Transcript: Human NM_007168.4

Homo sapiens ATP binding cassette subfamily A member 8 (ABCA8), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-09
Taxon:
Homo sapiens (human)
Gene:
ABCA8 (10351)
Length:
5721
CDS:
180..4925

Additional Resources:

NCBI RefSeq record:
NM_007168.4
NBCI Gene record:
ABCA8 (10351)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_007168.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000156204 CCAATCGGATTCCTGGCATAT pLKO.1 3111 CDS 100% 10.800 15.120 N ABCA8 n/a
2 TRCN0000151989 CATGGGTCATAGTATCTGATA pLKO.1 5417 3UTR 100% 4.950 6.930 N ABCA8 n/a
3 TRCN0000419884 GGGCTACTTGGAATGGTTAAA pLKO_005 3033 CDS 100% 13.200 10.560 N Abca8b n/a
4 TRCN0000152073 CAACAAACTTGGGCCTTATTA pLKO.1 207 CDS 100% 15.000 10.500 N ABCA8 n/a
5 TRCN0000151222 GCCCAAGCTTTCTTCAAATTA pLKO.1 4743 CDS 100% 15.000 10.500 N ABCA8 n/a
6 TRCN0000151246 GCACATCCATTAAGGTGATAA pLKO.1 4048 CDS 100% 13.200 9.240 N ABCA8 n/a
7 TRCN0000152102 CCAGTTCTTATGGACATTGTT pLKO.1 3006 CDS 100% 5.625 3.938 N ABCA8 n/a
8 TRCN0000152332 CCAAATTCTGTGTTCCTGTTT pLKO.1 4930 3UTR 100% 4.950 3.465 N ABCA8 n/a
9 TRCN0000155945 CCTGGGAACTTTCTCCTCATT pLKO.1 2734 CDS 100% 4.950 3.465 N ABCA8 n/a
10 TRCN0000154306 CGAAGGCCAAATCACTGCAAT pLKO.1 1700 CDS 100% 4.950 3.465 N ABCA8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007168.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11488 pDONR223 100% 16.8% 16.8% None 799_4743del n/a
2 ccsbBroad304_11488 pLX_304 0% 16.8% 16.8% V5 799_4743del n/a
3 TRCN0000469794 CCATCTGATCATCTAAGAGGGCTC pLX_317 42.9% 16.8% 16.8% V5 799_4743del n/a
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