Transcript: Mouse XM_006526694.1

PREDICTED: Mus musculus glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Gpam (14732)
Length:
6028
CDS:
250..2733

Additional Resources:

NCBI RefSeq record:
XM_006526694.1
NBCI Gene record:
Gpam (14732)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006526694.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000273786 ATCTCGTATGATCGCATAATC pLKO_005 1309 CDS 100% 13.200 18.480 N Gpam n/a
2 TRCN0000273785 ATTCATGATCACTAGGTTATA pLKO_005 2947 3UTR 100% 13.200 18.480 N Gpam n/a
3 TRCN0000012153 GCCATGTAAAGGCGAGTGATT pLKO.1 3689 3UTR 100% 4.950 6.930 N Gpam n/a
4 TRCN0000012154 GCAGCGAGATTGCTATCTCAA pLKO.1 2346 CDS 100% 0.495 0.693 N Gpam n/a
5 TRCN0000374084 ATGTCGTCATGCATGCTATTC pLKO_005 1841 CDS 100% 10.800 8.640 N Gpam n/a
6 TRCN0000374060 TTGTAGAACTGACTCTTATAT pLKO_005 3062 3UTR 100% 15.000 10.500 N Gpam n/a
7 TRCN0000012156 CCGAATGATGTTGCTGATGAA pLKO.1 1558 CDS 100% 4.950 3.465 N Gpam n/a
8 TRCN0000285061 CCGAATGATGTTGCTGATGAA pLKO_005 1558 CDS 100% 4.950 3.465 N Gpam n/a
9 TRCN0000012157 CGGCAGAAACTCCTAGAGTAT pLKO.1 2689 CDS 100% 4.950 3.465 N Gpam n/a
10 TRCN0000285060 CGGCAGAAACTCCTAGAGTAT pLKO_005 2689 CDS 100% 4.950 3.465 N Gpam n/a
11 TRCN0000012155 GCCACCAGTGTTACTGAGAAT pLKO.1 625 CDS 100% 4.950 3.465 N Gpam n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006526694.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08758 pDONR223 100% 84.5% 92.5% None (many diffs) n/a
2 ccsbBroad304_08758 pLX_304 0% 84.5% 92.5% V5 (many diffs) n/a
3 TRCN0000469748 ACCGAGTCGATACGCACCCAGGAC pLX_317 15.1% 84.5% 92.5% V5 (many diffs) n/a
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