Construct: ORF TRCN0000469666
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013476.1_s317c1
- Derived from:
- ccsbBroadEn_13440
- DNA Barcode:
- CTCTACGCATCGAGTATTAGCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC29A4 (222962)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469666
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222962 | SLC29A4 | solute carrier family 29 me... | NM_001300847.2 | 99.9% | 100% | 936T>C |
| 2 | human | 222962 | SLC29A4 | solute carrier family 29 me... | XM_005249658.4 | 99.9% | 100% | 936T>C |
| 3 | human | 222962 | SLC29A4 | solute carrier family 29 me... | NM_001040661.2 | 97.2% | 91.7% | 413_449del;542_546delCAGTG;978T>C |
| 4 | human | 222962 | SLC29A4 | solute carrier family 29 me... | NM_153247.3 | 97.2% | 91.7% | 413_449del;542_546delCAGTG;978T>C |
| 5 | human | 222962 | SLC29A4 | solute carrier family 29 me... | XM_006715667.3 | 97.2% | 91.7% | 413_449del;542_546delCAGTG;978T>C |
| 6 | human | 222962 | SLC29A4 | solute carrier family 29 me... | XM_011515200.2 | 97.2% | 91.7% | 413_449del;542_546delCAGTG;978T>C |
| 7 | human | 222962 | SLC29A4 | solute carrier family 29 me... | XM_011515201.2 | 97.2% | 91.7% | 413_449del;542_546delCAGTG;978T>C |
| 8 | human | 222962 | SLC29A4 | solute carrier family 29 me... | XM_006715668.2 | 65.4% | 65.5% | 0_1ins534;402T>C |
| 9 | mouse | 243328 | Slc29a4 | solute carrier family 29 (n... | NM_146257.2 | 81.2% | 78.8% | (many diffs) |
| 10 | mouse | 243328 | Slc29a4 | solute carrier family 29 (n... | XM_006504706.2 | 81.2% | 78.8% | (many diffs) |
| 11 | mouse | 243328 | Slc29a4 | solute carrier family 29 (n... | XM_006504707.2 | 61.2% | 63% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1614
- ORF length:
- 1548
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctccgtgggg agccagcgcc ttgaggagcc cagcgtggca ggcacaccag 121 acccgggcgt agtgatgagc ttcaccttcg acagtcacca gctggaggag gcggcggagg 181 cggctcaggg ccagggcctt agggccaggg gcgtcccagc tttcacggat actacattgg 241 acgagccagt gcccgatgac cgttatcacg ccatctactt tgcgatgctg ctggctggcg 301 tgggcttcct gctgccatac aacagcttca tcacggacgt ggactacctg catcacaagt 361 acccagggac ctccatcgtg tttgacatga gcctcaccta catcttggtg gcactggcag 421 ctgtcctcct gaacaacgtc ctggtggaga gactgaccct gcacaccagg atcaccgcag 481 catctgcgac gtgtggctgc agctcttctc tcgggaccag gcctacgcca tcaacctggc 541 cgctgtgggc accgtggcct tcggctgcac agcaatccag cttctacggg tacacgggga 601 tgctgcccaa gcggtacacg cagggggtga tgaccgggga gagcacggcg ggcgtgatga 661 tctctctgag ccgcatcctc acgaagctgc tgctgcccga cgagcgcgcc agcacgctca 721 tcttcttcct ggtgtcggtg gcgctggagc tgctgtgttt cctgctgcac ctgttagtgc 781 ggcgcagccg cttcgtgctc ttctatacca cacggccgcg tgacagccac cggggcaggc 841 caggcctggg caggggctat ggctaccgcg tgcaccacga cgttgtcgcc ggggacgtcc 901 acttcgagca cccagccccg gccctggccc ccaacgagtc cccaaaggac agcccagccc 961 acgaggtgac cggcagcggc ggggcctaca tgcgctttga cgtgccgcgg ccaagggtcc 1021 agcgcagctg gcccaccttc agagccctgt tactgcaccg ctacgtggtg gcgcgggtga 1081 tctgggccga catgctctcc atcgccgtga cctacttcat cacgctgtgc ctgttccccg 1141 gcctcgagtc tgagatccgc cactgcatcc tgggcgagtg gctgcccatc ctcatcatgg 1201 ctgtgttcaa cctgtcagac ttcgtgggca agatcctggc agccctgccc gtggactggc 1261 ggggcaccca cctgctggcc tgctcctgcc tgcgtgtggt cttcatcccc ctcttcatcc 1321 tgtgcgtcta ccccagcggc atgcccgccc TCCGTCACCC CGCCTGGCCC TGCATCTTCT 1381 CACTGCTCAT GGGCATCAGC AACGGCTACT TCGGCAGCGT GCCCATGATC CTGGCGGCAG 1441 GCAAAGTGAG CCCCAAGCAG CGGGAGCTGG CAGGGAACAC CATGACCGTG TCCTACATGT 1501 CAGGGCTGAC GCTGGGGTCC GCCGTGGCCT ACTGCACCTA CAGCCTCACC CGCGACGCTC 1561 ACGGCAGCTG CCTGCACGCC TCCACCGCCA ATGGTTCCAT CCTCGCAGGC CTCTGCCCAA 1621 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1681 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1741 TATCTTGTGG AAAGGACGAC TCTACGCATC GAGTATTAGC CCCACGCGTT AAGTCgacaa 1801 tcaacctctg gattacaaaa tttgtgaaag att