Construct: ORF TRCN0000469827
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017269.1_s317c1
- Derived from:
- ccsbBroadEn_12791
- DNA Barcode:
- TGTCACCAGTCCGACCCCCCACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SETD3 (84193)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469827
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84193 | SETD3 | SET domain containing 3, ac... | NM_199123.2 | 96.7% | 96.2% | (many diffs) |
| 2 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_011537235.1 | 96.7% | 96.2% | (many diffs) |
| 3 | human | 84193 | SETD3 | SET domain containing 3, ac... | NM_032233.3 | 48.7% | 48.1% | (many diffs) |
| 4 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_011537231.2 | 48.7% | 48.1% | (many diffs) |
| 5 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_011537232.3 | 48.7% | 48.1% | (many diffs) |
| 6 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_017021699.1 | 48.7% | 48.1% | (many diffs) |
| 7 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_017021700.1 | 48.7% | 48.1% | (many diffs) |
| 8 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_011537233.2 | 48.4% | 43.9% | (many diffs) |
| 9 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_017021701.1 | 48.4% | 43.9% | (many diffs) |
| 10 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_005268127.3 | 32% | 29.4% | (many diffs) |
| 11 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_011537234.1 | 32% | 29.4% | (many diffs) |
| 12 | human | 84193 | SETD3 | SET domain containing 3, ac... | XM_017021702.1 | 30.3% | 23.4% | (many diffs) |
| 13 | mouse | 52690 | Setd3 | SET domain containing 3 | XM_006516084.3 | 52.9% | 51.7% | (many diffs) |
| 14 | mouse | 52690 | Setd3 | SET domain containing 3 | XM_017315134.1 | 46.2% | 42.7% | (many diffs) |
| 15 | mouse | 52690 | Setd3 | SET domain containing 3 | XM_017315133.1 | 45.6% | 42.2% | (many diffs) |
| 16 | mouse | 52690 | Setd3 | SET domain containing 3 | NM_028262.3 | 44.8% | 47.1% | (many diffs) |
| 17 | mouse | 52690 | Setd3 | SET domain containing 3 | XM_006516079.3 | 44.3% | 46.5% | (many diffs) |
| 18 | mouse | 52690 | Setd3 | SET domain containing 3 | XM_006516080.3 | 39.5% | 41.2% | (many diffs) |
| 19 | mouse | 52690 | Setd3 | SET domain containing 3 | XM_006516078.3 | 31% | 29.7% | (many diffs) |
| 20 | mouse | 52690 | Setd3 | SET domain containing 3 | XM_006516082.3 | 24.8% | 26% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 942
- ORF length:
- 876
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg taagaagagt cgagtaaaaa ctcagaaatc tggcactggt gctacagcaa 121 ctgtgtcacc aaaggaaatc ttgaacctga ccagtgagct gctgcagaaa tgcagcagtc 181 cggcgcctgg cccaggaaaa gagtgggaag agtatgtgca gatccggact ctggttgaga 241 aaatacggaa aaagcaaaaa ggtctgtccg ttacttttga tggaaaaaga gaagattact 301 ttcctgatct aatgaaatgg gcctctgaaa atggggcttc tgtcgagggt tttgaaatgg 361 ttaacttcaa agaagagggc tttggtttga gagcaacaag agatatcaag gcagaagaat 421 tgtttttatg ggttccacga aaattgctaa tgactgttga atctgctaaa aattcagtgt 481 tggggccctt atattctcaa gaccgaatcc ttcaagccat gggaaacatc gcactggCCT 541 TTCATTTGCT GTGTGAGCGA GCCAGCCCTA ACTCCTTCTG GCAGCCCTAT ATTCAAACCC 601 TCCCCAGTGA ATATGACACT CCTCTCTACT TTGAAGAAGA TGAAGTTCGG TATCTTCAGT 661 CCACACAAGC TATACATGAT GTCTTCAGCC AGTATAAAAA CACAGCTCGA CAGTACGCCT 721 ACTTCTATAA AGTCATCCAG ACCCATCCTC ATGCCAACAA ACTACCCTTG AAGGATTCTT 781 TCACTTACGA GGACTACAGG TGGGCAGTCT CTTCTGTTAT GACGAGGCAA AACCAAATTC 841 CCACAGAGGA TGGTTCCCGC GTGACCCTGG CTCTGATTCC TTTATGGGAT ATGTGTAACC 901 ACACCAACGG CCTGGTAACG ACCTCTTCCC CTGTGTTGTG TTGCCCAACT TTCTTGTACA 961 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1021 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1081 AGGACGATGT CACCAGTCCG ACCCCCCACA CACGCGTTAA GTCgacaatc aacctctgga 1141 ttacaaaatt tgtgaaagat t