Construct: ORF TRCN0000469988
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017155.3_s317c1
- Derived from:
- ccsbBroadEn_14529
- DNA Barcode:
- CAAGCGACGGGCTTTCGGACTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACVR2A (92)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469988
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92 | ACVR2A | activin A receptor type 2A | NM_001278579.2 | 99.9% | 100% | 354G>A |
| 2 | human | 92 | ACVR2A | activin A receptor type 2A | NM_001616.5 | 99.9% | 100% | 354G>A |
| 3 | human | 92 | ACVR2A | activin A receptor type 2A | NM_001278580.1 | 78.8% | 78.9% | 0_1ins324;30G>A |
| 4 | mouse | 11480 | Acvr2a | activin receptor IIA | NM_007396.4 | 94.5% | 99.6% | (many diffs) |
| 5 | mouse | 11480 | Acvr2a | activin receptor IIA | XM_006497623.3 | 93% | 98% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1605
- ORF length:
- 1539
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg agctgctgca aagttggcgt ttgccgtctt tcttatctcc tgttcttcag 121 gtgctatact tggtagatca gaaactcagg agtgtctttt ctttaatgct aattgggaaa 181 aagacagaac caatcaaact ggtgttgaac cgtgttatgg tgacaaagat aaacggcggc 241 attgttttgc tacctggaag aatatttctg gttccattga aatagtgaaa caaggttgtt 301 ggctggatga tatcaactgc tatgacagga ctgattgtgt agaaaaaaaa gacagccctg 361 aagtatattt ttgttgctgt gagggcaata tgtgtaatga aaagttttct tattttccag 421 agatggaagt cacacagccc acttcaaatc cagttacacc taagccaccc tattacaaca 481 tcctgctcta ttccttggtg ccacttatgt taattgcggg gattgtcatt tgtgcatttt 541 gggtgtacag gcatcacaag atggcctacc ctcctgtact tgttccaact caagacccag 601 gaccaccccc accttctcca ttactaggtt tgaaaccact gcagttatta gaagtgaaag 661 caaggggaag atttggttgt gtctggaaag cccagttgct taacgaatat gtggctgtca 721 aaatatttcc aatacaggac aaacagtcat ggcaaaatga atacgaagtc tacagtttgc 781 ctggaatgaa gcatgagaac atattacagt tcattggtgc agaaaaacga ggcaccagtg 841 ttgatgtgga tctttggctg atcacagcat ttcatgaaaa gggttcacta tcagactttc 901 ttaaggctaa tgtggtctct tggaatgaac tgtgtcatat tgcagaaacc atggctagag 961 gattggcata tttacatgag gatatacctg gcctaaaaga tggccacaaa cctgccatat 1021 ctcacaggga catcaaaagt aaaaatgtgc tgttgaaaaa caacctgaca gcttgcattg 1081 ctgactttgg gttggcctta aaatttgagg ctggcaagtc tgcaggcgat acccatggac 1141 aggttggtac ccggaggtac atggctccag aggtattaga gggtgctata aacttccaaa 1201 gggatgcatt tttGAGGATA GATATGTATG CCATGGGATT AGTCCTATGG GAACTGGCTT 1261 CTCGCTGTAC TGCTGCAGAT GGACCTGTAG ATGAATACAT GTTGCCATTT GAGGAGGAAA 1321 TTGGCCAGCA TCCATCTCTT GAAGACATGC AGGAAGTTGT TGTGCATAAA AAAAAGAGGC 1381 CTGTTTTAAG AGATTATTGG CAGAAACATG CTGGAATGGC AATGCTCTGT GAAACCATTG 1441 AAGAATGTTG GGATCACGAC GCAGAAGCCA GGTTATCAGC TGGATGTGTA GGTGAAAGAA 1501 TTACCCAGAT GCAGAGACTA ACAAATATTA TTACCACAGA GGACATTGTA ACAGTGGTCA 1561 CAATGGTGAC AAATGTTGAC TTTCCTCCCA AAGAATCTAG TCTATGCCCA ACTTTCTTGT 1621 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1681 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1741 GAAAGGACGA CAAGCGACGG GCTTTCGGAC TAAAACGCGT TAAGTCgaca atcaacctct 1801 ggattacaaa atttgtgaaa gatt