Construct: ORF TRCN0000469603
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010575.1_s317c1
- Derived from:
- ccsbBroadEn_06549
- DNA Barcode:
- CAAACTTGGTTGGCATGCAAAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMAD3 (4088)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469603
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4088 | SMAD3 | SMAD family member 3 | NM_005902.4 | 99.9% | 100% | 309A>G |
| 2 | human | 4088 | SMAD3 | SMAD family member 3 | XM_011521559.3 | 89.5% | 89.6% | 309A>G;396_397ins132 |
| 3 | human | 4088 | SMAD3 | SMAD family member 3 | NM_001145103.1 | 87.8% | 84.3% | (many diffs) |
| 4 | human | 4088 | SMAD3 | SMAD family member 3 | XM_011521560.1 | 84.9% | 83.4% | (many diffs) |
| 5 | human | 4088 | SMAD3 | SMAD family member 3 | NM_001145102.1 | 75.2% | 75.2% | 0_1ins315 |
| 6 | human | 4087 | SMAD2 | SMAD family member 2 | XM_017025746.2 | 73.6% | 89.7% | (many diffs) |
| 7 | human | 4087 | SMAD2 | SMAD family member 2 | NM_001003652.4 | 69.3% | 83.9% | (many diffs) |
| 8 | human | 4087 | SMAD2 | SMAD family member 2 | NM_005901.6 | 69.3% | 83.9% | (many diffs) |
| 9 | human | 4087 | SMAD2 | SMAD family member 2 | XM_005258259.4 | 69.3% | 83.9% | (many diffs) |
| 10 | human | 4087 | SMAD2 | SMAD family member 2 | XM_006722451.4 | 69.3% | 83.9% | (many diffs) |
| 11 | human | 4087 | SMAD2 | SMAD family member 2 | XM_017025745.2 | 69.3% | 83.9% | (many diffs) |
| 12 | human | 4087 | SMAD2 | SMAD family member 2 | XM_024451173.1 | 69.3% | 83.9% | (many diffs) |
| 13 | human | 4088 | SMAD3 | SMAD family member 3 | NM_001145104.1 | 54.1% | 54.1% | 0_1ins585 |
| 14 | human | 4087 | SMAD2 | SMAD family member 2 | XM_017025750.2 | 39.9% | 49.2% | (many diffs) |
| 15 | mouse | 17127 | Smad3 | SMAD family member 3 | NM_016769.4 | 92.9% | 100% | (many diffs) |
| 16 | mouse | 17127 | Smad3 | SMAD family member 3 | XM_006510819.2 | 77.7% | 83.7% | (many diffs) |
| 17 | mouse | 17127 | Smad3 | SMAD family member 3 | XM_006510821.3 | 50.2% | 54.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1341
- ORF length:
- 1275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gtccatcctg cctttcactc ccccgatcgt gaagcgcctg ctgggctgga 121 agaagggcga gcagaacggg caggaggaga aatggtgcga gaaggcggtc aagagcctgg 181 tcaagaaact caagaagacg gggcagctgg acgagctgga gaaggccatc accacgcaga 241 acgtcaacac caagtgcatc accatcccca ggtccctgga tggccggttg caggtgtccc 301 atcggaaggg gctccctcat gtcatctact gccgcctgtg gcgatggcca gacctgcaca 361 gccaccacga gctgcgggcc atggagctgt gtgagttcgc cttcaatatg aagaaggacg 421 aggtctgcgt gaatccctac cactaccaga gagtagagac accagttcta cctcctgtgt 481 tggtgccacg ccacacagag atcccggccg agttcccccc actggacgac tacagccatt 541 ccatccccga aaacactaac ttccccgcag gcatcgagcc ccagagcaat attccagaga 601 ccccaccccc tggctacctg agtgaagatg gagaaaccag tgaccaccag atgaaccaca 661 gcatggacgc aggttctcca aacctatccc cgaatccgat gtccccagca cataataact 721 tggacctgca gccagttacc tactgcgagc cggccttctg gtgctccatc tcctactacg 781 agctgaacca gcgcgtcggg gagacattcc acgcctcgca gccatccatg actgtggatg 841 gcttcaccga cccctccaat tcggagcgct tctgcctagg gctgctctcc aatgtcaaca 901 ggaatgcagc agtggagctg acacggagac acatcggaag aggcgtgcgg ctctactaca 961 tcggagggga ggtcttcgca gagtgccTCA GTGACAGCGC TATTTTTGTC CAGTCTCCCA 1021 ACTGTAACCA GCGCTATGGC TGGCACCCGG CCACCGTCTG CAAGATCCCA CCAGGATGCA 1081 ACCTGAAGAT CTTCAACAAC CAGGAGTTCG CTGCCCTCCT GGCCCAGTCG GTCAACCAGG 1141 GCTTTGAGGC TGTCTACCAG TTGACCCGAA TGTGCACCAT CCGCATGAGC TTCGTCAAAG 1201 GCTGGGGAGC GGAGTACAGG AGACAGACTG TGACCAGTAC CCCCTGCTGG ATTGAGCTGC 1261 ACCTGAATGG GCCTTTGCAG TGGCTTGACA AGGTCCTCAC CCAGATGGGC TCCCCAAGCA 1321 TCCGCTGTTC CAGTGTGTCT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1381 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1441 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAAA CTTGGTTGGC 1501 ATGCAAAGAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt