCTGGACCGG CAGCTGATGT TCTCTCAGTT TGCACAAGGG AGGCGACAGA 301 GACAGCAGCA GGGAGGAATG ATCAATTGGA ATCGTCTTTT TCCTCCTTTA CGTCAGCGAC 361 AAAACGTAAA CTATCAGGGC GGTCGGCAGT CTGAGCCAGC AGCGCCCCCT CTAGAAGTTT 421 CTGAGGAACA GGTCGCCCGG CTCATGGAGA TGGGATTTTC CAGAGGTGAT GCTTTGGAAG 481 CCCTGAGAGC TTCAAACAAT GACCTCAATG TCGCCACCAA CTTCCTGCTG CAGCACTGCC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GAGACCATCA TCATCTAGAA TTTCAGACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt