Construct: ORF ccsbBroadEn_06549

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010575.1_s300c1, BRDN0000397211
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMAD3 (4088)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4088 SMAD3 SMAD family member 3 NM_005902.4 99.9% 100% 309A>G
2 human 4088 SMAD3 SMAD family member 3 XM_011521559.3 89.5% 89.6% 309A>G;396_397ins132
3 human 4088 SMAD3 SMAD family member 3 NM_001145103.1 87.8% 84.3% (many diffs)
4 human 4088 SMAD3 SMAD family member 3 XM_011521560.1 84.9% 83.4% (many diffs)
5 human 4088 SMAD3 SMAD family member 3 NM_001145102.1 75.2% 75.2% 0_1ins315
6 human 4087 SMAD2 SMAD family member 2 XM_017025746.2 73.6% 89.7% (many diffs)
7 human 4087 SMAD2 SMAD family member 2 NM_001003652.4 69.3% 83.9% (many diffs)
8 human 4087 SMAD2 SMAD family member 2 NM_005901.6 69.3% 83.9% (many diffs)
9 human 4087 SMAD2 SMAD family member 2 XM_005258259.4 69.3% 83.9% (many diffs)
10 human 4087 SMAD2 SMAD family member 2 XM_006722451.4 69.3% 83.9% (many diffs)
11 human 4087 SMAD2 SMAD family member 2 XM_017025745.2 69.3% 83.9% (many diffs)
12 human 4087 SMAD2 SMAD family member 2 XM_024451173.1 69.3% 83.9% (many diffs)
13 human 4088 SMAD3 SMAD family member 3 NM_001145104.1 54.1% 54.1% 0_1ins585
14 human 4087 SMAD2 SMAD family member 2 XM_017025750.2 39.9% 49.2% (many diffs)
15 mouse 17127 Smad3 SMAD family member 3 NM_016769.4 92.9% 100% (many diffs)
16 mouse 17127 Smad3 SMAD family member 3 XM_006510819.2 77.7% 83.7% (many diffs)
17 mouse 17127 Smad3 SMAD family member 3 XM_006510821.3 50.2% 54.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1341
ORF length:
1275
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTC GTCCATCCTG CCTTTCACTC CCCCGATCGT GAAGCGCCTG CTGGGCTGGA
121AGAAGGGCGA GCAGAACGGG CAGGAGGAGA AATGGTGCGA GAAGGCGGTC AAGAGCCTGG
181TCAAGAAACT CAAGAAGACG GGGCAGCTGG ACGAGCTGGA GAAGGCCATC ACCACGCAGA
241ACGTCAACAC CAAGTGCATC ACCATCCCCA GGTCCCTGGA TGGCCGGTTG CAGGTGTCCC
301ATCGGAAGGG GCTCCCTCAT GTCATCTACT GCCGCCTGTG GCGATGGCCA GACCTGCACA
361GCCACCACGA GCTGCGGGCC ATGGAGCTGT GTGAGTTCGC CTTCAATATG AAGAAGGACG
421AGGTCTGCGT GAATCCCTAC CACTACCAGA GAGTAGAGAC ACCAGTTCTA CCTCCTGTGT
481TGGTGCCACG CCACACAGAG ATCCCGGCCG AGTTCCCCCC ACTGGACGAC TACAGCCATT
541CCATCCCCGA AAACACTAAC TTCCCCGCAG GCATCGAGCC CCAGAGCAAT ATTCCAGAGA
601CCCCACCCCC TGGCTACCTG AGTGAAGATG GAGAAACCAG TGACCACCAG ATGAACCACA
661GCATGGACGC AGGTTCTCCA AACCTATCCC CGAATCCGAT GTCCCCAGCA CATAATAACT
721TGGACCTGCA GCCAGTTACC TACTGCGAGC CGGCCTTCTG GTGCTCCATC TCCTACTACG
781AGCTGAACCA GCGCGTCGGG GAGACATTCC ACGCCTCGCA GCCATCCATG ACTGTGGATG
841GCTTCACCGA CCCCTCCAAT TCGGAGCGCT TCTGCCTAGG GCTGCTCTCC AATGTCAACA
901GGAATGCAGC AGTGGAGCTG ACACGGAGAC ACATCGGAAG AGGCGTGCGG CTCTACTACA
961TCGGAGGGGA GGTCTTCGCA GAGTGCCTCA GTGACAGCGC TATTTTTGTC CAGTCTCCCA
1021ACTGTAACCA GCGCTATGGC TGGCACCCGG CCACCGTCTG CAAGATCCCA CCAGGATGCA
1081ACCTGAAGAT CTTCAACAAC CAGGAGTTCG CTGCCCTCCT GGCCCAGTCG GTCAACCAGG
1141GCTTTGAGGC TGTCTACCAG TTGACCCGAA TGTGCACCAT CCGCATGAGC TTCGTCAAAG
1201GCTGGGGAGC GGAGTACAGG AGACAGACTG TGACCAGTAC CCCCTGCTGG ATTGAGCTGC
1261ACCTGAATGG GCCTTTGCAG TGGCTTGACA AGGTCCTCAC CCAGATGGGC TCCCCAAGCA
1321TCCGCTGTTC CAGTGTGTCT TACCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc
1381attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)