Construct: ORF TRCN0000469748
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009088.1_s317c1
- Derived from:
- ccsbBroadEn_08758
- DNA Barcode:
- ACCGAGTCGATACGCACCCAGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPAM (57678)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469748
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57678 | GPAM | glycerol-3-phosphate acyltr... | NM_001244949.2 | 99.9% | 99.7% | 11C>A;241C>A |
| 2 | human | 57678 | GPAM | glycerol-3-phosphate acyltr... | NM_020918.6 | 99.9% | 99.7% | 11C>A;241C>A |
| 3 | human | 57678 | GPAM | glycerol-3-phosphate acyltr... | XM_005269998.1 | 99.9% | 99.7% | 11C>A;241C>A |
| 4 | human | 57678 | GPAM | glycerol-3-phosphate acyltr... | XM_024448089.1 | 99.9% | 99.7% | 11C>A;241C>A |
| 5 | mouse | 14732 | Gpam | glycerol-3-phosphate acyltr... | NM_008149.3 | 84.5% | 92.5% | (many diffs) |
| 6 | mouse | 14732 | Gpam | glycerol-3-phosphate acyltr... | XM_006526692.3 | 84.5% | 92.5% | (many diffs) |
| 7 | mouse | 14732 | Gpam | glycerol-3-phosphate acyltr... | XM_006526693.3 | 84.5% | 92.5% | (many diffs) |
| 8 | mouse | 14732 | Gpam | glycerol-3-phosphate acyltr... | XM_006526694.1 | 84.5% | 92.5% | (many diffs) |
| 9 | mouse | 14732 | Gpam | glycerol-3-phosphate acyltr... | XM_011247147.1 | 84.5% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2550
- ORF length:
- 2484
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgaatatgca ctgacccttg gtacaataga tgtttcttat ctgccacatt 121 catcagaata cagtgttggt cgatgtaagc acacaagtga ggaatggggt gagtgtggct 181 ttagacccac catcttcaga tctgcaactt taaaatggaa agaaagccta atgagtcgga 241 aaaggccatt tgttggaaga tgttgttact cctgcactcc ccagagctgg gacaaatttt 301 tcaacaccag tatcccgtct ttgggtttgc ggaatgttat ttatatcaat gaaactcaca 361 caagacaccg cggatggctt gcaagacgcc tttcttacgt tctttttatt caagagcgag 421 atgtgcataa gggcatgttt gccaccaatg tgactgaaaa tgtgctgaac agcagtagag 481 tacaagaggc aattgcagaa gtggctgctg aattaaaccc tgatggttct gcccagcagc 541 aatcaaaagc cgttaacaaa gtgaaaaaga aagctaaaag gattcttcaa gaaatggttg 601 ccactgtctc accggcaatg atcagactga ctgggtgggt gctgctaaaa ctgttcaaca 661 gcttcttttg gaacattcaa attcacaaag gtcaacttga gatggttaaa gctgcaactg 721 agacgaattt gccgcttctg tttctaccag ttcatagatc ccatattgac tatctgctgc 781 tcactttcat tctcttctgc cataacatca aagcaccata cattgcttca ggcaataatc 841 tcaacatccc aatcttcagt accttgatcc ataagcttgg gggcttcttc atacgacgaa 901 ggctcgatga aacaccagat ggacggaaag atgttctcta tagagctttg ctccatgggc 961 atatagttga attacttcga cagcagcaat tcttggagat cttcctggaa ggcacacgtt 1021 ctaggagtgg aaaaacctct tgtgctcggg caggactttt gtcagttgtg gtagatactc 1081 tgtctaccaa tgtcatccca gacatcttga taatacctgt tggaatctcc tatgatcgca 1141 ttatcgaagg tcactacaat ggtgaacaac tgggcaaacc taagaagaat gagagcctgt 1201 ggagtgtagc aagaggtgtt attagaatgt tacgaaaaaa ctatggttgt gtccgagtgg 1261 attttgcaca gccattttcc ttaaaggaat atttagaaag ccaaagtcag aaaccggtgt 1321 ctgctctact ttccctggag caagcgttgt taccagctat acttccttca agacccagtg 1381 atgctgctga tgaaggtaga gacacgtcca ttaatgagtc cagaaatgca acagatgaat 1441 ccctacgaag gaggttgatt gcaaatctgg ctgagcatat tctattcact gctagcaagt 1501 cctgtgccat tatgtccaca cacattgtgg cttgcctgct cctctacaga cacaggcagg 1561 gaattgatct ctccacattg gtcgaagact tctttgtgat gaaagaggaa gtcctggctc 1621 gtgattttga cctggggttc tcaggaaatt cagaagatgt agtaatgcat gccatacagc 1681 tgctgggaaa ttgtgtcaca atcacccaca ctagcaggaa cgatgagttt tttatcaccc 1741 ccagcacaac tgtcccatca gtcttcgaac tcaacttcta cagcaatggg gtacttcatg 1801 tctttatcat ggaggccatc atagcttgca gcctttatgc agttctgaac aagaggggac 1861 tggggggtcc cactagcacc ccacctaacc tgatcagcca ggagcagctg gtgcggaagg 1921 cggccagcct gtgctacctt ctctccaatg aaggcaccat ctcactgcct tgccagacat 1981 tttaccaagt ctgccatgaa acagtaggaa agtttatcca gtatggcatt cttacagtgg 2041 cagagcacga tgaccaggaa gatatcagtc ctagtcttgc tgagcagcag tgggacaaga 2101 agcttccaga acctttgtct tggagaagtg atgaagaaga tgaagacagt gactttgggg 2161 aggaacagcg agattgctac cTGAAGGTGA GCCAATCCAA GGAGCACCAG CAGTTTATCA 2221 CCTTCTTACA GAGACTCCTT GGGCCTTTGC TGGAGGCCTA CAGCTCTGCT GCCATCTTTG 2281 TTCACAACTT CAGTGGTCCT GTTCCAGAAC CTGAGTATCT GCAAAAGTTG CACAAATACC 2341 TAATAACCAG AACAGAAAGA AATGTTGCAG TATATGCTGA GAGTGCCACA TATTGTCTTG 2401 TGAAGAATGC TGTGAAAATG TTTAAGGATA TTGGGGTTTT CAAGGAGACC AAACAAAAGA 2461 GAGTGTCTGT TTTAGAACTG AGCAGCACTT TTCTACCTCA ATGCAACCGA CAAAAACTTC 2521 TAGAATATAT TCTGAGTTTT GTGGTGCTGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 2581 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2641 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACCGA 2701 GTCGATACGC ACCCAGGACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2761 tgaaagatt