Transcript: Human NM_001271685.2

Homo sapiens solute carrier family 35 member A3 (SLC35A3), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
SLC35A3 (23443)
Length:
14282
CDS:
21..1124

Additional Resources:

NCBI RefSeq record:
NM_001271685.2
NBCI Gene record:
SLC35A3 (23443)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001271685.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000434277 GCAACCTCTTTATCGATAATA pLKO_005 963 CDS 100% 15.000 21.000 N SLC35A3 n/a
2 TRCN0000043442 GCGTTATTCCAGAACTTTAAA pLKO.1 218 CDS 100% 15.000 21.000 N SLC35A3 n/a
3 TRCN0000432082 AGAAGGACCTCGTTATCTATC pLKO_005 242 CDS 100% 10.800 15.120 N SLC35A3 n/a
4 TRCN0000043441 GCTACTTATCAGGTCACGTAT pLKO.1 477 CDS 100% 4.950 6.930 N SLC35A3 n/a
5 TRCN0000043440 CCTCAGATTCTCAGCTTGATT pLKO.1 616 CDS 100% 5.625 3.938 N SLC35A3 n/a
6 TRCN0000043438 GCTGTTATTAAGTATGCAGAT pLKO.1 924 CDS 100% 4.050 2.835 N SLC35A3 n/a
7 TRCN0000433417 ACTCTTTCTGTACAGATATAT pLKO_005 1499 3UTR 100% 15.000 9.000 N SLC35A3 n/a
8 TRCN0000043439 CCATCCTTGTAATAACAGCTA pLKO.1 1051 CDS 100% 2.640 1.584 N SLC35A3 n/a
9 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 6771 3UTR 100% 4.950 2.475 Y n/a
10 TRCN0000008902 CCTCCCAAAGTGTTGGGATTA pLKO.1 7230 3UTR 100% 1.080 0.540 Y GPR83 n/a
11 TRCN0000156315 CCTCCCAAAGTGTTGGGATTA pLKO.1 7230 3UTR 100% 1.080 0.540 Y MYORG n/a
12 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2939 3UTR 100% 13.200 6.600 Y LIAS n/a
13 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 8336 3UTR 100% 5.625 2.813 Y KLHL30 n/a
14 TRCN0000166364 CACACACACACACACACACAA pLKO.1 3218 3UTR 100% 4.950 2.475 Y KAAG1 n/a
15 TRCN0000157407 GATCTCGGCTTACTGCAACTT pLKO.1 3330 3UTR 100% 4.950 2.475 Y GTF2IRD2B n/a
16 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 8336 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001271685.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11735 pDONR223 100% 57.8% 57.7% None (many diffs) n/a
2 ccsbBroad304_11735 pLX_304 0% 57.8% 57.7% V5 (many diffs) n/a
3 TRCN0000469720 GAAGGATTTCGGTCTGCAATATCG pLX_317 61.9% 57.8% 57.7% V5 (many diffs) n/a
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