Construct: ORF TRCN0000469794
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014270.1_s317c1
- Derived from:
- ccsbBroadEn_11488
- DNA Barcode:
- CCATCTGATCATCTAAGAGGGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABCA8 (10351)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469794
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10351 | ABCA8 | ATP binding cassette subfam... | XM_011524192.3 | 25% | 25% | 799_3189del |
| 2 | human | 10351 | ABCA8 | ATP binding cassette subfam... | NM_007168.4 | 16.8% | 16.8% | 799_4743del |
| 3 | human | 10351 | ABCA8 | ATP binding cassette subfam... | NM_001288986.1 | 16.4% | 16.4% | 799_4848del |
| 4 | human | 10351 | ABCA8 | ATP binding cassette subfam... | NM_001288985.2 | 16.4% | 16.4% | 799_4863del |
| 5 | human | 10351 | ABCA8 | ATP binding cassette subfam... | XR_002957944.1 | 13.5% | 1_171del;970_5910del | |
| 6 | human | 10351 | ABCA8 | ATP binding cassette subfam... | XR_001752401.2 | 13.3% | 1_171del;970_6000del | |
| 7 | human | 10351 | ABCA8 | ATP binding cassette subfam... | XR_001752402.2 | 13% | (many diffs) | |
| 8 | human | 10351 | ABCA8 | ATP binding cassette subfam... | XM_005256940.4 | 12.6% | 12.6% | 0_1ins183;616_4680del |
| 9 | human | 10351 | ABCA8 | ATP binding cassette subfam... | XR_002957946.1 | 11% | 1_171del;970_7228del | |
| 10 | human | 10351 | ABCA8 | ATP binding cassette subfam... | XR_002957945.1 | 8% | 1_171del;970_9928del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 864
- ORF length:
- 798
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gaagagaaag atcagtgtgt gtcaacaaac ttgggcctta ttatgcaaga 121 actttcttaa aaaatggaga atgaaaagag agtccttaat ggaatggctg aattcattgc 181 tcctactact ttgtttgtat atatatcctc atagtcatca agtaaatgat ttttcttcac 241 tgcttaccat ggacctggga cgggtagata catttaatga atccagattt tctgttgtat 301 acacacctgt caccaacacg acccaacaga taatgaataa agtagcctct actcccttcc 361 tggcaggtaa agaggtcttg ggactgccag atgaggaaag tattaaagaa ttcacagcaa 421 attatcctga agaaatagta agagtcacct ttactaatac atactcatat catttgaagt 481 tcttgctagg acatggaatg ccagcaaaga aggagcacaa ggaCCATACA GCTCATTGTT 541 ATGAAACAAA TGAAGATGTT TACTGTGAAG TTTCAGTATT TTGGAAGGAA GGTTTTGTGG 601 CTCTTCAAGC TGCCATTAAT GCTGCTATTA TAGAAATCAC AACAAATCAC TCAGTGATGG 661 AGGAGCTGAT GTCAGTTACT GGAAAAAATA TGAAGATGCA TTCCTTCATT GGTCAATCAG 721 GAGTTATAAC TGATTTGTAC CTTTTTTCCT GCATTATTTC ATTTTCCTCA TTCATTTACT 781 ATGCATCTGT TAATGTCACA AGAGAGAGGA AAAGGATGAA GGCCTTGATG ACAATGATGG 841 GTCTTCGGGA TTCAGCGTTC TGGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 901 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 961 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CATCTGATCA 1021 TCTAAGAGGG CTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1081 att