Construct: ORF TRCN0000469727
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003971.1_s317c1
- Derived from:
- ccsbBroadEn_13317
- DNA Barcode:
- CTAACCTTCTGAAAAATGCTGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMNTD1 (160492)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469727
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | NM_001256266.1 | 99.8% | 99.6% | 805A>T |
| 2 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | NM_001145727.3 | 89.4% | 89.2% | 1_102del;907A>T |
| 3 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_011520587.2 | 88.2% | 82.3% | (many diffs) |
| 4 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | NM_001352232.2 | 80% | 79.8% | 1_216del;1021A>T |
| 5 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | NM_001352233.2 | 80% | 79.8% | 1_216del;1021A>T |
| 6 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | NM_001352234.2 | 80% | 79.8% | 1_216del;1021A>T |
| 7 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_011520582.1 | 80% | 79.8% | 1_216del;1021A>T |
| 8 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_011520583.1 | 80% | 79.8% | 1_216del;1021A>T |
| 9 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_011520584.2 | 80% | 79.8% | 1_216del;1021A>T |
| 10 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | NM_152590.3 | 74.9% | 74.7% | 1_291del;1096A>T |
| 11 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_011520588.1 | 74.7% | 74.5% | 0_1ins219;586A>T |
| 12 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | NM_001145728.2 | 71% | 70.9% | 1_354del;1159A>T |
| 13 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_011520575.1 | 71% | 70.9% | 1_354del;1159A>T |
| 14 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_011520576.2 | 71% | 70.9% | 1_354del;1159A>T |
| 15 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_011520577.2 | 71% | 70.9% | 1_354del;1159A>T |
| 16 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_011520578.2 | 71% | 70.9% | 1_354del;1159A>T |
| 17 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_017018891.1 | 71% | 70.9% | 1_354del;1159A>T |
| 18 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_011520579.2 | 68.1% | 67.7% | (many diffs) |
| 19 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | NM_001145729.1 | 61.2% | 61.1% | 1_354del;677_678ins120;1039A>T |
| 20 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_011520580.1 | 61.2% | 61.1% | 1_354del;677_678ins120;1039A>T |
| 21 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XM_017018894.1 | 39.6% | 36.4% | (many diffs) |
| 22 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XR_931277.1 | 38% | (many diffs) | |
| 23 | human | 160492 | LMNTD1 | lamin tail domain containing 1 | XR_931276.1 | 37.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 939
- ORF length:
- 873
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tggggatgga gaagattatt tcctttcttt gtttggtgat tcaaagaaac 121 ttacagcaca ctcaaactac actcagaaaa ctttaaaata cttttctatg attcttgaag 181 aagttggcca atttacctcc agttctcttg gagatgttga aatagctgaa gtgaatgtca 241 agggtttgtt cgtgaagctc attaactctt cccttgacaa agaaatggca attggagatc 301 atattctcca gcaaaatgtg aatggacaaa ccatttcttt gtaccgattc cttccaaaca 361 tcgtaatgca ggcaaattcc acagtaacag tgtgggcagc agcatctgaa gcaaagcatc 421 aacctccatc agattttctt tggaaggaaC AAGACAAGTT TAGAGCAAGT CCTGATTGTA 481 TAACAATCCT GTGCAAACCG AACGGTCAAG CCATTGCGTG GTACACCCCT ATCCACTGGA 541 AGCAAGCGTG GGAAAAATTA GATGCTGACG TTGAATTTAA CAGATGTTCA GTAGTATCTC 601 CAACATTCCG AAAGCGTGTG TTTCAGTGGA CAGCATCTAC AGCTACAATA ACTAAAGAAA 661 AACAAGATCA ACCTAAGAAA GATATCTCAA ATTATCAGGT GGAACAAGCT CAAGTTCTTC 721 TTAAGAGAGA GAAGGAAATC CCACCAACCG TTTTCCCTAA TCGCAGCCCT TGGTGCCAGA 781 ATCCCTATGT CTCTGCACAT CCTTACTGTC CTCTGATTGA ACCACACAAT ACATCCACCG 841 CTGGAGGCAG ATTGGATAGA CAGCCCAGGT CTCGGTCAAC CAGACCTAAT CGAGCCTCAG 901 GGTCTAAGAA AAAGAAGACA TCTGAGTCAC AAAAGCAATA CCCAACTATC TTGTACAAAG 961 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1021 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1081 ACGACTAACC TTCTGAAAAA TGCTGCCTAC GCGTTAAGTC gacaatcaac ctctggatta 1141 caaaatttgt gaaagatt