Transcript: Human XM_017021699.1

PREDICTED: Homo sapiens SET domain containing 3, actin histidine methyltransferase (SETD3), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SETD3 (84193)
Length:
2730
CDS:
19..1803

Additional Resources:

NCBI RefSeq record:
XM_017021699.1
NBCI Gene record:
SETD3 (84193)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017021699.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000122097 CTTATATTCTCAAGACCGAAT pLKO.1 441 CDS 100% 4.050 5.670 N SETD3 n/a
2 TRCN0000143148 CTTCTGTTATGACGAGGCAAA pLKO.1 764 CDS 100% 4.050 5.670 N SETD3 n/a
3 TRCN0000276530 GATGTCTTCAGCCAGTATAAA pLKO_005 631 CDS 100% 15.000 10.500 N SETD3 n/a
4 TRCN0000276580 AGATTACTTTCCTGATCTAAT pLKO_005 246 CDS 100% 13.200 9.240 N SETD3 n/a
5 TRCN0000175757 GAGGTCAAACTTTGGACATTT pLKO.1 1285 CDS 100% 13.200 9.240 N Setd3 n/a
6 TRCN0000143640 GCTTTGGTTTGAGAGCAACAA pLKO.1 332 CDS 100% 4.950 3.465 N SETD3 n/a
7 TRCN0000276531 GCTTTGGTTTGAGAGCAACAA pLKO_005 332 CDS 100% 4.950 3.465 N SETD3 n/a
8 TRCN0000121628 GAAGAAGATGAAGTTCGGTAT pLKO.1 586 CDS 100% 4.050 2.835 N SETD3 n/a
9 TRCN0000285593 GAAGAAGATGAAGTTCGGTAT pLKO_005 586 CDS 100% 4.050 2.835 N SETD3 n/a
10 TRCN0000140464 GCAACTGTGTCACCAAAGGAA pLKO.1 70 CDS 100% 3.000 2.100 N SETD3 n/a
11 TRCN0000143535 GAAATCTTGAACCTGACCAGT pLKO.1 88 CDS 100% 2.640 1.584 N SETD3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017021699.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12791 pDONR223 100% 48.7% 48.1% None (many diffs) n/a
2 ccsbBroad304_12791 pLX_304 0% 48.7% 48.1% V5 (many diffs) n/a
3 TRCN0000469827 TGTCACCAGTCCGACCCCCCACAC pLX_317 46.3% 48.7% 48.1% V5 (many diffs) n/a
4 ccsbBroadEn_12790 pDONR223 100% 35.3% 34.5% None (many diffs) n/a
5 ccsbBroad304_12790 pLX_304 0% 35.3% 34.5% V5 (many diffs) n/a
6 TRCN0000474620 GCGGACCCCCACCCACACATATAC pLX_317 38.6% 35.3% 34.5% V5 (many diffs) n/a
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