Construct: ORF TRCN0000469524
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000096.1_s317c1
- Derived from:
- ccsbBroadEn_07774
- DNA Barcode:
- GCCGTAACAGCGCTCCTATAAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUSP10 (11221)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469524
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11221 | DUSP10 | dual specificity phosphatas... | NM_007207.6 | 99.8% | 100% | 279A>G;726A>G |
| 2 | human | 11221 | DUSP10 | dual specificity phosphatas... | XM_017000147.2 | 57.2% | 56.4% | (many diffs) |
| 3 | human | 11221 | DUSP10 | dual specificity phosphatas... | NR_111940.1 | 30.5% | (many diffs) | |
| 4 | human | 11221 | DUSP10 | dual specificity phosphatas... | NR_111939.1 | 27.3% | (many diffs) | |
| 5 | mouse | 63953 | Dusp10 | dual specificity phosphatas... | NM_022019.5 | 92.2% | 96.4% | (many diffs) |
| 6 | mouse | 63953 | Dusp10 | dual specificity phosphatas... | XM_006497188.2 | 92.2% | 96.4% | (many diffs) |
| 7 | mouse | 63953 | Dusp10 | dual specificity phosphatas... | XM_006497189.1 | 92.2% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1512
- ORF length:
- 1446
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tccgtctcct ttagacgaca gggtagtagt ggcactatct aggcccgtcc 121 gacctcagga tctcaacctt tgtttagact ctagttacct tggctctgcc aacccaggca 181 gtaacagcca ccctcctgtc atcgccacca ccgttgtgtc cctcaaggct gcgaatctga 241 cgtatatgcc ctcatccagc ggctctgccc gctcgctgaa ttgtggatgc agcagtgcca 301 gctgctgcac tgtggcaacc tacgacaagg acaatcaggc ccagacccaa gccattgccg 361 ctggcaccac caccactgcc atcggaacct ctaccacctg ccctgctaac cagatggtca 421 acaataatga gaatacaggc tctctaagtc catcaagtgg ggtgggcagc cctgtgtcag 481 ggacccccaa gcagctagcc agcatcaaaa taatctaccc caatgacttg gcaaagaaga 541 tgaccaaatg cagcaagagt cacctgccga gtcagggccc tgtcatcatt gactgcaggc 601 ccttcatgga gtacaacaag agtcacatcc aaggagctgt ccacattaac tgtgccgata 661 agatcagccg gcggagactg cagcagggca agatcactgt cctagacttg atttcctgta 721 gggaaggcaa ggactctttc aagaggatct tttccaaaga aattatagtt tatgatgaga 781 ataccaatga gccaagccga gtgatgccct cccagccact tcacatagtc ctcgagtccc 841 tgaagagaga aggcaaagaa cctctggtgt tgaaaggtgg acttagtagt tttaagcaga 901 accatgaaaa cctctgtgac aactccctcc agctccaaga gtgccgggag gtggggggcg 961 gcgcatccgc ggcctcgagc ttgctacctc agcccatccc caccacccct gacatcgaga 1021 acgctgagct cacccccatc ttgcccttcc tgttccttgg caatgagcag gatgctcagg 1081 acctggacac catgcagcgg ctgaacatcg gctacgtcat caacgtcacc actcaTCTTC 1141 CCCTCTACCA CTATGAGAAA GGCCTGTTCA ACTACAAGCG GCTGCCAGCC ACTGACAGCA 1201 ACAAGCAGAA CCTGCGGCAG TACTTTGAAG AGGCTTTTGA GTTCATTGAG GAAGCTCACC 1261 AGTGTGGGAA GGGGCTTCTC ATCCACTGCC AGGCTGGGGT GTCCCGCTCC GCCACCATCG 1321 TCATCGCTTA CTTGATGAAG CACACTCGGA TGACCATGAC TGATGCTTAT AAATTTGTCA 1381 AAGGCAAACG ACCAATTATC TCCCCAAACC TTAACTTCAT GGGGCAGTTG CTAGAGTTCG 1441 AGGAAGACCT AAACAACGGT GTGACACCGA GAATCCTTAC ACCAAAGCTG ATGGGCGTGG 1501 AGACGGTTGT GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1561 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1621 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCC GTAACAGCGC TCCTATAAAT 1681 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t